본문 바로가기 주메뉴 바로가기
22대 대한민국 국회 국회정보길라잡이 국회의원검색
회원가입 로그인
HOME

정보검색

소장정보 검색

국회도서관 홈으로 정보검색 소장정보 검색

결과 내 검색

동의어 포함

고급검색

인명/단체명

저자정보 상세정보
인명/단체명을 입력하세요.

키워드

  • 대표어

  • 외국어

-

목차보기

표제지 2

목차 5

Part Ⅰ. 1,3 - Diphenylisobenzofuran을 이용한 일중항 산소 검출 민감도 향상 및 Type II 감광활성 평가에 응용 14

1. 서론 15

2. 재료 및 방법 18

2.1. 실험재료 18

2.2. Light emitting diode 조사 장치 18

2.3. DPBF 및 감광제의 흡광 및 형광 특성 18

2.4. DPBF의 광안정성 19

2.5. 감광활성에 의해 생성된 일중항 산소 검출 19

2.6. 통계처리 20

3. 결과 및 고찰 24

3.1. 다양한 광원에서 DPBF의 안정성 평가 24

3.2. DPBF를 이용해 빛 조사 하에서 감광제에 의해 형성되는 일중항 산소 검출 환경 조성 29

3.3. DPBF를 이용한 RF와 유도체의 일중항 산소 생성 검출 33

4. 결론 51

Part Ⅱ. 비산소성 기질 접촉 의존 메커니즘에 따른 커큐민의 감광활성 규명 53

1. 서론 54

2. 재료 및 방법 57

2.1. 실험재료 57

2.2. Light emitting diode 조사 장치 57

2.3. 세포 주 및 세포 배양 57

2.4. 진공 환경에서 감광활성 평가 58

2.5. ROS (Reactive Oxygen Species) 생성 수준 평가 58

2.6. 지질과산화물 생성량 측정 60

2.7. Free fatty acid에 따른 용액의 pH 측정 61

2.8. 광안정성 평가 61

2.9. Zeta 입자 크기 측정 62

2.10. 통계처리 62

3. 결과 및 고찰 66

3.1. 산소와의 상호작용을 통한 커큐민 감광활성 분석 66

3.2. 유리지방산 함유 Oil - in - Water (O/W) 시스템에서의 광활성 동태 분석 77

3.3. 식용유지 함유 O/W system에서 커큐민에 의한 광산화 유도 96

4. 결론 106

Part Ⅲ. BSA가 커큐민의 감광활성에 미치는 영향과 염증 조절을 위한 광역학적 활용 108

1. 서론 109

2. 재료 및 방법 111

2.1. 실험재료 111

2.2. Light emitting diode 조사 장치 112

2.3. 세포 주 및 세포 배양 112

2.4. 커큐민과 BSA 상호작용의 분광학적 평가 112

2.5. H₂O₂ 생성량 평가 113

2.6. 지질과산화물 생성량 측정 113

2.7. 산화 전위 측정 114

2.8. 세포독성 평가 115

2.9. NO 분비량 측정 115

2.10. Western blot 116

2.11. qRT - PCR 117

2.12. 세포 내·외 ROS 측정 117

2.13. 통계처리 118

3. 결과 및 고찰 120

3.1. BSA에 의한 커큐민의 감광활성 강화 120

3.2. 커큐민의 감광활성에 의한 염증조절활성 평가 137

4. 결론 146

참고문헌 148

ABSTRACT 156

표목차 13

Table 1. Concentrations of emulsifiers used in the O/W system... 79

Table 2. The PCR primers used for detecting inflammatory... 119

Table 3. Electrochemical properties of curcumin and curcumin -... 136

그림목차 9

Fig. 1. The emission spectra of different light sources including... 22

Fig. 2. A reaction scheme of 1,3 - diphenylisobenzofuran (DPBF)... 23

Fig. 3. Changes in color and fluorescence intensities of DPBF under... 28

Fig. 4. Effects of rose bengal (RB) on color and fluorescence... 32

Fig. 5. Changes in color intensities of DPBF by different... 38

Fig. 6. Absorbance (A) and emission fluorescence excited at 350... 39

Fig. 7. Changes in emission fluorescence intensities of DPBF by... 42

Fig. 8. Comparison of singlet oxygen level generated by different... 45

Fig. 9. Comparison of singlet oxygen level generated by different... 48

Fig. 10. Comparison of DPBF absorbance and fluorescence based... 50

Fig. 11. Jablonski diagram illustrating the photosensitization... 63

Fig. 12. Structure of curcumin used in this study (A), spectra of... 65

Fig. 13. Comparison of the photosensitizing properties of... 68

Fig. 14. Evaluation of singlet oxygen generation by curcumin and... 72

Fig. 15. Evaluation of singlet oxygen generation by curcumin and... 74

Fig. 16. Evaluation of ROS generation by curcumin and... 75

Fig. 17. Generation of H₂O₂ by curcumin and riboflavin under... 76

Fig. 18. Induction of lipid peroxidation by curcumin in the O/W... 82

Fig. 19. Effects of curcumin on lipid peroxidation induced by... 84

Fig. 20. Change in the emulsion droplet size with linoleic acid... 86

Fig. 21. Photostability of curcumin in O/W system under the... 89

Fig. 22. A correlation between curcumin photodegradation and... 90

Fig. 23. Photostability of curcumin in O/W system under the... 95

Fig. 24. Induction of lipid peroxidation by curcumin in the O/W... 100

Fig. 25. Induction of lipid peroxidation by curcumin in the O/W... 103

Fig. 26. Change in an emulsion droplet size by different... 105

Fig. 27. Changes in spectra of absorbance (A) and emission... 123

Fig. 28. Spectra of emission fluorescence of BSA (0 - 1%) only... 124

Fig. 29. Changes in photostability of curcumin by BSA and... 127

Fig. 30. Effects of BSA on H₂O₂ generation by curcumin under... 131

Fig. 31. Effect of BSA on lipid peroxidation induced by curcumin... 132

Fig. 32. Cyclic voltammograms, spectra of absorbance and... 135

Fig. 33. Modulatory effect of blue LED irradiation on cell viability... 141

Fig. 34. Effect of curcumin and blue LED irradiation on th... 142

Fig. 35. Effect of curcumin and blue LED irradiation on mRNA... 144

Fig. 36. Changes in ROS level of RAW264.7 by curcumin and blue... 145

초록보기

