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Since somatic embryogenesis combined with ethylmethane sulfonate (EMS) treatments is the most efficient technique for mutagenesis,

the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and

preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers

of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in

the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS

for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration,

whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not

influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher

concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six

hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with

embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean

variants were identified in the M₁R0 and M₂R₁ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration

from EMS-treated immature embryo cultures.

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기사명 저자명 페이지 원문 목차
Genetic diversity and population structure of Korean soybean landrace [Glycine max (L.) Merr.] Gyu-taek Cho ;Jeongran Lee ;Jung-kyung Moon ;Mun-sup Yoon ;Hyung-jin Baek ;Jung-hoon Kang ;Tae-san K pp.83-90

High-throughput SNP genotyping by melting curve analysis for resistance to southern root-knot nematode and frogeye leaf spot in soybean Bo-Keun Ha ;H. Roger Boerma pp.91-100

Genetic analysis of clubroot resistance in Chinese Cabbage using single-spore isolates of plasmodiophora brassicae and development of RAPD markers linked to its resistance gene Kwang-soo Cho ;Su-young Hong ;Young-han Han ;Bong-kyeong Yoon ;Seoung-ryeol Ryu ;Jong-gyu Woo pp.101-106

A high-efficiency direct somatic embryogenesis system for strawberry (fragaria x ananassa duch.) cultivar chandler Amjad M. Husaini ;Samina Aquil ;Mukhtar Bhat ;Tabassum Qadri ;Kamaluddin ;Malik Zainul Abdin pp.107-110

Waterlogging effects on nitrogen accumulation and N₂fixation of supernodulating soybean mutants Jong-tag Youn ;Kyujung Van ;Jae-eun Lee ;Wook-han Kim ;Hong-tae Yun ;Young-up Kwon ;Youn-hwan Ryu ;S pp.111-118

Regeneration of plants from EMS-treated immature embryo cultures in soybean [Glycine max (L.) Merr.] Kyujung Van ;Hyun Ju Jang ;Young-eun Jang ;Suk-ha Lee pp.119-126

Acibenzolar-S-methyl (ASM)-induced resistance against tobamoviruses involves induction of RNA-dependent RNA polymerase (RdRp) and alternative oxidase (AOX) genes Kallahally Nagendra Madhusudhan ;Saligrama Adavigowda Deepak ;Harishchandra Sripathi Prakash ;Ganesh pp.127-134

Comparison of grain quality traits between japonica rice cultivars from Korea and Yunnan province of China Teng-qiong Yu ;Wenzhu Jiang ;Tae-ho Ham ;Sang-ho Chu ;Puji Lestari ;Jeong-heui Lee ;Myeong-ki Kim ;F pp.135-140

Recommendation of nitrogen topdressing rates at panicle initiation stage of rice using canopy reflectance Hung T. Nguyen ;Kyu-jong Lee ;Byun-woo Lee pp.141-150

Detection of main-effect QTLs, epistatic QTLs and QE interactions for grain appearance of brown rice (oryza sativa L.) Yang Qin ;Suk-man Kim ;Jae-keun Sohn pp.151-156

참고문헌 (59건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
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