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[표제지]=0,1,1

제출문=1,2,1

요약문=2,3,10

Summary=12,13,11

Contents=23,24,3

목차=26,27,3

List of Figures=29,30,6

List of Tables=35,36,1

제1장 서론=36,37,4

제2장 국내외 기술개발 현황=40,41,3

제3장 연구개발수행 내용 및 결과=42-1,44,1

제1절 분류 및 분포 조사=43,45,1

1. 재료 및 방법=43,45,1

가. 형태적 분류=43,45,1

나. RAPD 변이 분석=43,45,3

다. 서식지 분포=45,47,2

라. 서식지 환경=46,48,2

2. 결과 및 고찰=48,50,1

가. 형태적 분류=48,50,2

나. RAPD 변이 분석=50,52,4

다. 서식지 분포=54,56,14

라. 서식지 환경=68,70,8

제2절 생식주기 조사=76,78,1

1. 재료 및 방법=76,78,2

2. 결과 및 고찰=78,80,5

제3절 산란유발=83,85,1

1. 재료 및 방법=83,85,1

2. 결과 및 고찰=83,85,10

제4절 난발생 및 치패사육=93,95,1

1. 재료 및 방법=93,95,1

2. 결과 및 고찰=93,95,12

제5절 나팔고둥 유생의 먹이섭취 및 소화지수=105,107,1

1. 재료 및 방법=105,107,1

2. 결과 및 고찰=105,107,9

제6절 먹이공급량과 수용밀도에 따른 치패의 성장=114,116,1

1. 재료 및 방법=114,116,5

2. 결과 및 고찰=119,121,3

제7절 나팔고둥 정자 및 유생의 동결보존=122,124,1

1. 재료 및 방법=122,124,1

가. 정자=122,124,1

나. 부화유생=122,124,2

2. 결과 및 고찰=123,125,1

가. 정자=123,125,7

나. 부유유생=130,132,6

제8절 나팔고둥의 환경내성=136,138,1

1. 재료 및 방법=136,138,1

2. 결과 및 고찰=137,139,5

제9절 나팔고둥의 포식특성=142,144,1

1. 재료 및 방법=142,144,3

2. 결과 및 고찰=145,147,3

제10절 모패 사육=148,150,1

1. 재료 및 방법=148,150,1

2. 결과 및 고찰=148,150,9

제11절 종합고찰=157,159,9

제4장 연구개발목표 달성도 및 대외기여도=166,168,5

제5장 연구개발결과의 활용계획=170-1,173,3

제6장 참고문헌=172-1,176,11

List Of Tables

Table 1. Sequence Of Arbitrary Primers Used In The Experiment=44,46,1

Table 2. Genetic Distance Index Between Populations Of Based On Band Sharing Analysis For Opd-10 Mer Primers=53,55,1

Table 3. Relationships Between Water Temperature And Time(Day) Required To Reach To Each Developmental Stage From Fertilization=96,98,1

Table 4. Range Of Temperatures, Algal Diets, Feeding Rates And Larval Densities Tested For The Larval Charonia Lampas Sauliae=115,117,1

Table 5. Linear Regression Equations For Shell Length Of Conch Veliger From Each Feeding Rates=118,120,1

Table 6. Constituents And Osmolality Of Cryoprotectants And Diluents Used In The Larva Cryopreservation Of Charonia Lampas Sauliae=125,127,1

Table 7. Surval Rate Of Charonia Lampas Sauliae Under Various Water Temperature Conditions=138,140,1

Table 8. Surval Rate Of Charonia Lampas Sauliae Under Various Salinity Conditions=139,141,1

Table 9. Surval Rate Of Charonia Lampas Sauliae Larvae Under Various Water Temperature Conditions=140,142,1

Table 10. Surval Rate Of Charonia Lampas Sauliae Larvae Under Various Salinity Conditions=141,143,1

Table 11. Measurement Of The Trumpet Shells Used In The Experiment=143,145,1

Table 12. Measurement Of The Various Preys Used In The Experiment=144,146,1

List Of Figures

Fig. 1. Map Showing The Sampling Stations=47,49,1

Fig. 2. Photograph Of Charonia Lampas Sauliae And Charonia Lampas Macilenta Scale Bar : 10cm=49,51,1

Fig. 3. Electrophoretic Patterns Of 1% Agarose Gel Stained By Ethidium Bromide Showing Total DNAs From Gastropods=51,53,1

Fig. 4. Random Amplified Polymorphic DNAs From Gastropods Using Arvitrary OPA-9 And OPA-10=52,54,1

Fig. 5. Geographical Distribution Of Charonia Lampas Sauliae In Jeju-Do, Korea=55,57,1

Fig. 6. Relationships Between Shell Length And Total Weight Of Charonia Lampas Sauliae=57,59,1

Fig. 7. Relationships Between Shell Length And Total Weight Of Charonia Lampas Macilenta=58,60,1

Fig. 8. Relationships Between Shell Length And Shell Width Of Charonia Lampas Sauliae=59,61,1

