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Purpose : The purpose of this study is to investigate the effects of Simvastatin, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast.

Materials & Methods : Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x 104 cells per plate. Each plates were incubated with 5% CO2 incubator at 37℃. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, 100μM of Simvastatin were added with Osteogenesis induction media, and classified as "test group". Those not added with Simvastatin were classified as "control group".

Results :

1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than 0.1μM.

2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than 0.1μM.

3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than 0.1μM.

4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group.

Conclusion : From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.

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기사명 저자명 페이지 원문 목차
골격성 Ⅱ급 부정교합의 악교정수술 전영미 pp.532-542

Ⅱ급 부정교합의 악정형 치료 유형석 ;차정열 pp.543-552

Ⅱ급 부정교합의 고정식 교정치료 역학 임성훈 pp.553-562

Simvastatin이 UMR-106 세포의 조골세포 형성에 미치는 영향 황의관 ;류동목 ;지유진 ;이덕원 ;이현우 pp.563-573

참고문헌 (32건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 MERCK & CO., INC. ZORCO (SIMVASTATIN); DISCRIPTION. 미소장
2 Therapeutic efficacy of the lipid-lowering armamentarium: the clinical benefits of aggressive lipid-lowering therapy. 네이버 미소장
3 Rogers MJ. Statins: lower lipids and better bones? Nature Medicine 2000;6:21-23. 미소장
4 Stimulation of Bone Formation In vitro and in Rodents by Statins 네이버 미소장
5 Statins augment vascular endothelial growth factor expression in osteoblastic cells via inhibition of protein prenylation. 네이버 미소장
6 Calcyclin, a Ca2+ ion-binding protein, contributes to the anabolic effects of simvastatin on bone. 네이버 미소장
7 Edwards CJ, Spector TD. Statin as modulators of bone formation. Arthritis Res 2002;1:151-153. 미소장
8 Role of the cholesterol biosynthetic pathway in osteoblastic differentiation of marrow stromal cells. 네이버 미소장
9 Lovastatin Promotes Cartilage Growth But Inhibits Endochondral Ossification of the Cranial Base in Organ Culture 네이버 미소장
10 von Stechow D, Fish S, Yahalom D et al. Does simvastatin stimulate bone formation in vivo? BMC Musculoskeletal Disorder 2003;4:8-17. 미소장
11 Use of statins and risk of fractures. 네이버 미소장
12 Effects of simvastatin on bone turnover and BMD: a 1-year randomized controlled trial in postmenopausal osteopenic women. 네이버 미소장
13 Paradoxical Effects of Statins on Aortic Valve Myofibroblasts and Osteoblasts: Implications for End-Stage Valvular Heart Disease 네이버 미소장
14 The effect of simvastatin on the proliferation and differentiation of human bone marrow stromal cells. 소장
15 Effect of statins on bone mineral density and bone histomorphometry in rodents. 네이버 미소장
16 Garrett R, Mundy GR. The role of statins as potential targets for bone formation. Arthritis Res 2002;4:237-240. 미소장
17 Statin-induced Ras activation integrates the phosphatidylinositol 3-kinase signal to Akt and MAPK for bone morphogenetic protein-2 expression in osteoblast differentiation. 네이버 미소장
18 Vasculotropin/Vascular Endothelial Growth Factor Induces Differentiation in Cultured Osteoblasts 네이버 미소장
19 Simvastatin Promotes Osteoblast Differentiation and Mineralization in MC3T3-E1 Cells 네이버 미소장
20 Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. 네이버 미소장
21 Atorvastatin inhibits hypercholesterolemia-induced cellular proliferation and bone matrix production in the rabbit aortic valve. 네이버 미소장
22 Effect of Hydroxymethylglutaryl Coenzyme A Reductase Inhibitors on the Progression of Calcific Aortic Stenosis 네이버 미소장
23 Pleiotropic Effects of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Inhibitors 네이버 미소장
24 Simvastatin did not prevent nor restore ovariectomy-induced bone loss in adult rats 네이버 미소장
25 Protein prenyltransferases. 네이버 미소장
26 Fisher JE, Halasy JM, Masarachia PJ et al. Alendronate mechanism of action: inhibition of osteoclastogenesis and bone resorption are mimicked by lovastatin and blocked by geranylgeraniol. Bone 1998;23:S339. 미소장
27 Fisher JE, Rogers MJ, Halasy JM et al. Alendronate mechanism of action: geranylgeraniol, an intermediate in the mevalonate pathway, prevents inhibition of osteoclast formation, bone resorption, and kinase activation in vitro. Cell Biology 1999;96:133-138. 미소장
28 Prevention of osteocyte and osteoblast apoptosis by bisphosphonates and calcitonin. 네이버 미소장
29 Thunyakitpisal PD, Chaisuparat R. Simvastatin, an HMG-CoA Reductase Inhibitor, reduced the Expression of Matrix Metalloproteinase-9 (Gelatinase B) in Osteoblastic Cell and HT1080 Fibrosarcoma Cells. J Pharmacol Sci 2004;94:403- 409. 미소장
30 Fromigué O, Haÿ E, Modrowski D et al. RhoA GTPase inactivation by statins induces osteosarcoma cell apoptosis by inhibiting p42/p44- MAPKs-Bcl signaling independently of BMP-2 and cell differentiation. Cell Death and Differentiation 2006;13:1845-1856. 미소장
31 Lovastatin induces apoptosis of anaplastic thyroid cancer cells via inhibition of protein geranylgeranylation and de novo protein synthesis. 네이버 미소장
32 Cafforio P, Dammacco F, Gernone A, Silvestris F. Statins activate the mitochondrial pathway of apoptosis in human lymphoblasts and myeloma cells. Carcinogenesis 2003;26:883-891. 미소장

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