[Title Page] 1
연구진 2
Contents 3
Abstract 5
I. Background 7
II. Materials and Methods 12
1. Potential Habitat Analysis 12
A. Geographic scope 12
B. Environmental Variables 12
C. Models 16
2. Genetic Analysis 18
A. Sample collection and DNA extraction 18
B. Species and Sex Identification 20
C. Genotypic profile construction 21
3. International Collaborations 22
A. Multilateral Collaboration Support 22
B. Amur leopard conservation network 23
4. Long-term conservation plan for Amur leopards 23
III. Results 24
1. Potential habitat Analysis 24
A. Amur leopards 24
B. Amur Gorals 25
2. Genetic analysis 27
A. Sample collection and species & sex identification 27
B. Microsatellite genotyping 29
3. International Collaborations 31
A. Multilateral Collaboration Support 31
B. Amur leopard conservation network 32
4. Long-term conservation plan 35
A. Vision 35
B. Goal 35
C. Conservation strategies 35
IV. Discussion and Conclusion 38
References 40
Appendix 41
Appendix I. NEASPEC Activity plan for biodiversity and nature conservation in 2023-2024 41
Appendix II. Presentations for the international workshop for Amur leopard ex-situ conservation 43
[Back Cover] 64
Table 2-1. Summary of environmental variables changing history 15
Table 2-2. original ESA landcover code, discription, and 4 class we used 17
Table 3-1. Microsatellite panel for individual identification and genetic... 29
Table 3-2. Genotyping success and error rates of 12 selected... 30
Table 3-3. Amur tiger genetic diversity estimates using 10 microsatellite loci.... 30
Figure 2-1. Genetic experiments in Russia 19
Figure 3-1. Result of the Amur leopard potential habitat analysis 24
Figure 3-2. Historical range of Amur leopard 25
Figure 3-3. Result of the Amur goral potential habitat analysis(binary map) 26
Figure 3-4. Result of the Amur goral potential habitat analysis in LLNP 27
Figure 3-5. Molecular species identification 28
Figure 3-6. Molecular sexing 28
Figure 3-7. NEASPEC 26th Senior Officials Meeting 31
Figure 3-8. International Workshop for Amur leopard Ex-situ Conservation 33
Figure 3-9. Suggestions to improve the current enclosures in the Research... 35
Figure 3-10. Strategies and procedures for the leopard recovery in South Korea 37