Contents 1
Microfluidic device to maximize capillary force driven flows for quantitative single-molecule DNA analysis / Taesoo Kim ; Kyubong Jo 1
Abstract 1
1. Introduction 1
2. Results and Discussion 2
2.1. Microfluidic Device Design 2
2.2. DNA Stretching by Various Channel Heights 3
2.3. DNA Deposition by Microchannel Length and the Outlet Reservoir Volume 6
2.4. Flow-Through Volume by Outlet Reservoir Volume 6
3. Conclusion 7
4. Materials and Methods 7
4.1. Chemicals and Materials 7
4.2. Fluorescence Microscopy 8
4.3. Fabrication of PDMS Microfluidic Device 8
4.4. Preparation of Positively Charged Glass Surface 8
4.5. Detection of dsDNA Molecules in Microfluidic Devices 8
4.6. Flow-Through Volume Measurement 8
References 8