Saccharomycopsis lipolytica의 생육에 미치는 Itaconic Acid의 영향 / 조석금 1
ABSTRACT 1
I. 서론 1
II. 재료 및 실험방법 2
1. 사용균주 및 기본배지 2
2. 배양과 발효조건 2
3. 분석방법 2
4. 세포의 toluene 처리 2
5. 효소활성 측정 3
III. 결과 및 고찰 3
1. Isocitrate lyase의 유도 3
2. Itaconate에 의한 isocitrate의 저해 3
3. 균의 생육에 대한 itaconate의 영향 4
4. 산 생성에 대한 itaconate의 영향 5
IV. 요약 6
〈참고문헌〉 6
초록보기
Effect of itaconic acid upon citrate and isocitrate production of Saccharomycopsis lipolytica were invesetigated. The percent inhibition of isocitrate lyase activity was about 80% at 0.8mM itaconate concentration, with Ki velue of 0.17mM in the clavage reaction. Inhibitory effect of itaconate at 20mM on Sacch. lipolytica growth was significant on n-hexadecane medium. whereas almost no inhibitory effect on glucose medium. Increasing itaconate concentration in n-hexane medium improved itaconate production up to 80% but no differance was found in glucose medium.