Understanding the impact of material choice and lighting in exhibition spaces is crucial for enhancing visitor experience and emotional engagement. This study explores the influence of material, lighting color temperature, and lighting type on emotional responses in exhibition environments. Utilizing four materials (marble, metal, wood, plaster) and combinations of two lighting color temperatures (3500K, 5000K) and two lighting types (diffuse, spotlight), 16 virtual exhibition spaces were created for evaluation. The results indicate that wood material evokes relaxing and soft emotions, whereas metal is associated with an interesting and unique mood. Low color temperature lighting (3500K) was perceived as more unique and pleasant compared to neutral color temperature lighting (5000K). Diffuse lighting generally created a soft and relaxing environment. More importantly, interaction effects were observed between material, lighting color temperature, and lighting type, indicating that they need to be strategically chosen to elicit specific emotional experiences. This study underscores the importance of considering various factors in exhibition space design to enrich visitor experience and suggests further research with diverse materials, lighting conditions, and participant demographics to deepen understanding of these elements' interplay in exhibition settings.