본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

목차보기

Title Page

GENERAL ABSTRACT

Contents

LIST OF ABBREVIATIONS 12

GENERAL INTRODUCTION 13

REFERENCES 15

CHAPTER I. Evidence of genome duplication revealed by sequence analysis of multi-loci expressed sequence tag-simple sequence repeat bands in Panax ginseng Meyer 18

ABSTACT 19

INTRODUCTION 20

MATERIAL AND METHODS 22

Sample preparation and preliminary PCR 22

Sequencing of each individual band among multiple bands 22

Sequence analysis and design of locus-specific primer for gm47n marker 23

RESULTS 24

Separation and re-amplification of individual bands 24

Sequence comparison of Band-A and Band-B 26

Locus-specific PCR amplification for the gm47n marker 30

DISCUSSION 33

PCR amplification of two paralogous loci derived from genome duplication in P. ginseng 33

Sequence variation between paralogous bands 34

Additional artifact bands are caused by coexistence of two paralogous bands 34

Development of locus-specific markers and future application 35

REFERENCES 37

CHAPTER II. Micro-collinearity within Panax ginseng genome sequences resulted from two rounds of whole genome duplication 41

ABSTRACT 42

INTRODUCTION 44

MATERIAL AND METHODS 46

Plant material and extraction of DNA and RNA 46

Genome sequencing, assembly and repeat masking 47

Transcriptome sequencing and assembly 47

Selection of homoeologous scaffolds and comparative analysis 48

Gene annotation and expression profiling of homoeologous scaffolds 48

Development of STS markers in micro-collinearity blocks 49

RESULTS 51

Genome sequence and transcriptome assembly 51

Identification of homoeologous scaffolds from draft sequence 54

Annotation of the homoeologous scaffold sequences 58

Comparative analysis of the homoeologous scaffold sequences 60

Paralogous gene pairs and its divergence in four paralogous blocks 71

Expression profiles of paralogous gene pairs 74

Designing locus-specific SNP markers 78

DICUSSIONS 81

REFERENCES 87

APPENDIX 93

국문초록 104

List of Tables

Table 1-1. Summary of variation among EST and amplified sequences 29

Table 2-1. Statistics of P.ginseng draft genome sequence 53

Table 2-2. Information of eight scaffolds used in this study 57

Table 2-3. Distribution of repeats among four blocks. 59

Table 2-4. Annotation of coding genes and paralogous pairs among four blocks. 66

Table 2-5. Median Ks and Ka value between paralogous genes 73

Table 2-6. FPKM values of six tissues of nine genes shared in paleo duplicated relationship 75

Table 2-7. Primer information of SNP marker between Chunpoong and... 79

List of Figures

Figure 1-1. Band profiles of the gm47n SSR marker using genomic DNA and... 25

Figure 1-2. Multiple sequence alignment of eluted bands derived from different... 28

Figure 1-3. PCR amplification with the gm47n Band-B locus-specific primer. 31

Figure 1-4. Application of locus-specific primer of the gm47n marker into F2... 32

Figure 2-1. Phylogenetic analysis of 10 putative genes encoding CYP716A47... 56

Figure 2-2. Mimetic diagram of "zig-zag" extension of scaffolds using dot-plot... 63

Figure 2-3. Comparative analysis of four homoeologous blocks in ginseng. 65

Figure 2-4. Micro-collinearity of four blocks and frequency distribution of Ks... 72

Figure 2-5. Digital expression profiles and clustering of nine homeologous gene... 77

Figure 2-6. Development of STS markers and application to F₂ population. 80

Figure 2-7. Evolution model of the ginseng genome and its relatives. 86

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기