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Objects: The aim of this study was to develop a new caries activity test based on colorimetric changes that could better reflect the amount of acid produced by oral bacteria as a broad spectrum of color than the previous test. The optimal candidates were combinations of pH indicators, and these were evaluated in pH buffering solutions and using dental plaque.
Materials and Methods: Six pH indicators,Bromocresol-purple (BCP), Bromocresol-green (BCG), Methyl-red (MR), Methyl-orange (MO), Resazurin (R), and Naphthyl-red (NR), were selected to show different colorsat various pH environments (range pH 3.0 ∼ 7.0). BCP and BCG are pH indicators that are already used in the Cariostat®, and they can show color changes from green to yellow. This study tried to broaden the color change spectrum from blue to red to give a more distinct differentiation at various pHs. Four pH indicators (MR, MO, R and NR) were blended with BCP and BCG. Each combination of the three indicators was assessed in different pH buffer systems: pH 7.0, 6.0, 5.0, 4.0, and 3.0. The selected combinations of pH indicators were applied to human dental plaque from 11 subjects (mean age: 27) to confirm the reproducibility of the in vitro results.
Results: According to the in vitro buffer system, the mixture of BCP, BCG and MR did not show any differences between pH 4.0 and pH 3.0. On the other hand, the mixtures of BCP-BCG-MO (ratio 2:1:1.5), BCP-BCG-R (1.5:1:0.5) and BCP-BCG-NR (2:1:1.5) showed distinguishable color changes from pH 7.0 to pH 3.0. Among the three candidates, the BCP-BCG-NR mixture showed the best color differences in the buffer solution and the human dental plaque cultivated solutions at various pHs.
Conclusion: This study evaluated a new colorimetric caries activity test that used a combination of several pH indicators. The new system can easily detect various pH environments from organic acid fermentation by using a wider range of colors (blue ? dark green ? green ? orange ? red).권호기사
참고문헌 (29건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | Acid profiles and pH of carious dentin in active and arrested lesions.  |
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| 2 | The role of sugar in the aetiology of dental caries |
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| 3 | The role of sugar in the aetiology of dental caries |
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| 4 | Dental caries and caries-associated microorganisms in Uruguayan preschool children. |
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| 5 | A new method for the estimation of mutans streptococci in human saliva. |
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| 6 | A rapid and quantitative detection system for Streptococcus mutans in saliva using monoclonal antibodies. |
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| 7 | The determination of antibody to Streptococcus mutans serotypes in saliva for children ages 3 to 7 years. |
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| 8 | Bacterial Diversity within the Human Subgingival Crevice |
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| 9 | A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: an alternative to Streptococcus mutans and the specific-plaque hypothesis. |
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| 10 | Wolff MS, Larson C. The cariogenic dental biofilm: good, bad or just something to control? Braz Oral Res 2009;23(l):31-38. | 미소장 |
| 11 | Caries ecology revisited: microbial dynamics and the caries process. |
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| 12 | Strain-related acid production by oral streptococci. |
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| 13 | Bazarragchaa T, Nishimura M, Hulan U, Matsumura S. The differences In acid-producing abilities among Streptococcus sobrinus. Pediatr Dent J 2001;11(1):37-42. | 미소장 |
| 14 | A Quantitative Method for Estimating Bacillus Acidophilus in Saliva |
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| 15 | A Simple Colorimetric Method for the Estimation of Relative Numbers of Lactobacilli in the Saliva |
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| 16 | Shimono T, Sobue S. A new colormetric caries activity test. Dent Outlook 1974;43(6):829-835. | 미소장 |
| 17 | Shimono T. 齲蝕活動性 試驗(ヵリオスタツト)の 細菌學的 なら びに 疫學的硏究. 小兒齒科學雜誌 1983;21(1):107-130. | 미소장 |
| 18 | Using a Caries Activity Test to Predict Caries Risk in Early Childhood |
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| 19 | A CLINICAL COMPARATIVE STUDY ON THE DIAGNOSTIC POWER BETWEEN SOME ORAL ENVIRONMENTAL TESTS AND ORAL STATUS OF INDIVIDUALS |
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| 20 | A CLINICAL STUDY FOR CARIES ACTIVITY OF PRESCHOOL CHILDREN USING CAR10STAT | 소장 |
| 21 | Beynon RJ, Easterby JS. Buffer solutions. The basics. Oxford: Oxford University Press;1996:1-6. | 미소장 |
| 22 | Food and drink consumption, sociodemographic factors and dental caries in 4-5-year-old children in Amman, Jordan. |
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| 23 | Bracquart P. An agar medium for the differential enumeration of Streptococcus thermophilus and Lactobacillus bulgaricus in yoghurt. J Appl Bacteriol 2008;51(2):303-305. | 미소장 |
| 24 | de MAN JC, Rogosa M, Elisabeth SM. A medium for the cultivation of lactobacilli. J Appl Bacteriol 1960;23(1):130-135. | 미소장 |
| 25 | Developments in dental plaque pH modelling. |
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| 26 | Comparison among a dip-slide test (Dentocult), plate count, and Snyder test for estimating number of lactobacilli in human saliva. |
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| 27 | A modified snyder test for caries-activity in humans |
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| 28 | Comparison of plaque samples and saliva samples using the CAT21 Test (Cariostat method) |
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| 29 | Degradation of acid orange 7 in an aerobic biofilm |
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