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Abstract 13

제1장 서론 15

제2장 문헌조사 17

2.1. 하천복원의 의미 17

2.2. 해수천의 의미 17

2.3. 하천복원기술 및 국내외 사례 18

2.3.1. 하천복원기술 18

2.3.2. 국내사례 20

2.3.3. 국외사례 23

2.4. BM의 일반적인 특징 28

제3장 실험장치 및 방법 30

3.1. 연구목표 및 범위 30

3.2. 연구방법 32

3.3. 분석 방법 34

3.4. 현장투여 미생물제제(BM S-2)의 배양액 준비 및 투여 방법 35

제4장 연구결과 및 고찰 36

4.1. 유황 조사 36

4.1.1. 구간별 간격 및 수심 36

4.1.2. 유속 37

4.2. 환경 조건 38

4.2.1. 수온 38

4.2.2. 염분(Salinity) 39

4.2.3. 수소이온농도(pH) 39

4.2.4. 용존산소(DO) 40

4.2.5. 산화환원전위(ORP) 42

4.3. 수질 항목 43

4.3.1. 화학적산소요구량(COD) 43

4.3.2. 부유물질(SS) 44

4.3.3. 총질소(T-N) 45

4.3.4. 질소의 물질수지(2013년 11월 2일) 46

4.3.5. 총인(T-P) 47

4.3.6. 인의 물질수지(2013년 11월 2일) 48

4.3.7. Chl-a 49

4.4. 저질 항목 50

4.4.1. 저질 화학적산소요구량(COD) 50

4.4.2. 강열감량 51

4.5. 현장 사진 52

4.5.1. 2012년 7월 4일 52

4.5.2. 2012년 9월 27일 52

4.5.3. 2012년 10월 31일 53

4.5.4. 2012년 11월 21일 54

4.5.5. 2013년 03월 03일 이후 54

4.6. 해수천 BM-S-1처리 전후의 미생물군집변화 분석 55

4.6.1. Pyrosequencing 기법에 의한 미생물 군집의 변화 비교 (처리 후 약 1개월 경과) 56

4.6.2. Pyrosequencing 기법에 의한 미생물 군집의 변화 비교 (처리 후 약 8개월 후) 70

4.6.3. Pyrosequencing 기법에 의한 미생물 군집의 변화 비교 (처리 후 약 11개월 후) 74

제5장 결론 79

참고문헌 80

표목차

〈Table 2.1〉 Putative functions of the Microbial community from the consortium, BM-S-1 29

〈Table 3.1〉 The test criteria to water quality of the stream 31

〈Table 3.2〉 The location of sampling sites of the stream 33

〈Table 3.3〉 The sampling date and analysis items 33

〈Table 3.4〉 The test criteria 34

〈Table 3.5〉 Analytical methods for samples from water and sediment 35

그림목차

〈Fig. 2.1〉 The view of Jeonjucheon, Jeonju, South Korea 21

〈Fig. 2.2〉 The view of Onchoncheon, Busan, South Korea 22

〈Fig. 2.3〉 The natural education site of Onchoncheon, Busan, South Korea 22

〈Fig. 2.4〉 The restoration of Gwanchicheon, Jeonbuk, South Korea 22

〈Fig. 2.5〉 View of Alterbach River before restoration 23

〈Fig. 2.6〉 View of Alterbach River after restoration 23

〈Fig. 2.7〉 Hotophia Creek before treatment 24

〈Fig. 2.8〉 Hotophia Creek before treatment 2 years after treatment 24

〈Fig. 2.9〉 View of Trinity River(california, USA) after restoration 26

〈Fig. 2.10〉 Enka River before(left) and after(right) restoration 27

〈Fig. 2.11〉 The water-front area of river side park in Enka River 27

〈Fig. 2.12〉 Enka River before(left) and after(right) restoration 27

〈Fig. 2.13〉 Microbial community composition of a microbial consortium, BM-S-1 28

〈Fig. 3.1〉 View of the constructed sea stream at Dongsamdong, Busan, South Korea and location of drains of run-off water 31

〈Fig. 3.2〉 View of one of the typical drains at the stream 31

〈Fig. 3.3〉 View of sites for water and sediment quality of the stream 32

〈Fig. 3.4〉 Sampling of water and sediment for analysis 34

〈Fig. 4.1〉 The investigation site of water and sediment of the constructed sea stream at Dongsamdong, Busan, South Korea 36

〈Fig. 4.2〉 The interval and water level of the stream 37

〈Fig. 4.3〉 The water temperature changes at the test sites of the stream during the test period 38

〈Fig. 4.4〉 The salinity changes at the test sites of the stream during the test period 39

〈Fig. 4.5〉 The pH changes at the test sites of the stream during the test period 40

〈Fig. 4.6〉 The DO changes at the test sites of the stream during the test period 41

