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표제지 2

목차 4

Ⅰ. 서론 13

1. 연구배경 및 필요성 13

1.1. 연구대상소재 13

1.2. 대마 줄기 21

1.3. 기능성 화장품 25

1.4. 목표 기능성 28

2. 연구목적 39

Ⅱ. 재료 및 방법 40

1. 실험재료 40

1.1. 시료 40

1.2. 시약 및 기기 40

2. 실험방법 42

2.1. 추출 및 추출수율 측정 42

2.2. 기능성분 함량 분석 44

2.3. 항산화 활성 평가 45

2.4. 피부미용 관련 In vitro 효능검증 46

2.5. 효능지표물질 탐색 64

2.6. 통계분석 67

Ⅲ. 결과 및 고찰 68

1. 추출수율 및 총 폴리페놀 함량 68

2. 피부미용 기능성 선별 평가 70

2.1. 항산화 활성 평가 70

2.2. 항염증 효능 예비 평가: Nitric oxide (NO) 생성 억제능 측정 74

2.3. 피부 미백 효능 예비 평가: Melanin 생성 억제능 측정 76

2.4. 주름 개선 효능 예비 평가: 피부세포재생능 측정 78

2.5. 아토피 개선 효능 예비 평가: β-hexosaminidase release 측정 80

3. 피부미용 효능평가(In vitro) 82

3.1. 항염증 효능검증 82

3.2. 피부 미백 효능검증 94

3.3. 아토피 개선 효능검증 99

4. 효능지표물질 탐색 105

4.1. HPLC를 이용한 Flavonoids의 정량적 분석 105

4.2. 항염증 효능평가 108

4.3. 피부 미백 효능평가 110

4.4. 아토피 개선 효능평가 112

4.5. 효능지표물질 탐색 114

Ⅳ. 요약 및 결론 116

1. 대마 줄기 추출물의 기능성분 측정 및 피부미용 기능성 선정을 위한 스크리닝 실험 116

2. 대마 줄기 70% 에탄올 추출물의 효능평가 117

3. 대마 줄기 70% 에탄올 추출물의 지표물질 탐색 118

Ⅴ. 참고문헌 120

Abstract 135

표목차 9

Table 1. The activity of hemp stem used in the previous study 24

Table 2. Anti-inflammatory antibody used in western blot analysis 49

Table 3. Anti-inflammatory primer sequences used for real-time PCR 51

Table 4. Whitening antibody used in western blot analysis 54

Table 5. Whitening primer sequences used for real-time PCR 56

Table 6. Atopy improvement antibody used in western blot analysis 61

Table 7. Atopy improvement primer sequences used for real-time PCR 63

Table 8. HPLC condition of flavonoids in the HPLC analysis 65

Table 9. The list of standard product 66

Table 10. Extracted yield and total polyphenol content and total flavonoid... 69

Table 11. Effect of hemp stem extracts on ABTS radical scavenging activity 72

Table 12. Effect of hemp stem extracts on DPPH radical scavenging activity 73

Table 13. Flavonoids contents in hemp stem 70% ethanol extract 107

Table 14. IC₅₀ values of hemp stem extract and flavonoid compounds in... 115

그림목차 10

Fig. 1. Uses of Hemp for different purposes 20

Fig. 2. Mechanism of inflammation in macrophage 32

Fig. 3. Pathway of melanin synthesis 35

Fig. 4. Mechanism of atopic dermatitis 38

Fig. 5. The procedure for the extraction of hemp stem 43

Fig. 6. Effect of hemp stem extracts on (A) cell viability and (B) NO product... 75

Fig. 7. Effect of hemp stem extracts on (A) cell viability and (B) Melanin... 77

Fig. 8. Effect of hemp stem extracts on (A) cell viability and (B) skin... 79

Fig. 9. Effect of hemp stem extracts on (A) cell viability and (B) β... 81

Fig. 10. Effect of hemp stem 70% ethanol extracts on (A) cell viability and... 83

Fig. 11. Inhibitory effects of hemp stem 70% ethanol extracts on ROS... 85

Fig. 12. Effect of hemp stem 70% ethanol extracts on protein expressions of... 87

Fig. 13. Effect of hemp stem 70% ethanol extracts on mRNA expressions of... 88

Fig. 14. Effect of hemp stem 70% ethanol extracts on (A) NF-κB activation... 90

Fig. 15. Effect of hemp stem 70% ethanol extracts on mRNA expressions of... 91

Fig. 16. Effect of hemp stem 70% ethanol extracts on (A) MAPK activation... 93

Fig. 17. Effect of hemp stem 70% ethanol extracts on (A) cell viability and... 95

Fig. 18. Effect of hemp stem 70% ethanol extracts on (A) tyrosinase, (B)... 97

Fig. 19. Effect of hemp stem 70% ethanol extracts on (A) tyrosinase, (B)... 98

Fig. 20. Effect of hemp stem 70% ethanol extracts on (A) cell viability and... 100

Fig. 21. Effect of hemp stem 70% ethanol extracts on (A) MAPK activation... 102

Fig. 22. Effect of hemp stem 70% ethanol extracts on mRNA expressions of... 104

Fig. 23. The chromatogram of HPLC flavonoids analysis 106

Fig. 24. Effect of flavonoids on (A) cell viability and (B) NO product levels in... 109

Fig. 25. Effect of flavonoids on (A) cell viability and (B) melanin contents in... 111

Fig. 26. Effect of flavonoids on (A) cell viability and (B) β-hexosaminidase... 113

초록보기

 Hemp stems, among the by-products of hemp, are relatively more likely to be discarded and have primarily been used as industrial materials in construction, textiles, and paper production. However, recent reports of significant bioactivity related to skin health have drawn attention to the potential applications of hemp stems in the field of cosmetics. Despite this, research on the skin-related efficacy of hemp stems remains limited compared to other parts of the plant, particularly in terms of elucidating mechanisms of action and identifying key bioactive markers. Therefore, this study aimed to propose utilization strategies for hemp stem resources by verifying their skin-related functionalities. Anti-inflammatory, skin-whitening, and atopic dermatitis improvement effects were evaluated using both 70% ethanol and hot water extracts of hemp stems. The 70% ethanol extract exhibited superior inhibitory effects in these assays; however, no significant results were observed in the wrinkle improvement test. The 70% ethanol extract of hemp stems showed excellent efficacy in anti-inflammatory, whitening, and anti-atopic evaluations, along with significant inhibition of related key proteins and gene expressions. Furthermore, among the major flavonoids identified in hemp stems—caffeic acid, rutin, p-coumaric acid, ferulic acid, quercetin, apigenin, and kaempferol—each compound was analyzed and individually tested for skin-related efficacy. Apigenin showed the most potent NO production inhibitory effect, kaempferol demonstrated the highest melanin synthesis inhibition, and quercetin showed strong β-hexosaminidase inhibitory activity. These findings provide foundational data for the development of cosmetic products using natural resources that meet consumer needs and demonstrate the potential of hemp stems as functional ingredients for skin care applications.

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