본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

목차보기

표제지

목차

I. 서론 11

II. 재료 및 방법 15

1. 실험재료 15

1) 시료 15

2) 시료 추출 및 분획 15

3) 시약 및 기기 18

2. 실험 방법 21

1) 총 폴리페놀 함량 측정 21

2) 항산화 효과 측정 22

3) 주름억제 효과 측정 24

4) 항균 효과 측정 29

5) Multiple emulsion 제조 및 실험 30

6) 작약 추출물을 함유한 Multiple emulsion의 안정성 측정 39

7) 작약 추출물을 함유한 Multiple emulsion의 안전성 측정 46

8) 통계처리 47

III. 결과 및 고찰 48

1. 작약 추출물 및 분획물의 수율 48

2. 총 폴리페놀 함량 50

3. 항산화 효과 측정 결과 51

1) 전자공여능 효과 51

2) ABTS radical cation decolorization 효과 55

4. 주름억제 효과 확인 59

1) Collagenase inhibition 효과 59

2) Elastase inhibition 효과 61

3) Western blot을 통한 MMP 단백질 발현 효과 63

5. 항균 효과 결과 71

6. Multiple emulsion 제조 및 실험결과 확인 83

1) ARACHIDYL BEHENYL ALCOHOL (AND) ARACHIDYL GLUCOSIDE 함량실험 결과 83

2) CETEARYL ALCOHOL (AND) CETEARYL GLUCOSIDE 함량 실험 결과 85

3) Glycolipid surfactant 함량 실험 결과 87

4) SODIUM N-STEAROYL-L-GLUTAMATE 함량 실험결과 89

5) Total surfactants 함량 실험 결과 91

6) Total oil 함량 실험 결과 93

7) CETYL ALCOHOL 함량 실험 결과 95

7. 작약 추출물을 함유한 Multiple emulsion 안정성 측정 결과 97

1) 작약 추출물을 함유한 Multiple emulsion 제조 결과 97

2) pH 측정 결과 99

3) 점도 측정 결과 101

4) 온도에 따른 안정성 측정 결과 103

5) 온도순환(Cycle chamber)에 따른 특수·가혹 보존 시험측정 결과 104

6) 광 안정성 시험 측정 결과 106

8. 작약 추출물을 함유한 Multiple emulsion 안전성 측정 결과 107

1) 첩포 시험 결과 107

IV. 결론 109

V. 참고문헌 113

초록 119

Abstract 120

List of Tables

Table 1. The experimental quantity variation formulation(A~D) of the ARACHIDYL BEHENYL ALCOHOL (AND) ARACHIDYL GLUCOSIDE contents variation. 32

Table 2. The experimental quantity variation formulation(E~H) of the CETEARYL ALCOHOL (AND) CETEARYL GLUCOSIDE contents variation. 33

Table 3. The experimental quantity variation formulation(I~L) of the glycolipid surfactant contents variation. 34

Table 4. The experimental quantity variation formulation(M~R) of the SODIUM N-STEAROYL-L-GLUTAMATE contents variation. 35

Table 5. The experimental multiple emulsion formulation(S~Y) of the total surfactants contents variation. 36

Table 6. The experimental quantity variation formulation(AA~AE) of the total oil contents variation. 37

Table 7. The experimental quantity variation formulation(AF~AH) of the CETANOL contents variation. 38

Table 8. The experimental formulation of the multiple emulsion containing Paeonia lactiflora extracts. 41

Table 9. Check points in stability test of emulsion containing in PLE constant temperature condition (0, 25, 45℃) 43

Table 10. Recording of Patch test reactions according to International Contact Dermatitis Research Group (ICDRG) Criteria. 46

Table 11. Yields of PLE extracts and PLE fractions. 49

Table 12. The contents of total polyphenol from PLE. 50

Table 13. Antimicrobial activity of PLE 74

Table 14. Antimicrobial activity of PLE-D 76

Table 15. Antimicrobial activity of PLE-E 78

Table 16. Antimicrobial activity of PLE-B 80

Table 17. Antimicrobial activity of PLE-W 82

Table 18. Results of stability test of AI in the constant-temperature incubator. 103

Table 19. Results of stability test of AI in the cycling chamber. 105

Table 20. Results of stability test of AI on daylight condition. 106

Table 21. Results of Human patch test on AI. 108

List of Figures

Fig. 1. The procedure for extraction from Paeonia lactiflora. 16

Fig. 2. The procedure for fraction from Paeonia lactiflora extracts. 17

Fig. 3. Preparation process of multiple emulsion in this experiments 31

Fig. 4. Preparation process of multiple emulsion containing Paeonia lactiflora extracts in this experiments. 40

Fig. 5. Cycle temperature change with day for 1 cycle. 44

Fig. 6. Electron donating ability of Electron donating ability of PLE. 53

Fig. 7. Electron donating ability of fractions from PLE. 54

Fig. 8. ABTS Free radical scavenging activity of PLE. 57

Fig. 9. ABTS Free radical scavenging activity of fractions from PLE. 58

Fig. 10. Collagenase inhibition activity of PLE. 60

Fig. 11. Elastase inhibition activity of PLE. 62

Fig. 12. Cell viability of PLE on HaCaT cell. 65

Fig. 13. Cell viability of fractions from PLE on HaCaT cell. 66

Fig. 14. Cell viability of PLE on Hs68 cell. 67

Fig. 15. MMP protein expression condition by UV-B on Hs68 cell. 69

Fig. 16. MMP13 protein expression inhibition by UV-B on Hs68 cell. 70

Fig. 17. The antimicrobial activity of PLE on microbial by disc diffusion method, respectively. 73

Fig. 18. The antimicrobial activity of PLE-D on microbial by disc diffusion method, respectively. 75

Fig. 19. The antimicrobial activity of PLE-E on microbial by disc diffusion method, respectively. 77

Fig. 20. The antimicrobial activity of PLE-B on microbial by disc diffusion method, respectively. 79

Fig. 21. The antimicrobial activity of PLE-W on microbial by disc diffusion method, respectively. 81

Fig. 22. Optical microscope images of multiple emulsion formulation A~D(x800) 84

Fig. 23. Optical microscope images of multiple emulsion formulation E~H(x800) 86

Fig. 24. Optical microscope images of multiple emulsion formulation I~L(x800) 88

Fig. 25. Optical microscope images of multiple emulsion formulation M~R(x800) 90

Fig. 26. Optical microscope images of multiple emulsion formulation S~Y(x800) 92

Fig. 27. Optical microscope images of multiple emulsion formulation AA~AE(x800) 94

Fig. 28. Optical microscope images of multiple emulsion formulation AF~AH(x800) 96

Fig. 29. Optical microscope images of multiple emulsion formulation AI(x800) 98

Fig. 30. Measurement of pH on the AI in the constant-temperature incubator. 100

Fig. 31. Measurement of viscosity on the AI in the constant-temperature incubator. 102

Fig. 32. Photograph of human patch test of AI. 108

Fig. 33. The mechanisms modulating the protective effect of Paeonia lactiflora extracts against UV-B 111

Fig. 34. The multiple emulsion diagram of surfactant and oil contents variation. 112

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기