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Contents

Selection of tropane alkaloids high-producing lines by single cell cloning of Hyoscyamus niger L. root cultures / Ji-Yun Min ; Dong-Jin Park ; Mi-Jin Jeong ; Hyun-Jin Song ; Seung-Mi Kang ; Young-Min Kang ; Myung-Suk Choi 1

Abstract 1

Introduction 1

Materials and Methods 2

1. Plant material and callus culture 2

2. Single cell cloning and root regeneration 2

3. Selection of high producing cell lines 2

4. Stability of production of high producing root lines 2

5. Statistical analysis 2

Results and Discussion 2

1. Single cell cloning and root regeneration 2

2. Establishment of Dragendorff's reagent method 2

3. Selection of high producing root line 3

4. Contents of tropane alkaloids in selected lines 4

5. Stability of tropane alkaloids level in selected lines 5

Acknowledgements 5

Literature Cited 5

초록보기

Hyoscyamus species is sources of the hypnotic and sedative drugs hyoscyamine and scopolamine. Single cells of Hyoscyamus niger were dissociated from suspension cultures and adventitious roots obtained from single-cell clones which were cultured on B5 medium containing 3% (w/v) sucrose, 0.1 mg/L IBA and 0.4% (w/v) gelrite. H. niger adventitious root lines showed wide variation in tropane alkaloids production and growth. An effective selection of 200 root lines was made possible by the application of the ‘Dragendorff’s reagent’ for qualitative detection of the alkaloids from root. A high correlation coefficient (r=0.9390) was observed between the values obtained with the two methods based on HPLC and Dragendorff’s reagent analysis. Among the selected roots, the highest scopolamine content was 16.64 mg/g DW (Hn-59), which was 8.82-fold more productive than the lowest alkaloid producing line (Hn-25). Here, we established an efficient selection method on tropane alkaloids production and suggest that the Dragendorff’s reagent is of great practical value in selection of invisible compounds.

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참고문헌 (22건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Nutrient requirements of suspension cultures of soybean root cells 네이버 미소장
2 Grieve, M. 1977. In a modern herbal (Leyel DF, ed.),Jonathan Cape, London. pp. 397-404. 미소장
3 Intercellular and Intercultural Heterogeneity in Secondary Metabolite Accumulation in Cultures of Catharanthus roseus following Cell Line Selection 네이버 미소장
4 Tropane Alkaloid Production in Hyoscyamus Root Cultures 네이버 미소장
5 Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering 네이버 미소장
6 Optimal Culture Conditions and XAD Resin on Tropane Alkaloid Production in Scopolia parviflora Hairy root Cultures 소장
7 Maldonado-Mendoza, I.E., Ayora-Talavera, T. and Loyola- Vargas, V.M. 1992. Tropane alkaloid production in root cultures of Datura stramonium. In Vitro Cellular and Development Biology-Plant 28: 67-72. 미소장
8 Maldonado-Mendoza, I.E., Ayora-Talavera, T. and Loyola-Vargas, V.M. 1993. Establishment of hairy root cultures of Datura stramonium. Plant Cell, Tissue and Organ Culture 33: 321-329. 미소장
9 Analysis of pigmentation in individual cultured plant cells using an image processing system 네이버 미소장
10 Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus 네이버 미소장
11 Lee, O.S., Kang, Y.M., Jung, H.Y., Min, J.Y., Kang, S.M., Karigar, C.S., Prasad, D.T. and Bahk, J.D. 2005. Choi MS. Enhanced production of tropane alkaloids in Scopolia parviflora by introducing the PMT (putrescine N-methyltransferase) gene. In Vitro Cellular and Development Biology - Plant 41: 167-172. 미소장
12 Selection of high nicotine-producing cell lines of tobacco callus by single-cell cloning 네이버 미소장
13 Variation in the Tropane Alkaloid Content of Hyoscyamus muticus Plants and Cell Culture Clones. 네이버 미소장
14 Biotechnological applications of plant cells in culture 네이버 미소장
15 Shinsuke, O. and Verpoorte, R. 1993. Some accounts of variation (heterogeneity and/or instability) in secondary metabolites production by plant cell cultures. Annual rep. of natural science and home economics, Kinjo Gakuin College. 32: 9-23. 미소장
16 Tropane alkaloids in the adventitious and hairy root cultures of solanaceous plants 네이버 미소장
17 Variation of Alkaloid Production in Nicotiana rustica Callus Cultures 네이버 미소장
18 The selection of cultured plant cell lines producing high levels of biotin 네이버 미소장
19 Yamada, Y. 1984. Selection of cell lines for high yields of secondary metabolites. In: Cell Culture and Somatic Cell Genetics of Plant 1:629-636. 미소장
20 Production of tropane alkaloids in cultured cells of Hyoscyamus niger. 네이버 미소장
21 Yukimune, Y., Hara, Y. and Yamada, Y. 1992. Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection. Bioscience Biotechnology & Biochemistry 58: 1443-1446. 미소장
22 Zenk, M.H., El-Shagi, H., Arens, H., Stockigt, J., Weiler, E.W. and Deus, B. 1977. In Plant Tissue Culture and Its Bio-technological Application (Barz W, Reinhard E & Zenk MH eds.) Springer, Berlin. pp. 27. 미소장

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