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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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ABSTRACT 6

Ⅰ. 서론 8

Ⅱ. 재료 및 방법 10

1. 재료 10

1) 시약 10

2) 약물의 추출 및 제조 10

3) 실험동물 11

2. 방법 11

1) 비장세포의 분리 11

2) 복강 대식세포의 분리 및 배양 12

3) MTT 분석 12

4) LDH Leakage 분석 13

5) 비장세포에서 사이토카인 측정 13

6) 아질산 농도의 측정 13

7) PGE₂와 COX 활성 측정 14

8) iNOS와 COX-2의 Western blot 14

9) 비장세포 증식능 측정 15

10) 자연살해세포의 살해능 측정 15

11) 통계 분석 16

Ⅲ. 실험 결과 17

1. 사군자탕 추출물이 세포 생존율에 미치는 영향 17

2. 사군자탕 추출물이 LDH 유리에 미치는 영향 17

3. 사군자탕 추출물이 NO 생성에 미치는 영향 20

4. 사군자탕 추출물이 PGE₂ 생성에 미치는 영향 20

5. 사군자탕 추출물이 COX-2 활성에 미치는 영향 23

6. 사군자탕 추출물이 iNOS와 COX-2 발현에 미치는 영향 23

7. 사군자탕 추출물이 Th1 사이토카인 생성에 미치는 영향 26

8. 사군자탕 추출물이 Th2 사이토카인 생성에 미치는 영향 26

9. 사군자탕 추출물이 비장세포 증식능에 미치는 영향 32

10. 사군자탕 추출물이 자연살해세포의 활성에 미치는 효과 32

Ⅳ. 고찰 35

Ⅴ. 결론 40

참고문헌 41

국문초록 45

List of Table

Table 1. Oriental crude drug composition of Sagunza-tang (400 g) 11

List of Figure

Fig. 1. Cell viability and LDH leakage in Sagunja-tang extract-treated peritoneal macrophages. Peritoneal macrophages (5×105(이미지참조)/well plate) from Balb/c mice were incubated for 24 h in the presence or absence of the extract at indicated concentrations. Cell viability and... 18

Fig. 2. Cell viability in Sagunja-tang extract-treated peritoneal macrophages. Peritoneal macrophages (5×105(이미지참조)/well plate) from Balb/c mice were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without LPS (100... 19

Fig. 3. Effects of Sagunja-tang extract on NO production by LPS-stimulated peritoneal macrophages. Peritoneal macrophages (5×105(이미지참조)/well plate) from Balb/c mice were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated... 21

Fig. 4. Effects of Sagunja-tang extract on PGE₂ production by LPS-stimulated peritoneal macrophages. Cells (5×105(이미지참조)/㎖) from Balb/c mice were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without 100 ng/... 22

Fig. 5. Effects of Sagunja-tang extract on COX activity in LPS-stimulated peritoneal macrophages. Cells (5×105(이미지참조)/) ㎖ were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without 100 ng/㎖ LPS for 18 h. COX activity... 24

Fig. 6. Effects of Sagunja-tang extract on expression of iNOS and COX-2 in LPS-stimulated peritoneal macrophages. Cells (5×105(이미지참조)/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without LPS (100 ng/㎖) for 18... 25

Fig. 7. Effects of Sagunja-tang extract on productions of IFN-γ (Th1 cytokine) in Balb/c mouse spleen cells stimulated with PHA. Cells (5×105(이미지참조)/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without PHA (2... 27

Fig. 8. Effects of Sagunja-tang extract on productions of IL-2 (Th1 cytokine) in Balb/c mouse spleen cells stimulated with PHA. Cells (5×105(이미지참조)/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without PHA (2... 28

Fig. 9. Effects of Sagunja-tang extract on productions of IL-4 (Th2 cytokine) in Balb/c mouse spleen cells stimulated with PHA. Cells (5×105/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without PHA (2... 29

Fig. 10. Effects of Sagunja-tang extract on productions of IL-5 (Th2 cytokine) in Balb/c mouse spleen cells stimulated with PHA. Cells (5×105(이미지참조)/㎖) were pretreated with or without each extract at indicated concentrations for 2 h, and then incubated with or without PHA (2... 30

Fig. 11. Effects of Sagunja-tang extract on productions of IL-13 (Th2 cytokine) in Balb/c mouse spleen cells stimulated with PHA. Cells (5×105(이미지참조)/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without PHA (2... 31

Fig. 12. Effects of Sagunja-tang extract on the spleen cell proliferation of Balb/c mouse by PHA treatment. Cells (5×105(이미지참조)/㎖) were pretreated with or without the extract at indicated concentrations for 2 h, and then incubated with or without 2 ㎍/㎖ PHA for 48 h. Cell... 33

Fig. 13. Effects of in vivo administration with Sagunja-tang extract administration on the cytotoxic activity of natural killer cells. Mice were administrated with or without Sagunja-tang extract (25-400 mg/kg·body weight) for 1 week , and then killed the mice with... 34

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