본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

목차보기

Title Page

Abstract

Contents

CHAPTER 1. Optimized Extraction and Purification Conditions for Bioactive Compounds from Natural Plants 16

1.1. Introduction 17

1.1.1. Extraction Methodologies 17

1.1.2. Purification using Solid-Phase Extraction 21

1.1.3. Application of New Solid-Phase Extraction Sorbent 22

1.1.4. Investigation of New Method 30

1.2. Theoretical Background 33

1.2.1. Assisted Extraction Methods 33

1.2.2. Response Surface Methodology 34

1.2.3. Solid-Phase Extraction 35

1.2.4. Molecular Imprinted Technology 35

1.3. Experimental 36

1.3.1. Optimization of Extraction Conditions 36

1.3.2. Response Surface Methodology 37

1.3.3. Solid-Phase Extraction 39

1.3.4. Molecularly Imprinted Solid-Phase Extraction 39

1.3.5. Multi-Phase Extraction 42

1.4. Results and Discussions 44

1.4.1. Optimization of Extraction Conditions 44

1.4.2. Optimized Extraction Condition by RSM 52

1.4.3. Purification of flavones by SPE 57

1.4.4. Molecularly Imprinted Solid-Phase Extraction 59

1.4.5. Multi-Phase Extraction 64

1.5. References 68

CHAPTER 2. Porous Ionic Liquid-Immobilized Polymer Sorbents 77

2.1. Introduction 78

2.1.1. Different Ionic Liquid-Modified Sorbents 78

2.1.2. Evaluation of the Adsorption Efficiency of Ionic Liquid-Immobilized Polymer 81

2.1.3. Ionic Liquid-Immbilized Polymers in SPE Method 84

2.1.4. Ionic Liquid-Immbilized Microporous Polymers with MIP 87

2.1.5. Application of Ionic Liquid-Immbilized Porous Polymers in MPE 88

2.2. Experimental 90

2.2.1. Adsorption Isotherms 90

2.2.2. Solid-Phase Extraction 91

2.2.3. Molecularly Imprinted Ionic Liquid-Modified Porous Polymers 95

2.2.4. Ionic Liquid-Modified Microporous Polymers 98

2.2.5. Application of Ionic Liquid-Immbilized Porous Polymers in MPE 99

2.3. Results and Discussion 101

2.3.1. Adsorption Isotherms 101

2.3.2. SPE with IL-polymer 113

2.3.3. Molecular Imprinted Ionic Liquid-Immobilized Polymers 123

2.3.4. Ionic Liquid-Modified Microporous Polymers 126

2.3.5. Application of Ionic Liquid-Immbilized Porous Polymers in MPE 128

2.4. References 133

Conclusions 138

Summary in Korean 139

Summary in Chinese 140

Appendix 141

Appendix 1. Removal of Toxic Essential Oils in Chamaecyparis obtusa by Headspace Single-Drop Microextraction 141

Appendix 2. Adsorption Isotherm of Caffeine and Theophylline on the Metal- Organic Frameworks 142

Appendix 3. Investigation of Ofloxacin Enantioseparation by Ligand Exchange Chromatography 143

Appendix 4. Ionic liquid-Assisted Extraction and Separation of Astaxanthin from Shrimp Waste 144

List of Publications 145

List of Tables

Table 1-1. Summary of MIP-SPE applications in natural plant 24

Table 1-2. Independent variables their levels used for BBD 38

Table 1-3. Different kinds of imprinted polymers and the imprint effect 42

Table 1-4. Extracted amounts of LQ with different solvents 44

Table 1-5. Extraction amount of four compounds by using liquid-solid extraction 48

Table 1-6. Extraction amount of four compounds by using ultrasonic-assistant and microwave-assistant extraction 50

Table 1-7. BBD experimental design with the independent variables 52

Table 1-8. Analysis of variance of the experimental results of the BBD 54

Table 1-9. Elution strength of several solvents 57

Table 1-10. Extracted amounts of quercitrin, myricetin and amentoflavone with different solvents in washing step (MISPE cartridge) 63

Table 1-11. Adsorbed amount of standard solvent with different sorbents 64

Table 2-1. Different characteristic between polymer and silica as the substrate of sorbent 80

Table 2-2. Effect of time on the adsorption of lactic acid 102

Table 2-3. Effect of the initial lactic acid concentration on the adsorption of lactic acid 104

Table 2-4. Effect of pH on the adsorption of lactic acid 106

Table 2-5. Adsorption of target compounds on differences sorbents 108

Table 2-6. Amounts of caffeine and theophylline in commercial tea drinks 115

Table 2-7. Extracted amounts of liquiritin and glycyrrhizin by different SPE sorbent in the steps of washing and elution 116

Table 2-8. Extracts amounts of the two compounds from three commercial medicines 117

Table 2-9. Extracted amounts of the two compounds with three recycles of PVPB 118

Table 2-10. Amounts of matrine and oxymatrine extracted with different solvents in washing and elution steps 120

Table 2-11. Determination of bound amounts of matrine and oxymatrine with milk and egg 121

Table 2-12. Extracted amounts of the two compounds from SFA within three repetitions of PSImN 122

Table 2-13. Extracted amounts of tanshinones with different solvents in washing and elution stages 123