Fig. 9. Relationships Between Shell Length And Shell Width Of Charonia Lampas Macilenta=60,62,1

Fig. 10. Relationships Between Shell Width And Total Weight Of Charonia Lampas Sauliae=61,63,1

Fig. 11. Relationships Between Shell Width And Total Weight Of Charonia Lampas Macilenta=62,64,1

Fig. 12. Distribution Of Shell Length(㎜) Charonia Lampas Sauliae And Charonia Lampas Macilenta=64,66,1

Fig. 13. Distribution Of Total Weight(g) Charonia Lampas Sauliae And Charonia Lampas Macilenta=65,67,1

Fig. 14. Frequency Of Charonia Sp. Males To Females=66,68,1

Fig. 15. Frequency Of Charonia Sp. Males To Females According To Shell Length=67,69,1

Fig. 16. Variations Of Water Temperature On Different Sea Areas During The Research Period=69,71,1

Fig. 17. Variations Of Salinity On Different Sea Areas During The Research Period=70,72,1

Fig. 18. Variations Of pH On Different Sea Areas During The Research Period=71,73,1

Fig. 19. Variations Of Dissolved Oxygen On Different Sea Areas During The Research Period=72,74,1

Fig. 20. Variations Of Total Nitrogen On Different Sea Areas During The Research Period=73,75,1

Fig. 21. Variations Of Total Phosphorus On Different Sea Areas During The Research Period=74,76,1

Fig. 22. Variations Of Suspended Solids On Different Sea Areas During The Research Period=75,77,1

Fig. 23. Anatomy Of The Charonia Sauliae Adults=77,79,1

Fig. 24. The Ovary Of The Trumpet Shell At Different Sample Time=81,83,1

Fig. 25. The Testis Of The Trumpet Shell=82,84,1

Fig. 26. Copulation Behavior Of Female And Male Individuals=85,87,1

Fig. 27. The Trumpet Shell, Charonia Lampas Sauliae With Its Egg Mass=86,88,1

Fig. 28. The Egg Sac Of Charonia Lampas Sauliae=87,89,1

Fig. 29. Number Of Egg Capsules Of Charonia Lampas Sauliae During Experimental Period=88,90,1

Fig. 30. Relationships Between Capsule Length And Capsule Breadth Of Charonia Lampas Sauliae Egg Capsules=89,91,1

Fig. 31. Relationships Between Capsule Weight And Number Of Egg On Charonia Lampas Sauliae Egg Capsules=90,92,1

Fig. 32. Relationship Between Body Weight(g) And Number Of Egg Capsules=91,93,1

Fig. 33. Relationship Between Body Weight(g) And Egg Mass(g)=92,94,1

Fig. 34. Egg Developmental Stage Of Trumpet Shell=95,97,1

Fig. 35. Influence Of Water Temperature On Egg Development In Trumpet Shell, Charonia Lampas Sauliae=97,99,1

Fig. 36. Survival Rate Of Egg Mass On Developmental Stage=98,100,1

Fig. 37. Relationships Between Shell Length And Shell Width Of Charonia Lampas Sauliae Larvae=99,101,1

Fig. 38. Growth Of Charonia Lampas Sauliae Larvae On Different Water Temperature Tank=101,103,1

Fig. 39. Survival Rate Of Charonia Lampas Sauliae Larvae On Different Water Temperature Tank=102,104,1

Fig. 40. Settled Spat Of Charonia Lampas Sauliae=103,105,1

Fig. 41. Relationships Between Shell Length And Shell Width Of Charonia Lampas Sauliae Larvae=104,106,1

Fig. 42. Larval Charonia Lampas Sauliae Used In The Experiment=107,109,1

Fig. 43. Ingestion Index Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Three Hours After Fed=108,110,1

Fig. 44. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Three Hours After Fed=109,111,1

Fig. 45. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Five Hours After Fed=110,112,1

Fig. 46. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Eight Hours After Fed=111,113,1

Fig. 47. Digestion Index Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Three Hours After Fed=112,114,1

Fig. 48. Digestion Index Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Three Hours After Fed=113,115,1

Fig. 49. Growth Of Larval Charonia Lampas Sauliae At Different Feeding Rates=116,118,1

Fig. 50. Survival Rate Of Larval Charonia Lampas Sauliae At Different Feeding Rates=117,119,1

Fig. 51. Growth Of Larval Charonia Lampas Sauliae At Various Larval Densities=120,122,1

Fig. 52. Survival Rate Of Larval Charonia Lampas Sauliae At Various Larvae Densities=121,123,1

Fig. 53. Sperm And Larva Of Trumpet Shell Used In The Experiment=124,126,1

Fig. 54. Freezing Rate Graph Used In The Experiment=126,128,1

Fig. 55. Variation Of Sperm Activity Index And Survival Rate In Trumpet Shell, Charonia Lampas Sauliae Sperm Stored At 4℃ With Six Diluents=128,130,1

