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ABSTRACT

목차

I. 서론 18

II. 연구사 21

1. 혐기성 소화 분해 과정 및 관여 미생물 21

(1) 가수분해단계 23

(2) 산생성단계 26

(3) 메탄생성단계 29

2. 반추위 내 섬유소분해균 31

(1) Fibrobacter succinogenes 32

(2) Ruminococcus 속 32

3. BMP (Biochemical Methance Potenial) test 35

III. 재료 및 방법 37

실험 1. Cellulose를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 37

1. 공시시료 37

2. 혼합 methanogens와 반추위 섬유소 분해균 공시 37

3. 혼합 methanogens와 반추위 섬유소 분해균 배양 37

4. 실험설계 40

5. BMP(Biochemical methane potential) test 41

6. 조사항목 및 분석방법 43

실험 2. Fibrobacter succinogenes 처리에 따른 첨가가 메탄발생량에 미치는 영향 46

1. 공시시료 46

2. 공시균주 46

3. 공시균주의 배양배지 및 배양방법 46

4. 실험설계 47

5. BMP(Biochemical methane potential) test 47

6. 조사항목 및 분석방법 48

실험 3. 돈분슬러지를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 50

1. 공시시료 50

2. 혼합 methanogens와 반추위 섬유소 분해균 공시 50

3. 혼합 methanogens와 반추위 섬유소 분해균 배양 50

4. 실험설계 50

5. BMP(Biochemical methane potential) test 50

6. 조사항목 및 분석방법 51

IV. 결과 및 고찰 52

실험 1. Cellulose를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 52

1. pH 52

2. 총고형물(TS, total solid) 53

3. 휘발성고형물(VS, volatile solid) 54

4. TCODcr 57

5. 가스발생량 및 메탄발생량 58

실험 2. Fibrobacter succinogenes 처리에 따른 첨가가 메탄발생량에 미치는 영향 64

1. 화학적 성상변화 64

2. 가스발생량 및 메탄발생량 66

실험 3. 돈분슬러지를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 68

1. pH 68

2. 총고형물(TS, total solid) 69

3. 휘발성고형물(VS, volatile solid) 70

4. TCODcr 73

5. 가스발생량 및 메탄발생량 74

V. 결과 및 요약 80

실험 1. Cellulose를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 80

실험 2. Fibrobacter succinogenes 처리에 따른 첨가가 메탄발생량에 미치는 영향 81

실험 3. 돈분슬러지를 기질로 이용한 혐기소화에서 혼합 methanogens와 반추위 섬유소 분해균 첨가가 메탄발생량에 미치는 영향 82

VI. 인용문헌 83

List of Tables

Table 1. Volatile Fatty acids commonly found during the anaerobic digestion of sludge 27

Table 2. Classification of methanogens based on the species and substrate 30

Table 3. Composition of a the medium for aceto-clastic methanogens in Experiment 1 38

Table 4. Dehority's artificial medium 39

Table 5. The experimental design of Experiment 1 40

Table 6. Characteristics of anaerobic media for biochemical methane potential test 42

Table 7. The esperimental design of Experiment 2 47

Table 8. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on final pH in the BMP test using cellulose as a substrate in Experiment 1 52

Table 9. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on total solid (g/L)in the BMP test using cellulose as a substrate in Experiment 1 53

Table 10. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on volatile solid(g/L) variations in the BMP test using cellulose as a substrate in Experiment 1 54

Table 11. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on volatile solid digestion efficiency (%) in the BMP test using cellulose as a substrate in Experiment 1 55

Table 12. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on TCODcr(g/L)in the BMP test using cellulose as a substrate in Experiment 1 57

Table 13. Effects of supplementary levels of methanogens and rumen cellulolytic bacteria on cumulative total gas and methane production in the BMP test using cellulose as a substrate in Experiment 1 58

Table 14. Effects of supplementation of Fibrobacter succinogens on the changes of Ph, CODcr, TS, VS and VS digestion in the BMP test using cellulose as a substrate in Experiment 2 64

Table 15. Effects of supplementation of Fibrobacter succinogens on total gas and methane yields in the BMP test using cellulose as a substrate in Experiment 2 66

Table 16. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on final pH in the BMP test using pig slurry as a substrate in Experiment 3 68

Table 17. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on total solid(g/L)in the BMP test using pig slurry as a substrate in Experiment 3 69

Table 18. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on volatile solid(g/L)in the BMP test using pig slurry as a substrate in Experiment 3 70

Table 19. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on volatile solid digestion(%) efficiency in the BMP test using pig slurry as a substrate in Experiment 3 71

Table 20. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on TCODcr(g/L)in the BMP test using pig slurry as a substrate in Experiment 3 73

Table 21. Effects of supplementary levels of mixed methanogens and rumen cellulolytic bacteria on total gas nad methane production in the BMP test using pig slurry as a substrate in Experiment 3 74

List of Figures

Fig 1. Metabolic pathway involved in anaerobic digestion 22

Fig 2. The floe of carbon and hydrogen in anaerobic digestion 28

Fig 3. A measuring system of gas production 44

Fig 4. Cumulative production of total gas from BMP test added with 1% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 59

Fig 5. Cumulative production of methane from BMP test added with 1% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 60

Fig 6. Cumulative production of total gas from BMP test added with 3% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 61

Fig 7. Cumulative production of methane from BMP test added with 3% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 61

Fig 8. Cumulative production of total gas from BMP test added with 5% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 62

Fig 9. Cumulative production of methane from BMP test added with 5% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 1 62

Fig 10. Cumulative production of total gas from BMP test added with 5% of Fibrobacter succinogenes in Experiment 2 67

Fig 11. Cumulative production of methane from BMP test added with 5% of Fibrobacter succinogenes in Experiment 2 67

Fig 12. Cumulative production of total gas from BMP test added with 1% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 75

Fig 13. Cumulative production of methane from BMP test added with 1% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 76

Fig 14. Cumulative production of total from BMP test added with 3% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 77

Fig 15. Cumulative production of methane from BMP test added with 3% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 77

Fig 16. Cumulative production of total gas from BMP test added with 5% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 78

Fig 17. Cumulative production of methnae from BMP test added with 5% of the cultures of methanogens and rumen cellulolytic bacteria in Experiment 3 79

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