표제지
제출문
N,N-Dimethylguanidine Sulfate
목차
요약문 9
Summary 11
I. 서론 13
II. 연구내용 및 결과 14
1. 연구내용 14
급성 경구 독성시험 15
2. 연구결과 16
가. 시험물질 16
(1) 시험물질 및 부형제 16
(2) 시험물질 조제 16
(3) 시험물질 분석 16
나. 재료 및 방법 17
(1) 시험동물 17
(2) 사육환경 18
(3) 군분리 및 개체식별 18
(4) 시험물질의 투여 20
(5) 관찰 및 검사 항목 20
(6) 통계처리 20
다. 한계시험 결과 21
(1) 치사동물 및 반수치사약량 (LD50)(이미지참조) (Table 1) 21
(2) 일반증상관찰결과 (Table 2) 21
(3) 체중측정결과 (Table 3) 21
(4) 부검결과소견 (Table 4) 21
(5) 병리소견 21
라. 추가시험 결과 (예비시험 및 본시험) 22
(1) 치사동물 및 반수치사약량(LD50)(이미지참조) (Table 5) 22
(2) 일반증상관찰결과 (Table 6) 22
(3) 체중측정결과 (Table 7) 22
(4) 부검결과소견 (Table 8) 22
마. 고찰 23
피부자극시험 61
가. 연구방법 62
(1) 시험물질 및 대조물질 62
(2) 시험물질 조제 62
(3) 시험물질 분석 62
(4) 시험계 63
(5) 사육환경 및 관리 63
(6) 시험방법 64
(7) 관찰 및 검사 항목 65
(8) 피부 반응의 평가 및 자극성의 판정 66
나./다. 결과 66
(1) 일반증상 및 사망률 (Table 2) 66
(2) 체중변화 (Table 3) 66
(3) 적용부위 관찰 (Table 4) 67
다./라. 고찰 67
소핵시험 71
가. 연구방법 72
(1) 시험물질 및 대조물질 72
나. 재료 및 방법 73
(1) 시험계 73
(2) 군분리 및 개체식별 73
(3) 사육환경 74
(4) 시험방법 75
다. 시험결과 76
(1) 동물의 사육관리 76
(2) 시험물질의 투여 77
(3) 소핵 관찰 결과 77
라. 고찰 77
III. 결론 81
급성경구독성시험 81
피부자극시험 81
소핵시험 82
참고 문헌 83
판권지 84
Table 1. Mortality of limit test (Group summary) 24
Table 2. Clinical signs of limit test (Group summary) 24
Table 3. Changes of the mean body weights of limit test (Group summary) 25
Table 4. Necropsy findings of limit test (Group summary) 25
Table 5. Mortality of preliminary test. (Group summary) 26
Table 6. Clinical signs of preliminary test. (Group summary) 26
Table 7. Changes of the mean body weights of preliminary test.(Group summary) 27
Table 8. Necropsy findings of preliminary test. (Group summary) 28
Table 9. Mortality of main test. (Group summary) 28
Table 10. Clinical signs of main test. (Group summary) 29
Table 11. Changes of the mean body weights of main test (Group summary) 30
Table 12. Necropsy findings of main test.(Group summary) 31
Table 1. 피부반응평가표 66
Table 2. Mortality and clinical signs 68
Table 3. Body weights 68
Table 4. Results of skin reaction 69
Table 1. Micronucleus test of N,N-Dimethylguanidine Sulfate in ICR mice (group summary) 78
Table 2. Body weights of animals (group summary) 78
Appendix 1.1. Clinical signs of male in limit test (Individual) 32
Appendix 1.2. Clinical signs of female in limit test (Individual) 33
Appendix 2.1. Body weights of male in limit test (Individual) 34
Appendix 2.2. Body weights of female in limit test (Individual) 35
Appendix 3.1. Necropsy findings of male in limit test Individual) 36
Appendix 3.2. Necropsy findings of female in limit test (Individual) 37
Appendix 4.1. Clinical signs of male in preliminarty test (Individual) 38
Appendix 4.2. Clinical signs of female in preliminarty test (Individual) 40
Appendix 5.1. Body weights of male in preliminarty test (Individual) 42
Appendix 5.2. Body weights of female in preliminarty test (Individual) 43
Appendix 6.1. Necropsy findings of male in preliminarty test (Individual) 44
Appendix 6.2. Necropsy findings of female in preliminarty test (Individual) 46
Appendix 7.1. Clinical signs of male in main test (Individual) 48
Appendix 7.2. Clinical signs of female in main test (Individual) 50
Appendix 8.1. Body weights of male in main test (Individual) 52
Appendix 8.2. Body weights of female in main test (Individual) 53
Appendix 9.1. Necropsy findings of male in main test (Individual) 54
Appendix 9.2. Necropsy findings of female in main test (Individual) 56
Appendix 1. Micronucleus test of N,N-Dimethylguanidine sulfate in ICR mice (Individual data) 79
Appendix 2. Body weights of animals (individual data) 80
Fig 1. No specific lesion of stomach (100X, Animal ID :1201) 58
Fig 2. Pyknosis and eosinophilic cytoplasm of hepatocyte (200X, Animal ID :1201) 58
Fig 3. Macrophage phagocytosis of dark brown pigment in sinusoid (200X, Animal ID :1203) 59
Fig 4. Macrophage phagocytosis of dark brown pigment in sinusoid and vein (200X, Animal ID :1202) 59
Figure 1. 피부처치부위 65
Figure 2. Skin after topical application at 24 hr 70
Figure 3. Skin after topical application at 72 hr 70