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The cyclic undecapeptide cyclosporin A (CyA), one of the

most valuable immunosuppressive drugs, is produced

nonribosomally by a multifunctional cyclosporin synthetase

enzyme complex by the filamentous fungus Tolypocladium

niveum. To increase CyA productivity by wild-type T. niveum

(ATCC 34921), random mutagenesis was first performed

using an antifungal agar-plug colony assay (APCA) selection

approach. This generated a mutant strain producing

more than 9-fold greater CyA than the wild-type strain.

Additionally, a foreign bacterial gene, Vitreoscilla hemoglobin

gene (VHb), was transformed via protoplast regeneration

and its transcription was confirmed by RT-PCR in the

UV-irradiated mutant cell. This led to an additional 33.5%

increase of CyA production. Although most protoplastregenerated

T. niveum transformants tend to lose CyA

productivity, the optimized combination of random

mutagenesis and protoplast transformation described here

should be an efficient strategy to generate a commercially

valuable, yet metabolite low-producing, fungal species,

such as CyA-producing T. niveum.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Development and characterization of new microsatellite markers for the oyster mushroom (Pleurotus ostreatus) Ma, Kyung-Ho ;Gi-An Lee ;Sok-Young Lee ;Jae-Gyun Gwang ;Tae-San Kim ;Won-Sik Kong ;Kyoung-In Seo ;Ga pp.851-857

Abundance and structure of microbial loop components (Bacteria and Protists) in lakes of different trophic status Chróst, Ryszzard J ;Adamczewski Tomasz ;Krystyna Kalinowska ;Agnieszka Skowronska pp.858-868

Combination strategy to increase cyclosporin A productivity by Tolypocladium niveum using random mutagenesis and protoplast transformation Lee, Mi-Jin ;Cac Thi Phung Duong ;Kyuboem Han ;Eung-Soo Kim pp.869-872

Novel alkali-stable, cellulase-free xylanase from deep-sea Kocuria sp. Mn22 Li, Chanjuan ;Yuzhi Hong ;Zongze Shao ;Ling Lin ;Xiaoluo Huang ;Pengfu Liu ;Gaobing Wu ;Xin Meng ;Zi pp.873-880

Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2 Lee, Su-Jin ;Pil-Soo Seo ;Chul Ho Kim ;Ohsuk Kwon ;Byung-Ki Hur ;Jeong-Woo Seo pp.881-887

Lipase diversity in glacier soil based on analysis of metagenomic DNA fragments and cell culture Zhang, Yuhong ;Pengjun Shi ;Wanli Liu ;Kun Meng ;Yingguo Bai ;Guozeng Wang ;Zhichun Zhan ;Bin Yao pp.888-897

Identification of Salmonella pullorum genomic sequences using suppression subtractive hybridization Li, Qiuchun ;Yaohui Xu ;Xinan Jiao pp.898-903

In situ single cell monitoring by isocyanide-functionalized Ag and Au nanoprobe-based Raman spectroscopy Lee, So Yeong ;Soo Hwa Jang ;Myung-Haing Cho ;Young Min Kim ;Keun Chang Cho ;Pan Dong Ryu ;Myoung-Se pp.904-910

Monitoring of cleavage preference for caspase-3 using recombinant protein substrates Park, Kyoungsook ;So Yeon Yi ;Un-Lyoung Kim ;Chang-Soo Lee ;Jin Woong Chung ;Sang J. Chung ;Moonil K pp.911-917

Carotenogenesis in Haematococcus lacustris :role of protein tyrosine phosphatases Park, Jae-Kweon ;Phuong Ngoc Tran ;Jeong-Dong Kim ;Seong-Joo Hong ;Choul-Gyun Lee pp.918-921

Studies on microbial transformation of meloxicam by fungi Shyam Prasad, G ;S. Girisham ;S. M. Reddy pp.922-931

Inactivation of the wall-associated De-N-acetylase (PgdA) of Listeria monocytogenes results in greater susceptibility of the cells to induced autolysis Popowska, Magdalena ;Monika Kusio ;Paulina Szymañska ;Zdzislaw Markiewicz pp.932-945

