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국문목차

표제지=0,1,4

ABSTRACT=i,5,2

목차=iii,7,2

LIST OF SCHEME=v,9,1

LIST OF TABLES=vi,10,1

LIST OF FIGURES=vii,11,2

I. 서론=1,13,1

1. 연구배경=1,13,6

2. 연구목적=6,18,2

II. 재료 및 방법=8,20,1

1. 문주란의 추출물 및 용매 분획물 제조=8,20,1

2. 세포 배양=8,20,1

3. 세포독성 실험=9,21,1

4. Analysis for DNA fragmentation=9,21,1

5. Cell cycle analysis=9,21,2

6. RNA extraction=10,22,1

7. RT-PCR analysis=10,22,1

8. MX accumulation=10,22,4

III. 결과=14,26,1

1. 문주란의 세포 증식 억제 효과=14,26,1

2. 문주란의 HL-60 세포 apoptosis 유도 효과=14,26,2

3. 문주란의 HL-60/MX2 세포 다약물내성 극복 효과=15,27,1

3.1 문주란의 HL-60/MX2 세포 apoptosis 유도 효과=15,27,1

3.2 문주란의 HL-60/MX2 세포 다약물내성 조절제 효과=15,27,15

IV. 고찰=30,42,4

V. 참고문헌=34,46,8

도식목차

Scheme1. Procedure for solvent extraction of Crinum asiaticum=12,24,1

표목차

Table1. The sequences of primers and the expected sizes of the RT-PCR products=13,25,1

Table2. PCR conditions of Bcl-2, Bax, MDR1, MRP, BCRP and β₂-MG=13,25,1

Table3. Inhibitory effects of the 80 ％ MeOH extract or several solvent fractions from C. asiaticum on the growth of HL-60 cells and HL-60/MX2 cells=17,29,1

Table4. Inhibitory effects of the 80 ％ MeOH extract or several solvent fractions from C. asiaticum on the growth of HEL-299 cells=18,30,1

그림목차

Figure1. DNA fragmentation by the 80 ％ MeOH extract or several solvent fractions from C. asiaticum in HL-60 cells=19,31,1

Figure2. The degree of apoptosis represented as the DNA content measured by flow cytometric analysis in HL-60 cells=20,32,1

Figure3. RT-PCR analysis of Bcl-2 and Bax expression in the HL-60 cells treated with several solvent fractions from C. asiaticum=21,33,1

Figure4. DNA fragmentation by the 80％ MeOH extract or several solvent fractions from C. asiaticum in HL-60/MX2 cells=22,34,1

Figure5. The degree of apoptosis represented as the DNA content measured by flow cytometric analysis in HL-60/MX2 cells=23,35,1

Figure6. RT-PCR analysis of Bcl-2 and Bax expression in the HL-60/MX2 cells treated with 80 ％ MeOH extract or several solvent fractions from C. asiaticum=24,36,1

Figure7. RT-PCR analysis of MDR-1, MRP and BCRP expression in the HL-60/MX2 cells treated with 80％ MeOH extract or several solvent fractions from C. asiaticum=25,37,1

Figure8. Flow cytometry analysis of mitoxantrone accumulation by the 80％ MeOH extract or several solvent fractions of C. asiaticum in HL-60/MX2 cells=26,38,1

Figure9. Flow cytometry analysis of mitoxantrone accumulation by cyclospolin A and the 80 ％ MeOH extract or several solvent fractions of C. asiaticum in HL-60/MX2 cells=27,39,1

Figure10. Flow cytometry analysis of mitoxantrone accumulation by sulfinpyrazon and the 80％ MeOH extract or several solvent fractions of C. asiaticum in HL-60/MX2 cells=28,40,1

Figure11. Flow cytometry analysis of mitoxantrone accumulation by verapamil and the 80％ MeOH extract or several solvent fractions of C. asiaticum in HL-60/MX2 cells=29,41,1

칼라목차

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Figure1. DNA fragmentation by the 80 ％ MeOH extract or several solvent fractions from C. asiaticum in HL-60 cells=19,31,1

Figure3. RT-PCR analysis of Bcl-2 and Bax expression in the HL-60 cells treated with several solvent fractions from C. asiaticum=21,33,1

Figure4. DNA fragmentation by the 80％ MeOH extract or several solvent fractions from C. asiaticum in HL-60/MX2 cells=22,34,1

Figure6. RT-PCR analysis of Bcl-2 and Bax expression in the HL-60/MX2 cells treated with 80 ％ MeOH extract or several solvent fractions from C. asiaticum=24,36,1

Figure7. RT-PCR analysis of MDR-1, MRP and BCRP expression in the HL-60/MX2 cells treated with 80％ MeOH extract or several solvent fractions from C. asiaticum=25,37,1

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