Contents 1
Advanced organotypic in vitro model systems for host-microbial coculture / Raehyun Kim 1
Abstract 1
1. Introduction 1
2. Brief Introduction to the Human Microbiota and Human Epithelium In Vivo 2
2.1. Human Microbiota 2
2.2. Human Epithelial Cells and Their Communications with Immune Cells 3
3. Considerations for Developing an In Vitro Coculture Model Systems 3
3.1. Coculture Formats 5
3.2. Host Cell Types and Sources 6
3.3. Bacteria 7
3.4. Conducting Coculture Experiments and Analyses 7
4. Current Developments of Organotypic Coculture Model Systems 8
4.1. Intestine 8
4.2. Skin 15
4.3. Female Reproductive Tract (FRT) 16
4.4. Respiratory Tract 17
4.5. Urinary Tract 18
5. Challenges and Opportunities in Organotypic Bacterial Cocultures 19
6. Summary 22
Author Contributions 22
References 22