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Title Page

Contents

I. INTRODUCTION 6

II. MATERIALS AND METHODS 10

Virus rescue and cells 10

Polymorphism at the human H1 HA residue 222 11

Growth kinetics 11

Cell binding assay 11

Ethics of animal experiments 13

Body weight changes of infected mice 13

Virus replication in the lungs of infected mice 13

III. RESULTS 14

HA residue 222 and its polymorphism 14

Growth and cell binding properties of the HA D222G mutant 15

Effect of the HA D222G substitution on K/09 pathogenesis 16

IV. DISCUSSION 18

V. References 23

ABSTRACT 36

국문초록 37

List of Tables

Table 1. Pathologic findings of rK/09 and rK/HA:D222G viruses in BALB/c mice 35

List of Figures

Figure 1. Position of HA residue 222 and its polymorphisms in human H1 HAs 31

Figure 2. Growth and cell binding properties of the HA D222G mutant 32

Figure 3. Body weight changes of infected mice 34