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A biocontrol bacterium Bacillus licheniformis N1 grown

in nutrient broth showed no chitinolytic activity, while

its genome contains a gene which encodes a chitinase.

The gene for chitinase from B. licheniformis N1 was amplified

by PCR and the deduced amino acid sequence

analysis revealed that the chitinase exhibited over 95%

identity with chitinases from other B. licheniformis

strains. Escherichia coli cells carrying the recombinant

plasmid displayed chitinase activity as revealed by the

formation of a clear zone on chitin containing media,

indicating that the gene could be expressed in E. coli

cells. Chitinase gene expression in B. licheniformis N1

was not detected by RT-PCR analysis. The protein was

over-expressed in E. coli BL21 (DE3) as a glutathione Stransferase

fusion protein. The protein could also be

produced in B. subtilis 168 strain carrying the chitinase

gene of N1 strain. The crude protein extract from E. coli

BL21 carrying GST fusion protein or culture supernatant

of B. subtilis carrying the chitinase gene exhibited enzyme

activity by hydrolyzing chitin analogs, 4-methylumbelliferyl-

β-D-N,N'-diacetylchitobioside and 4-methylumbelliferyl-

β-D-N,N',N''-triacetylchitotrioside. These results

indicated that even though the chitinase gene is not expressed

in the N1 strain, the coding region is functional

and encodes an active chitinase enzyme. Furthermore,

B. subtilis 168 transformants expressing the chitinase

gene exhibited antifungal activity against Fulvia fulva

by suppressing spore germination. Our results suggest

that the proper engineering of the expression of the

indigenous chitinase gene, which will lead to its expression

in the biocontrol strain B. licheniformis N1, may

further enhance its biocontrol activity.

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기사명 저자명 페이지 원문 목차
Determination of genetic divergence based on DNA markers amongst monosporidial strains derived from fungal isolates of karnal bunt of wheat J. M. Seneviratne ;Atul K. Gupta ;Dinesh Pandey ;Indu Sharma ;Anil Kumar pp.303-316

Baseline sensitivity to mandipropamid among isolates of Phytophthora capsici causing phytophthora blight on pepper Ho Sun Jang ;Soo Min Lee ;Sun Bo Kim ;Joohyung Kim ;Susan Knight ;Kwee Doo Park ;Duncan McKenzie ;He pp.317-321

Molecular analysis of Botrytis cinerea causing ginseng grey mold resistant to carbendazim and the mixture of carbendazin plus diethofencarb Joohyung Kim ;Ji Young Min ;Young Seok Bae ;Heung Tae Kim pp.322-327

Use of serological-based assay for the detection of Pepper yellow leaf curl Indonesia virus Sri Hendrastuti Hidayat ;Dedek Haryadi ;Endang Nurhayati pp.328-332

Root colonization and ISR-mediated anthracnose disease control in cucumber by strain Enterobacter asburiae B1 S. Bharathkumar ;Jin Woo Park ;Ji Hee Han ;Kyungseok Park pp.333-343

Characterization of a chitinase gene exhibiting antifungal activity from a biocontrol bacterium Bacillus licheniformis N1 Kwang Youll Lee ;Kwang Ryool Heo ;Ki Hyuck Choi ;Hyun Gi Kong ;Jaesung Nam ;Young Byung Yi ;Seung Hw pp.344-351

Direct antimicrobial activity and induction of systemic resistance in potato plants against bacterial wilt disease by plant extracts M. A. E. Hassan ;M. F. F. Bereika ;H. I. G. Abo-Elnaga ;M. A. A. Sallam pp.352-360

Enhancing the efficacy of Burkholderia cepacia B23 with calcium chloride and chitosan to control anthracnose of papaya during storage M. A. Rahman ;T. M. M. Mahmud ;J. Kadir ;R. Abdul Rahman ;M. M. Begum pp.361-368

Antiviral effects of the culture filtrate from Serratia marcescens Gsm01, against Cucumber mosaic virus (CMV) Shree Prasad Thapa ;Hye Jin Lee ;Duck Hwan Park ;Sam-Kyu Kim ;Jun Mo Cho ;Saeyoull Cho ;Jang Hyun Hu pp.369-375

Effects of colloidal silver nanoparticles on sclerotium-forming phytopathogenic fungi Ji Seon Min ;Kyoung Su Kim ;Sang Woo Kim ;Jin Hee Jung ;Kabir Lamsal ;Seung Bin Kim ;Mooyoung Jung ; pp.376-380

Identification of a pathogen-induced Glycine max transcription factor GmWRKY1 Sang Gu Kang ;Euiho Park ;Kum Sook Do pp.381-388

Priming of defense-related genes confers root-colonizing bacilli-elicited induced systemic resistance in pepper Jung Wook Yang ;Seung Hun Yu ;Choong-Min Ryu pp.389-399