Atromentin-induced apoptosis in human leukemia U937 cells Kim, Jin Hee ;Choong Hwan Lee pp.946-950

Study of macrophage activation and structural characteristics of purified polysaccharides from the fruiting body of Hericium erinaceus Lee, Jong Seok ;Kyoung Min Min ;Jae Youl Cho ;Eock Kee Hong pp.951-959

Seed-conjugated polymer bead for β2-microglobulin removal at neutral pH Kim, Mira ;Sungsoo Kang ;Eun-Kyung Myung ;Minkoo Ahn ;Jeong-Hyun Choi ;Seung R. Paik ;Yoon-Sik Lee pp.960-965

Optimization of the functional expression of Coprinus cinereus peroxidase in Pichia pastoris by varying the host and promoter Kim, Su-Jin ;Jeong-Ah Lee ;Yong-Hwan Kim ;Bong-Keun Song pp.966-971

Application of a fed-batch bioprocess for the heterologous production of hSCOMT in Escherichia coli Passarinha, L. A ;M. J. Bonifácio ;J. A. Queiroz pp.972-981

Evaluation of optimal culture conditions for recombinant ghost bacteria vaccine production with the antigen of Streptococcus iniae GAPDH Ra, Chae-Hun ;Yeong-Jin Kim ;So-Jin Park ;Chang-Wha Jeong ;Yoon-Kwon Nam ;Ki-Hong Kim ;Sung-Koo Kim pp.982-986

A novel tannase from the xerophilic fungus Aspergillus niger GH1 Marco, Mata-Gómez ;Luis V. Rodriguez ;Erika L. Ramos ;Jacqueline Renovato ;Mario A. Cruz-Hernández pp.987-996

Characterization of a 27 kDa fibrinolytic enzyme from Bacillus amyloliquefaciens CH51 isolated from Cheonggukjang Kim, Gyoung Min ;Ae Ran Lee ;Kang Wook Lee ;Ae-Yong Park ;Jiyeon Chun ;Jaeho Cha ;Young-Sun Song ;Je pp.997-1004

Evaluation of lysozyme to control vinification process and histamine production in rioja wines López, Isabel ;Pilar Santamaria ;Carmen Tenorio ;Patrocinio Garijo ;Ana Rosa Gutiérrez ;Rosa Lópe pp.1005-1012

Characterization of Bacillus polyfermenticus KJS-2 as a probiotic Kim, Kang Min ;Myo Jeong Kim ;Dong Hee Kim ;You Soo Park ;Jae Seon Kang pp.1013-1018

Electricity generation coupled with wastewater treatment using a microbial fuel cell composed of a modified cathode with a ceramic membrane and cellulose acetate film Seo, Ha Na ;Woo Jin Lee ;Tae Sik Hwang ;Doo Hyun Park pp.1019-1027

Bacterial dynamics of biofilm development during toluene degradation by Burkholderia vietnamiensis G4 in a gas phase membrane bioreactor Kumar, Amit ;Jo Dewulf ;Tom Van De Wiele ;Herman Van Langenhove pp.1028-1033

Overexpression, purification, and immunogenicity of recombinant porin proteins of Salmonella enterica serovar Typhi(S. Typhi) Verma, Shailendra Kumar ;Vandana Gautam ;Konduru Balakrishna ;Subodh Kumar pp.1034-1040

Etiologic and immunologic characteristics of thoroughbred horses with bacterial infectious upper respiratory disease at the Seoul Race Park Ryu, Seung-Ho ;Hye Cheong Koo ;Young Kyung Park ;Jun Man Kim ;Woo Kyung Jung ;William C. Davis ;Yong pp.1041-1050

Distribution of human papillomavirus type 58 variants in progression of cervical dysplasia in Korean women Bae, Jeong-Hoon ;Jo L.K. Cheung ;Sung-Jong Lee ;Alfred C.S. Luk ;Seo-Yun Tong ;Paul K.S. Chan ;Jong- pp.1051-1054

