Contents 1
Immunogenicity monitoring cell chip incorporating finger-actuated microfluidic and colorimetric paper-based analytical functions / Kyung Won Lee ; Eun Kyeong Yang ; Yujeong Oh ; Eunhye Park ; Kwan Young Jeong ; Hyun C. Yoon 1
Abstract 1
1. Introduction 1
1.1. Design and Fabrication of the Immunogenicity-Testing Chip 2
1.2. Development of PAD for Analysis Module 3
1.3. Validation of the Finger-Actuation Function of the Assay Chip 3
1.4. Optimization of Sensing Time for the Developed System 5
1.5. Cell Activation and Viability Tests in the Cell Culture Chamber 5
1.6. Calibration Study of Hydrogen Peroxide Detection in Cell Growth Media 6
1.7. Cell-secreted Hydrogen Peroxide Determination Using the Developed Chip 7
2. Conclusions 7
3. Experimental Procedures 9
3.1. Materials and Apparatus 9
3.2. Fabrication of the Immunogenicity-Testing Chip 9
3.3. Confirmation of the Cell Viability in the Chip 9
3.4. Construction of the PAD for Hydrogen Peroxide Analysis 9
3.5. Determination of the Sensing Time and Volume of the Transferred Medium 9
3.6. Calibration of PAD 10
3.7. In situ analysis of the Cell-Secreted Hydrogen Peroxide 10
References 10