Isolation and characterization of pathogenesis-related protein 5 (PgPR5) gene from Panax ginseng Yu-Jin Kim ;Jung-Hye Lee ;Dae-Young Jung ;Gayathri Sathiyaraj ;Ju-Sun Shim ;Jun-Gyo In ;Deok-Chun Ya pp.400-407

Evaluation of bacterial blight resistance using SNP and STS marker-assisted selection in aromatic rice germplasm Jeong Soon Kim ;Jae Gyun Gwang ;Ki Hun Park ;Chang Ki Shim pp.408-416

Effects of recombination on the pathogenicity and evolution of Pepper mottle virus Miranda Gilda Jonson ;Jang-Kyun Seo ;Hong-Soo Choi ;Jeong-Soo Kim ;Kook-Hyung Kim pp.417-421

Field evaluation of mungbean recombinant inbred lines against mungbean yellow mosaic disease using new disease scale in Thailand Khalid P. Akhtar ;R. Kitsanachandee ;P. Srinives ;G. Abbas ;M. J. Asghar ;T. M. Shah ;B. M. Atta ;O. pp.422-428

Bacterial fruit rot of apricot caused by Burkholderia cepacia in China Yuan Fang ;Bin Li ;Fang Wang ;Baoping Liu ;Zhiyi Wu ;Ting Su ;Wen Qiu ;Guanlin Xie pp.429-432

참고문헌 (35건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Fungal cell wall chitinases and glucanases. 네이버 미소장
2 Cell wall chemistry, morphogenesis, and taxonomy of fungi. 네이버 미소장
3 Induced Biochemical Mutations in Bacillus subtilis 네이버 미소장
4 Biotechnology: Acquired or Consumed? 네이버 미소장
5 The molecular biology of chitin digestion 네이버 미소장
6 Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. 네이버 미소장
7 Biotechnology and bioeconomy in China. 네이버 미소장
8 Emmert, E. A. B. and Handelsman, J. 1999. Biocontrol of plant disease: a (Gram-) positive perspective. FEMS Microbiol. Lett. 171:1-9. 미소장
9 Commercialization and implementation of biocontrol. 네이버 미소장
10 Fravel, D. R., Connick Jr., W. J. and Lewis, J. A. 1998. Formulation of microorganisms to control plant diseases. In: Formulation of microbial pesticides: Beneficial microorganisms, nematodes and seed treatments, ed. by H. D. Burges, pp. 187- 202. Kluwer Academic Publishers, Dordrecht, The Netherlands. 미소장
11 Haas, D. and Défago, G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat. Rev. Microbiol. 3:307-319. 미소장
12 Biocontrol of Soilborne Plant Pathogens 네이버 미소장
13 New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. 네이버 미소장
14 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
15 Characterization of a chitinase gene from Stenotrophomonas maltophilia strain 34S1 and its involvement in biological control. 네이버 미소장
16 Lane, L. C. 1978. A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis. Anal. Chem. 86:655-664. 미소장
17 Biochemical and molecular characterization of three barley seed proteins with antifungal properties. 네이버 미소장
18 Lee, J. P., Lee, S.-W., Kim, C. S., Son, J. H., Song, J. H., Lee, K.Y., Kim, H. J., Jung, S. J. and Moon, B. J. 2006. Evaluation of formulations of Bacillus licheniformis for the biological control of tomato gray mold caused by Botrytis cinerea. Biol. Control 37:329-337. 미소장
19 Antifungal, Synergistic Interaction Between Chitinolytic Enzymes from Trichoderma harzianum and Enterobacter cloacae 네이버 미소장
20 The Role of Chitinase in the Antifungal Activity of Bacillussp. 739 네이버 미소장
21 A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037. 네이버 미소장
22 Bacillus lipopeptides: versatile weapons for plant disease biocontrol 네이버 미소장
23 Plant Pathology (4th edn) 네이버 미소장
24 Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species. 네이버 미소장
25 Plant and Bacterial Chitinases Differ in Antifungal Activity 네이버 미소장
26 Rodriguez-Kabana, R., Godoy, G., Morgan-Jones, G. and Shelby, R. A. 1983. The determination of soil chitinase activity: conditions for assay and ecological studies. Plant Soil 75:95-106. 미소장
27 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning; a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 미소장
28 Antifungal proteins. 네이버 미소장
29 Biological control of fusarium wilt of cucumber by chitinolytic bacteria. 네이버 미소장
30 Multiple Chitinase Enzymes from a Single Gene of Bacillus licheniformis TP-1 네이버 미소장
31 The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. 네이버 미소장
32 Isolation and molecular characterization of chitinase-deficient Bacillus licheniformis strains capable of deproteinization of shrimp shell waste to obtain highly viscous chitin. 네이버 미소장
33 Family 19 chitinases of Streptomyces species: characterization and distribution. 네이버 미소장
34 Microbial interactions and biocontrol in the rhizosphere 네이버 미소장
35 Xue, G.-P., Johnson, J. S. and Dalymple, B. P. 1999. High osmolarity improves the electro-transformation efficiency of the gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J. Microbiol. Methods 34:183-191. 미소장

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