Table 2-14. Concentrations of the three target compounds from different commercial functional drinks 125

Table 2-15. Adsorbed amount of three target compounds on different sorbents 128

List of Figures

Figure 1-1. The molecular structure of ET-743 21

Figure 1-2. Basic theory of MIP and SPE 23

Figure 1-3. Structures of (1) quercitrin, (2) myricetin and (3) amentoflavone 30

Figure 1-4. Process of mutil-phase extraction 31

Figure 1-5. Chemical structures of four bioactive compounds. 37

Figure 1-6. Schematic illustrations of the imprint formation and molecular recognition 40

Figure 1-7. Effect of different ultrasonic and dipping times on the extracted amount 45

Figure 1-8. Effect of volumes of methanol on the extracted amount of LQ 46

Figure 1-9. Effect of different dipping time on the extraction amount of four compounds 49

Figure 1-10. Effect of different dipping temperature on the extraction amount 49

Figure 1-11. Effect of different concentration of NaOH on the extraction amount 50

Figure 1-12. Effect of component of acetone in methanol, liquid/solid ratio and their reciprocal interaction on extraction amount 55

Figure 1-13. Effect of component of dipping time, liquid/solid ratio and their reciprocal interaction on extraction amount 55

Figure 1-14. Effect of component of dipping time, component of acetone in methanol and their reciprocal interaction on extraction amount 56

Figure 1-15. Chromatograms of (a) extract sample, (b) SPE by 15% methanol aqueous solution at first step and (c) SPE by 50% methanol aqueous solution at second step 58

Figure 1-16. Chromatograms of different SPE processes (a) SPE using C18 sorbent and (b) SPE using MIP sorbent 60

Figure 1-17. Chromatographam of C. obtusa after different extraction process 62

Figure 1-18. The amount of myricetin in collected methanol with different repetitions of MPE extraction. 65

Figure 1-19. Chromatograms of dipping extraction, washing and elution stage of MPE 66

Figure 2-1. Molecular structures of four monosaccharides 88

Figure 2-2. The preparation scheme of alkyl pyridinium polymer 93

Figure 2-3. Preparation scheme for the amino-imidazolium polymer 94

Figure 2-4. Molecular structures of target compounds (A) cryptotanshinone, (B) tanshinone I, (C) tashinone IIA and template (D) 9, 10-phenanthrenequinone 96

Figure 2-5. Chemical structures of all ionic liquid-based porous polymers 97

Figure 2-6. Chemical structures of all ionic liquid-based porous polymers 98

Figure 2-7. The preparation scheme of all functionalized microsphere polymers 99

Figure 2-8. Process of multi-phase extraction 100

Figure 2-9. Effect of the amount of adsorbents on the adsorption of lactic acid 103

Figure 2-10. Proposed scheme of the anion exchange mechanism on porous polymers 105

Figure 2-11. Adsoprtion efficiency at different temperatures on three sorbents 107

Figure 2-12. Adsorbed amounts of three flavones on different sorbents 111

Figure 2-13. Chromatograms of extracts from green tea after different extraction processes 114

Figure 2-14. Chromatogram of extract from licorice 115

Figure 2-15. Chromatograms of SPE after different extraction processes 119

Figure 2-16. Chromatograms of SPE after different extraction processes 120

Figure 2-17. Chromatograms of SPE after different extraction process 124

Figure 2-18. Chromatograms of SPE separation 127

Figure 2-19. Process of multi-phase extraction 130

Figure 2-20. Chromatograms of standards, extraction with methanol and with MPE after different extraction process 132

초록보기

 천연식물에서 중요한 화합물 중 하나는 생리활성성분으로 매우 적은양으로 존재한다. 천연식물의 전통적인 응용 방법 (제분, 삶기 또는 증발)의 효율은 아주 낮다. 천연 식물의 치료상의 효율을 증가시키기 위해서 생리활성성분은 추출, 농축되고 정제되어야 한다.

◆ 추출조건은 최적화 되었다. 또한 추출 효율은 수학적 방법을 사용하여 증가하였다.

◆ SPE 기술로 다른 생리활성 성분의 정제와 SPE 를 기반으로한 새로운 방법은 효율을 증가시키기 위해 개발되었다.

◆ 다공성 폴리머의 작용기가 이온성 액체로 치환되어 수정되었다. 수정된 다공성 폴리머 흡착제와 생리활성 성분 사이의 상호작용이 증가되었다.

◆ 새로운 방법에서 일반적인 흡착제의 응용은 분리 효율을 증가시켰다. 그리고 새로운 흡착제와 방법은 생리활성 성분의 분리에 많은 장점을 가지는 것으로 확인 되었다.

결론적으로, 추출과 분리 효율을 증가시킬 필요가 있으며, 모든 과정에서 유기용매의 양을 감소시킬 필요가 있다. 이 후 연구에서,

1. 다른 방법으로 일반적인 재료를 확인하고, 추출 효율을 증가 시키기 위해 새로운 방법을 개발 할 것이다.

2. 생리활성 성분의 분리 재료는 분리와 흡착 효율을 증가시킬 필요가있다. 또한 모든 과정에서 유기용매를 감소시킬 필요가 있다.

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기