Contents 1
Development of an extracellular matrix plate for drug screening using patient-derived tumor organoids / Yong Hun Jung ; Kyungwon Park ; Minseop Kim ; Hyunjik Oh ; Dong-Hee Choi ; Jinchul Ahn ; Sat-byol Lee ; Kyuhwan Na ; Byung Soh Min ; Jin-A. Kim ; Seok Chung 1
Abstract 1
1. Introduction 1
2. Results 2
2.1. Development of EM Plate 2
2.2. Distribution of Tumor Organoids 2
2.3. Confirmation of Consistency in the Fluorescence Expression in EM Plate 4
2.4. Drug Screening of Tumor Organoids in the EM Plate 4
3. Discussion 4
4. Methods 7
4.1. Human Tissues 7
4.2. Organoid Culture 7
4.3. Extracellular Matrix Plate Preparation and Organoid Culture 7
4.4. Drug Screening 7
4.5. Viability Analysis(Live/Dead Staining and Cell-Titer-Glow Assay) 8
4.6. Expression intensity calculation 8
4.7. Organoid-Distribution Evaluation 8
4.8. Statistical Analysis 8
References 8