〈Fig. 4.7〉 The ORP changes at the test sites of the stream during the test period 42

〈Fig. 4.8〉 The COD changes at the test sites of the stream during the test period 43

〈Fig. 4.9〉 The SS changes at the test sites of the stream during the test period 44

〈Fig. 4.10〉 The T-N changes at the test sites of the stream during the test period 45

〈Fig. 4.11〉 The mass balance of nitrogen at the test sites of the stream during the test period 46

〈Fig. 4.12〉 The T-P changes at the test sites of the stream during the test period 47

〈Fig. 4.13〉 The mass balance of phosphorus at the test sites of the stream during the test period 48

〈Fig. 4.14〉 The Chl-a changes at the test sites of the stream during the test period 49

〈Fig. 4.15〉 The COD of sediment changes at the test sites of the stream during the test period 50

〈Fig. 4.16〉 The ignition loss changes at the test sites of the stream during the test period 51

〈Fig. 4.17〉 View of the stream 85days before BM-S-1 treatment 52

〈Fig. 4.18〉 View of the stream one day before BM-S-1 treatment 53

〈Fig. 4.19〉 View of the stream 33 days after BM-S-1 treatment 53

〈Fig. 4.20〉 View of the stream 54 days after BM-S-1 treatment 54

〈Fig. 4.21〉 View of the stream 158 days after BM-S-1 treatment 55

〈Fig. 4.22〉 View of the stream 295 days after BM-S-1 treatment 55

〈Fig. 4.23〉 View of the stream 401 days after BM-S-1 treatment 55

〈Fig. 4.24〉 The cluster analysis of the test sites based on UNI-FRAC analysis of microbial communities (before... 56

〈Fig. 4.25〉 The cluster analysis of the test sites based on UNI-FRAC analysis of microbial communities (one month after BM S-1 treatment) 57

〈Fig. 4.26〉 The combined cluster analysis of the test sites based on UNI-FRAC analysis of microbial communities 58

〈Fig. 4.27〉 Distribution of the dominant microbial phylum before treatment 60

〈Fig. 4.28〉 Distribution of the dominant microbial phylum one month after treatment 61

〈Fig. 4.29〉 Distribution of the dominant microbial genus before treatment 62

〈Fig. 4.30〉 Distribution of the dominant microbial genus one month after treatment 63

〈Fig. 4.31〉 Distribution of the dominant microbial species before treatment 64

〈Fig. 4.32〉 Distribution of the dominant microbial species one month after treatment 65

〈Fig. 4.33〉 Changes of the dominant microbial communities in the Site 3 before and one month after treatment 66

〈Fig. 4.34〉 The distribution percentage of Psychrilyobacter atlanticus for each site (before treatment of BM-S-1) 67

〈Fig. 4.35〉 The distribution percentage of Psychrilyobacter atlanticus for each site (after treatment of BM-S-1) 67

〈Fig. 4.36〉 The distribution percentage of Desulfuromonadaceae_uc_s for each site (before treatment of... 68

〈Fig. 4.37〉 The distribution percentage of Desulfuromonadaceae_uc_s for each site (after treatment of... 68

〈Fig. 4.38〉 The distribution percentage of Sulfurimonas_uc for each site (before treatment of BM-S-1) 69

〈Fig. 4.39〉 The distribution percentage of Sulfurimonas_uc for each site (after treatment of BM-S-1) 69

〈Fig. 4.40〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (St. 2) 70

〈Fig. 4.41〉 Changes of the dominant microbial communities before and after treatment of BM-S-1 (Site 2) 71

〈Fig. 4.42〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (Site 3) 71

〈Fig. 4.43〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (continuing Site 3) 72

〈Fig. 4.44〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (continuing Site 3) 72

〈Fig. 4.45〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (continuing Site 3) 73

〈Fig. 4.46〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313) treatment of BM-S-1 (St. 4) 73

〈Fig. 4.47〉 The cluster analysis of all test sites before(09-28-12) and after (05-23-13) BM-S-1 treatment based on UNI-FRAC analysis of microbial communities 74

〈Fig. 4.48〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313, 082613) treatment of BM-S-1 (St. 2) 75

〈Fig. 4.49〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313, 082613) treatment of BM-S-1 (Site 1) 75

〈Fig. 4.50〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313, 082613) treatment of BM-S-1 (Site 2) 76

〈Fig. 4.51〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313, 082613) treatment of BM-S-1 (Site 3) 76

〈Fig. 4.52〉 Changes of the dominant microbial communities before(092812) and after(103112, 052313, 082613) treatment of BM-S-1 (St. 4) 77

〈Fig. 4.53〉 The cluster analysis of all test sites before(092812) and after(103112, 052313, 082613) BM-S-1 treatment based on UNI-FRAC analysis... 78