Contents 1
Paper-based radial flow assay integrated to portable isothermal amplification chip platform for colorimetric detection of target DNA / Tai-Yong Kim ; Sanha Kim ; Jae Hwan Jung ; Min-Ah Woo 1
Abstract 1
1. Introduction 1
2. Results and Discussion 2
2.1. Design and Fabrication of Microchip-Based Detection Platform 2
2.2. Description of Paper-Based Colorimetric Detection Strategy 2
2.3. Optimizing Condition of the Temperature Controller 3
2.4. Padlock Probe Verification and Optimization of One-Step Enzymatic Reaction Parameters 5
2.5. Selectivity Test of the Proposed Assay 6
2.6. Sensitivity and Recovery Test of the Proposed Assay 6
3. Conclusions 8
4. Materials and Methods 8
4.1. Chemicals and Reagents 8
4.2. PDMS Microchip Fabrication and Optimizing Condition of the Temperature Controller 8
4.3. Optimization of One-Step Enzyme Reaction Parameters 9
4.4. Selectivity Test of the Proposed Method 9
4.5. Quantitative Analysis for DNA Samples and Reproducibility Evaluation of the Developed Method 10
Author Contributions 10
References 10