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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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결과 내 검색 동의어 포함

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I. 서론 4

II. 실험재료 및 방법 5

1. 실험재료 5

2. 실험방법 5

1) 세포 배양 및 방사선 조사 5

2) RNA의 분리 6

2) RNA시료에서 DNA제거 6

3) 역전사 및 중합효소연쇄반응 (RT-PCR) 6

4) Denaturing polyacrylamide gel 전기영동 6

5) cDNA의 재증폭 7

6) Agarose gel로부터 DNA의 회수 7

7) 중합효소연쇄반응 산물의 cloning 7

8) DNA 염기서열 결정 및 분석 7

III. 결과 7

1. 차등발현 mRNA의 확인 7

2. 차등발현 유전자의 염기서열 결정 9

3. 차등발현유전자의 확인 9

IV. 고찰 10

V. 결론 12

참고문헌 12

ABSTRACT 16

초록보기

 Because of the increase in use of radiations for detection and treatment of various diseases in dental and medical clinics, and also the ozon layer is becoming thinner due to air pollutions, there is higher chance that human beings are getting exposured to radiations. Therefore, understandings on the interactions of radiationa and living organisms are need to be enhanced. In this study we used modem molecular biological techniques to identify radiation-resistance induction related genes. We induced radiation resistance in human melanoma cells and isolated total RNA from control and experimental groups. RNA's were reverse transcribed and polymerase chian reaction was performed. Sequencing grade electrophoresis analysis revealed differentially expressed mRNA's. We have chosen two bands showed increased expression in experimental group and named RR1 and RR2, respectively. These bands were isolated from polyacrylaminde gel and reamplified using same primers used for reverse transcriptiona and polymerase chain reactions. These amplified DNA fragments were cloned into pGEM-T vector and sequenced. RR1 contained 457 bp DNA and RR2 contained 159 bp DNA. Similarity was searched using basic local alignment search tool (BLAST) against Genbank, EMBL and DDBJ banks. RR1 showed 99% identity with human H3.3 histone mRNA, and RR2 was identical to cDNA for human mitochondrial ubiquinone-binding protein in compared regions. It seems that RR1 is involved in maintaining DNA structure, thus DNA repair would be more feasible after DNA damage by irradiation. RR2 seems to be involved in free radical scavenging, thus contribute resistance to radiation.

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