In this study, an FPGA-based sealer inspection system was developed to inspect the sealer applied to install vehicle glass on a carbody. The sealer is a liquid or paste-like material that promotes adhesion such as sealing and waterproofing for mounting and assemblingvehicle parts to a car body. The system installed in the existing vehicle design parts line does not detect the sealer in the glass rotationsection and takes a long time to process. This study developed a line laser camera sensor and an FPGA vision signal processing moduleto solve this problem. The line laser camera sensor was developed such that the resolution and speed of the camera for data acquisitioncould be modified according to the irradiation angle of the laser. Furthermore, it was developed considering the mountability of the entiresystem to prevent interference with the sealer ejection machine. In addition, a vision signal processing module was developed using theZynq-7020 FPGA chip to improve the processing speed of the algorithm that converted the profile to the sealer shape image acquiredfrom a 2D camera and calculated the width and height of the sealer using the converted profile. The performance of the developed sealerapplication inspection system was verified by establishing an experimental environment identical to that of an actual automobile productionline. The experimental results confirmed the performance of the sealer application inspection at a level that satisfied the requirementsof automotive field standards.