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Title Page

Contents

ABSTRACTS 4

Introduction 6

Materials and Methods 8

Cell culture and chemicals 8

Light irradiation and establishment of indirect irradiation model 8

Cell Viability 9

Western blot analysis 9

Enzyme-Linked Immunosorbent assay for PGE₂ 10

Detection of ROS Formation 10

Cytokine profiling 10

Statistical analysis 11

Results 14

Determination of LPS concentration 14

The direct/indirect effects of 635nm irradiation on P. gingivalis LPS-induced COX-2 expression and PGE₂ release. 16

The direct/indirect effects of 635nm irradiation on the ROS level 19

The direct/indirect effects of 635nm irradiation on cytokine production 21

Discussion 27

Reference 29

국문초록 31