Microfl uidics is fl ourishing due to its signifi cant applications in life sciences and biomedical engineering. One of the key challenges in microfl uidics is the manipulation and control of fl uids within microscale channels. Capillary force-driven fl ows provide a potential solution to this challenge by eliminating the need for an external power source. Capillary force- driven fl ows are particularly useful for the reproducible and reliable quantitative analysis of single-molecule DNA. We have designed several microfl uidic devices that employ capillary force to enhance the deposition of DNA molecules onto a positively charged glass surface from a sample solution. The optimization of specifi c dimensions within the microfl uidic device resulted in increased effi ciency of DNA deposition. Shortening the microchannel length reduced fl ow resistance and decreasing the microchannel height enhanced capillary force. Additionally, increasing the outlet reservoir capacity achieves mechanical equilibrium in situations where fl uid fl ow is maximized. These optimizations served to maximize capillary force and improve DNA deposition on the glass surface. The developed device represents an ultra-sensitive platform for quantitative DNA analysis and rapid, accurate point-of-care testing with a minimum detection limit achieved. In conclusion, our work demonstrates the potential of capillary force-driven microfl uidics for the reproducible and effi cient manipulation of fl uids within microscale channels.