Contents 1
Fabrication of hydrogel microchannels using aqueous two-phase printing for 3D blood brain barrier / Hyunjik Oh ; Minjin Kang ; Eunji Bae ; Yonghun Jung ; Jinhui Cho ; Joscillyn Poirier ; Jong Sung Kim ; John P. Frampton ; Nakwon Choi ; Seok Chung 1
Abstract 1
1. Introduction 1
2. Results and Discussion 2
2.1. 3D BBB on a Chip Technology Overview 2
2.2. Experimental Design 2
2.3. Fabrication and System Configuration 3
2.4. Effects of Flow Rate Variation 4
2.5. Effects of Ink Phase Type Variation 5
2.6. Selection Process for a 3D BBB Matrix 5
2.7. Effect of Calcium Chloride Treatment 8
2.8. Immunofluorescence Analysis 10
2.9. BBB Permeability Test 11
3. Conclusion 12
4. Materials and Methods 12
4.1. Cell Culture 12
4.2. Preparation of Cell Pellet-Embedded Alginate-Collagen-Matrigel Matrix Phases 13
4.3. Ink Phase Material for 3D Printing 13
4.4. Sulfo-SANPAH Coating 13
4.5. ATPP Systems 13
4.6. Cell viability and Surface Morphology Analysis 14
4.7. Vascular Permeability 14
4.8. Immunofluorescence Staining 14
References 14