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국회도서관 홈으로 정보검색 소장정보 검색

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국문요약

abstract

목차

I. 서론 13

II. 방법 16

[가토를 이용한 in vivo study] 16

1. 실험군 16

a. PRK 군 16

b. LASEK 군 16

2. 현미경적 관찰을 통한 재생정도 비교 17

3. 광학 및 전자현미경적 관찰 17

4. 면역세포화학법 18

a. PCNA (proliferating cell nuclear antigen) 18

b. Fibronectin 18

c. EGFR (epidermal growth factor receptor) 19

[각막상피세포 배양을 통한 in vitro study ] 20

1. 대조군과 실험군 20

a. 대조군 20

b. 실험군 21

2. 배양세포수의 비교 22

III. 결과 23

[가토를 이용한 in vivo study] 23

1. 현미경적 관찰을 통한 재생정도 비교 23

2. 광학 및 전자현미경적 관찰 23

3. 면역세포화학법 24

[각막상피세포 배양을 통한 in vitro study ] 25

1. 각막 상피세포수의 비교 25

IV. 고찰 26

V. 결론 29

참고문헌 31

그림목차

Figure 1. Photographs of fluorescein stained epithelial defect in rabbit cornea at 24 hours after operation. 37

Figure 2. Photographs of fluorescein stained epithelial defect in rabbit cornea at 48 hours after operation. 38

Figure 3. Photographs of fluorescein stained epithelial defect in rabbit cornea at 72 hours after operation. 39

Figure 4. The changes of corneal epithelial wound healing area (%) relative to time in each group. 40

Figure 5. The comparison of wound healing rate in LASEK and PRK groups. 41

Figure 6. Hematoxylin-Eosin stain in rabbit corneal epithelia (x 400) at 1 day after operation. 42

Figure 7. Hematoxylin-Eosin stain of rabbit corneal epithelia (x 400) at 3 day after operation. 43

Figure 8. Hematoxylin-Eosin stain in rabbit corneal epithelia (x 400) at 7 day after operation. 44

Figure 9. Hematoxylin-Eosin stain in rabbit corneal epithelia (x 400) at 10 day after operation. 45

Figure 10. Transmission electron micrographs of rabbit corneal epithelia at 3 days after operation. (x7000) 46

Figure 11. Transmission electron micrographs of rabbit corneal epithelia at 7 days after operation (x10000). 47

Figure 12. Immunocytochemical staining for PCNA in rabbit cornea at 3 days after operation (x200) 48

Figure 13. Immunocytochemical staining for fibronectin in rabbit cornea at 3 days after operation (x200) 49

Figure 14. Immunocytochemical staining for EGFR in rabbit cornea at 3 days after operation (x200) 50

Figure 15. The evaluation for the effect of alcohol treated corneal epithelial cell in porcine corneal epithelial cell culture. 51