Fig. 56. Variation Of Survival Rate In Trumpet Shell, Charonia Lampas Sauliae Sperm Stored At -196℃ With Dimethyl Sulfoxide Concentration=129,131,1

Fig. 57. The Survival Rate(Mean±SE) Of Trumpet Shell, Charonia Lampas Sauliae Larvae, Which Had Been Frozen For 1 Hr., And Then Passed Through 1Hr After Being Thawed=131,133,1

Fig. 58. The Survival Rate Of Trumpet Shell, Charonia Sauliae Larvae, Which Had Been Frozen For 1 Hr. Using DMSO As Cyroprotectant, And Then Were Determined At Different Elapsed Time After Being Thawed=132,134,1

Fig. 59. The Survival Rate Using DMSO At The Concentration With The Storage For 1 Day, 7 Days And 15 Days=133,135,1

Fig. 60. Survival Rate Of Post- Thawed Larvae In Each Developmental Stage Of Charonia Lampas Sauliae. Fe: Fertilization Egg=134,136,1

Fig. 61. Microscope Photograph Of Post-Thawed Larvae Of Trumpet Shell, Charonia Lampas Sauliae=135,137,1

Fig. 62. Predation Of Charonia Sp. During Experimental Period=146,148,1

Fig. 63. Predation Of Charonia Lampas Sauliae=147,149,1

Fig. 64. Change In The Total Weight(g) Of Charonia Lampas Sauliae And Charonia Lampas Macilenta=150,152,1

Fig. 65. Relationships Between Shell Length And Shell Width Of Charonia Lampas Sauliae=151,153,1

Fig. 66. Relationships Between Shell Length And Total Weight Of Charonia Lampas Sauliae=152,154,1

Fig. 67. Relationships Between Shell Width And Shell Total Weight Ofcharonia Lampas Sauliae=153,155,1

Fig. 68. Relationships Between Shell Length And Shell Width Of Charonia Lampas Macilenta=154,156,1

Fig. 69. Relationships Between Shell Length And Total Weight Of Charonia Lampas Macilenta=155,157,1

Fig. 70. Relationships Between Shell Width And Total Weight Of Charonia Lampas Macilenta=156,158,1

영문목차

[title page etc.]=0,1,12

Summary=12,13,11

Contents=23,24,6

List Of Figures=29,30,6

List Of Tables=35,36,1

Chapter 1. Introduction=36,37,4

Chapter 2. The Present State Of Technique=40,41,3

Chapter 3. The Contents And Result Of The Study=42-1,44,1

Section 1. Research Of Classification And Distribution=43,45,1

1. Material And Methods=43,45,1

1) Classification=43,45,3

2) Distribution=45,47,2

3) Habitat Environment=46,48,2

2. Result And Discussion=48,50,1

1) Classification=48,50,6

2) Distribution=54,56,14

3) Habitat Environment=68,70,8

Section 2. Reproductive Cycle=76,78,1

1. Material And Methods=76,78,2

2. Result And Discussion=78,80,5

Section 3. Spawning Induction=83,85,1

1. Material And Methods=83,85,1

2. Result And Discussion=83,85,10

Section 4. Egg Development And Spat Rearing=93,95,1

1. Material And Methods=93,95,1

2. Result And Discussion=93,95,12

Section 5. Larval Feeding And Digestion Index=105,107,1

1. Material And Methods=105,107,1

2. Result And Discussion=105,107,9

Section 6. Growth According To Food Organism Concentration And Larval Densities=114,116,1

1. Material And Methods=114,116,5

2. Result And Discussion=119,121,3

Section 7. Cryopreservation=122,124,1

1. Material And Methods=122,124,1

1) Cryopreservation Of Sperm=122,124,1

2) Cryopreservation Of Larvae=122,124,2

2. Result And Discussion=123,125,1

1) Cryopreservation Of Sperm=123,125,7

2) Cryopreservation Of Larvae=130,132,6

Section 8. Environmental Tolerance=136,138,1

1. Material And Methods=136,138,1

2. Result And Discussion=137,139,5

Section 9. Predation=142,144,1

1. Material And Methods=142,144,3

2. Result And Discussion=145,147,3

Section 10. Rearing Of Brood Stock=148,150,1

1. Material And Methods=148,150,1

2. Result And Discussion=148,150,9

Section 11. Discussion=157,159,9

Chapter 4. Research Achievement And Contribution=166,168,5

Chapter 5. Application=170-1,173,3

Chapter 6. Reference=172-1,176,11

칼라목차

jpg

Fig. 29. Number Of Egg Capsules Of Charonia Lampas Sauliae During Experimental Period=88,90,1

Fig. 44. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Three Hours After Fed=109,111,1

Fig. 45. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Five Hours After Fed=110,112,1

Fig. 46. Digestion Rate(%) Of Six Microalgal Species By Charonia Lampas Sauliae Larvae In Eight Hours After Fed=111,113,1

Fig. 62. Predation Of Charonia Sp. During Experimental Period=146,148,1

42-1,44,1

170-1,173,1

172-1,176,1

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