Contents 1
Development of Gut-Mucus chip for intestinal absorption study / Seung Yeon Lee ; Yujeong Lee ; Nakwon Choi ; Hong Nam Kim ; Bumsang Kim ; Jong Hwan Sung 1
Abstract 1
1. Introduction 1
2. Materials and Methods 3
2.1. Gut-Mucus Chip Fabrication 3
2.2. Cell Culture 3
2.3. Mucin Coating 3
2.4. Staining and Quantification of Coated Mucin 3
2.5. Permeability Assay 4
2.6. Synthesis of Polymer Particle 4
2.7. Evaluation of Mucosal Adhesion of Particles 5
2.8. pH Sensitivity Comparison 5
3. Results and Discussion 5
3.1. Fabrication of Gut-Mucus Chip 5
3.2. The Role of the Coated Mucin Layer 6
3.3. pH Sensitivity of Polymer Particles 8
3.4. Adhesion of Polymer Particles for Drug Delivery 9
3.5. Effect of Flow Rate on Mucosal Adhesion and Drug Absorption Permeability 9
4. Conclusion 11
References 12