 Singlet oxygen is a type of reactive oxygen species and generated from the reaction of Type II photosensitization. Since 1,3-Diphenylisobenzofuran (DPBF) is used as a chromogenic probe with a peak absorbance at 410 nm to detect singlet oxygen, there is severe interference with photosensitizing pigments through absorption of blue light. In this study, the level of singlet oxygen was quantified through the analysis of the decrease in DPBF fluorescence, a indicative of the reaction with singlet oxygen. The fluorescence-based analysis was more sensitive and accurate than the one based on absorbance measurement. The fluorescence detection of singlet oxygen levels generated by various photosensitizers, including riboflavin, flavin mononucleotide, flavin adenine dinucleotide, lumichrome and lumiflavin, under blue light irradiation also permitted more effective measurement of their activity without interference from color. The results suggest that singlet oxygen detection based on the fluorescence of DPBF is a more effective approach than colorimetric analysis, and it can be utilized to assess the Type II photosensitizing property of diverse compounds.

Curcumin exhibits both photosensitizing and antioxidant activities, suggesting a dualistic behavior that may involve a non-classical mechanism distinct from conventional photosensitizers. 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan assays under vacuum confirmed that curcumin's photosensitizing activity is oxygen - independent. Moreover, 9,10-Anthracenediyl-bis (methylene dimalonic acid), DPBF, 2',7'-Dichlorodihydrofluorescein and ferrous oxidation - xylenol orangebassays showed no detectable ROS generation upon light exposure, indicating a oxygen - independent photosensitivity. In linoleic acid oil in water (O/W) systems, a biphasic pattern was observed depending on emulsifier concentration, with increased photosensitivity below the critical micelle concentration and suppression above it, suggesting contact-dependent lipid interaction. A strong correlation between curcumin decolorization and lipid hydroperoxide formation indicates that lipid oxidation is accompanied by chromophore disruption, consistent with a Type I photosensitization pathway. But sodium linoleate formed self-micelles, and lipid peroxidation was not induced. In edible oil O/W models, lipid oxidation was more pronounced at low emulsifier concentrations, supporting the role of direct lipid contact in curcumin's activity. Collectively, these results identify curcumin as oxygen - independent, contact-dependent photosensitizer, offering a novel perspective on photosensitization mechanisms.

To further investigate this mechanism and to enhance its photosensitizing activity, the interaction of curcumin with bovine serum albumin (BSA), a protein with electron-rich residues that create an electron-donating environment, were investigated. A complex formation between curcumin and BSA was confirmed by the observation of a decrease in the fluorescence of BSA and an increase of light absorption and the fluorescence of curcumin. The photostability of curcumin decreased with the concentration of BSA, while the fluorescence of BSA was not affected under blue LED. Curcumin did not produce H₂O₂ under the blue LED； the level of H₂O₂ by photosensitized curcumin increased in a BSA concentration-dependent manner. The curcumin-BSA complex exhibited a lower oxidation potential than free curcumin, indicating an enhanced ability to act as an electron donor. The induction of lipid peroxidation by blue LED-sensitized curcumin was also enhanced with increasing BSA concentrations. These findings indicate that the electron-rich environment provided by BSA enhances curcumin's photosensitizing activity and protein-assisted photodynamic therapy could be a novel approach for improving the performance of a Type I photosensitizer.

In addition, the photosensitizing activity of curcumin was investigated for its potential application in photodynamic therapy (PDT) aimed at regulating inflammation. Light-activated curcumin was more effective at reducing the production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS) - stimulated macrophages. The present results suggest that photoactivation could enhance the anti-inflammatory potential of curcumin.

추천서가 (다양한 추천 자료를 만나보세요)

권호기사보기

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 기사목차

권호

기사명 원문보기 기사목차
닫기