Molecular and epidemiological characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006 Baek, Kyungah ;Kwisung Park ;Eunhye Jung ;Eunhee Chung ;Joonsoo Park ;Hwajung Choi ;Seunghwa Baek ;Y pp.1055-1064

Investigation of Klebsiella pneumoniae isolates producing SHV-12 and SHV-11 β-lactamases in Korean hospitals Lee, Kyeong Min ;Jae Il Yoo ;Yong Sun Yoo ;Jung Sik Yoo ;Gyung Tae Chung ;Tae In Ahn ;Yeong Seon Lee pp.1065-1069

Cancer cell targeting with mouse TERT-specific group Ⅰ intron of Tetrahymena thermophila Ban, Guyee ;Min-Sun Song ;Seong-Wook Lee pp.1070-1076

참고문헌 (27건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Baltz, R. H. 1986. Mutagenesis in Streptomyces spp., pp. 184-190. In A. L. Demain and N. A. Solomon (eds.), Manual of Industrial Microbiology and Biotechnology. American Society for Microbiology, Washington, DC. 미소장
2 Biotechnology: Promethean Science or Obsession? 네이버 미소장
3 Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products. 네이버 미소장
4 TRANSPLANTATION TIMELINE MANKINDʼS 네이버 미소장
5 Genetic engineering of an industrial strain of Saccharopolyspora erythraea for stable expression of the Vitreoscilla haemoglobin gene (vhb) 네이버 미소장
6 Identification of TmcN as a pathway-specific positive regulator of tautomycetin biosynthesis in Streptomyces sp. CK4412. 네이버 미소장
7 Interspecies DNA microarray analysis identifies WblA as a pleiotropic down-regulator of antibiotic biosynthesis in Streptomyces. 네이버 미소장
8 Kobel, H. and R. Traber. 1982. Directed biosynthesis of cyclosporins. Eur. J. Appl. Microbiol. Biotechnol. 14: 237-240. 미소장
9 Biotechnology Journal 3/2008 네이버 미소장
10 Marshall, J. W. 1986. Factors for the process development of cyclosporine fermentation. Master’s thesis, The University of Western Ontario, Ontario, Canada. 미소장
11 Photodissociation of oxygenated cytochrome o(s) (Vitreoscilla) and kinetic studies of reassociation. 네이버 미소장
12 Pall, M. L. and J. P. Brunelli. 1993. A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites. Fungal Genet. Newsl. 40: 59-62. 미소장
13 Biotechnology and bioeconomy in China. 네이버 미소장
14 Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant chinese hamster ovary cells. 네이버 미소장
15 Vitreoscilla Hemoglobin 네이버 미소장
16 Rifai, M. A. 1969. A revision of the genus Trichoderma. Mycol. Papers 116: 1-56. 미소장
17 Rowlands, R. T. 1984. Industrial strain improvement: Mutagenesis and random screening procedures. Enzyme Microb. Tech. 6: 3-10. 미소장
18 Entomopathogenic species of the hyphomycete genus Tolypocladium 네이버 미소장
19 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
20 Stepkowski, S. M. 2004. Molecular targets for existing and novel immunosuppressive drugs. Expert Rev. Mol. Med. 2: 1-23. 미소장
21 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
22 Unusual structure of the oxygen-binding site in the dimeric bacterial hemoglobin from Vitreoscilla sp. 네이버 미소장
23 UV mutagenesis of Cupriavidus necator for extracellular production of (R)-3-hydroxybutyric acid. 네이버 미소장
24 Tolypocladium, a synomym of Beauveria. 네이버 미소장
25 Primary sequence of a dimeric bacterial haemoglobin from Vitreoscilla. 네이버 미소장
26 Lotfy, W. A., K. M. Ghanem, and E. R. El-Helow. 2007. Citric acid production by a novel Aspergillus niger isolate: I. Mutagenesis and cost reduction studies. Biores. Technol. 98: 3464-3469. 미소장
27 Applications of the VHb gene vgb for improved microbial fermentation processes. 네이버 미소장

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