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Molecular function of the expansin superfamily has been highlighted for cellulosic biomass conversion. In this re-port, we identified a new bacterial expansin subfamily by analysis of related bacterial sequences and biochemically examined a member of this new subfamily from Hahella chejuensis (HcEXLX2). Among the various complex poly-saccharides tested, HcEXLX2 bound most efficiently to cellulose. The relative binding constant (Kr) against Avicel was 2.1 L g-1 at pH 6.0 and 4?C. HcEXLX2 enhanced the activity of cellulase, producing about 4.6 times more hy-drolysis product after a 36 h reaction relative to when only cellulase was used. The extension strength test on filter paper indicated that HcEXLX2 has a texture loosening effect on filter paper, which was 53% of that observed for 8 M urea treatment. These activities, compared with a cellu-lose binding domain from Clostridium thermocellum, im-plied that the synergistic effect of HcEXLX2 comes from not only binding to cellulose but also disrupting the hy-drogen bonds in cellulose. Based on these results, we suggest that the new bacterial expansin subfamily func-tions by binding to cell wall polysaccharides and increas-ing the accessibility of cell wall degrading enzymes.

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Regulation of actin cytoskeleton dynamics in cells Sung Haeng Lee ;Roberto Dominguez pp.311-325

Physiological consequences of programmed necrosis, an alternative form of cell demise Young Sik Cho ;Seung Yeon Park ;Hee Suk Shin ;Francis Ka-Ming Chan pp.327-332

Abelson virus transformation prevents TRAIL expression by inhibiting FoxO3a and NF-κB Mary K. Wilson ;Sarah M. McWhirter ;Rupesh H. Amin ;Dan Huang ;Mark S. Schlissel pp.333-341

Lack of association of MTHFR gene polymorphisms with the risk of osteonecrosis of the femoral head in a Korean population Tae-Ho Kim ;Jung Min Hong ;Hyun-Ju Kim ;Eui Kyun Park ;Shin-Yoon Kim pp.343-348

Amelioration of streptozotocin-induced diabetes by Agrocybe chaxingu polysaccharide Byung Ryong Lee ;Yeom Pyo Lee ;Dae Won Kim ;Ha Yong Song ;Ki-Yeon Yoo ;Moo Ho Won ;Tae-Cheon Kang ;K pp.349-354

Identification and characterization of CMTM4, a novel gene with inhibitory effects on HeLa cell growth through inducing G2/M phase accumulation Markus Plate ;Ting Li ;Yu Wang ;Xiaoning Mo ;Yingmei Zhang ;Dalong Ma ;Wenling Han pp.355-361

(An)Archaeal NADH oxidase causes damage to both proteins and nucleic acids under oxidative stress Baolei Jia ;Sangmin Lee ;Bang P. Pham ;Yoon Seung Cho ;Jae-Kyung Yang ;Hee-Seop Byeon ;Jong Cheol Ki pp.363-371

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(An)Expansin-like protein from Hahella chejuensis binds cellulose and enhances cellulase activity Hee Jin Lee ;Saeyoung Lee ;Hyeok-jin Ko ;Kyoung Heon Kim ;In-Geol Choi pp.379-385

IP₃-induced cytosolic and nuclear Ca^2+^ signal in HL-1 atrial myocytes :possible role of IP₃ receptor subtypes Joon-Chul Kim ;Min-Jeong Son ;Krishna P. Subedi ;Do Han Kim ;Sun-Hee Woo pp.387-395

Structural basis for the reaction mechanism of UDP-glucose pyrophosphorylase Hun Kim ;Jongkeun Choi ;Truc Kim ;Neratur K. Lokanath ;Sung Chul Ha ;Se Won Suh ;Hye-Yeon Hwang ;Kye pp.397-405

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Tyrosylprotein sulfotransferase regulates collagen secretion in Caenorhabditis elegans Tai Hoon Kim ;Do Hyun Kim ;Hyung Wook Nam ;Sang Yoon Park ;Jaegal Shim ;Jin Won Cho pp.413-418

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TGF-β3 inhibits chondrogenesis by suppressing precartilage condensation through stimulation of N-cadherin shedding and reduction of cRREB-1 expression Eun-Jung Jin ;Kwang Sook Park ;Dongkyun Kim ;Young-Sup Lee ;Jong Kyung Sonn ;Jae Chang Jung ;Ok-Sun pp.425-432

참고문헌 (30건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Adney, B., and Baker, J. (1996). Chemical Analysis and Testing Task: LAP-006 (Measurement of cellulase activities) (Golden, USA: National Renewable Energy Laboratory). 미소장
2 Cellulose-Binding Domains Promote Hydrolysis of Different Sites on Crystalline Cellulose 네이버 미소장
3 The growing world of expansins. 네이버 미소장
4 Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase 네이버 미소장
5 The adsorption of a bacterial cellulase and its two isolated domains to crystalline cellulose. 네이버 미소장
6 meta-MEME: Motif-based hidden Markov models of protein families 네이버 미소장
7 Synergism of Cellulases from Trichoderma reesei in the Degradation of Cellulose 네이버 미소장
8 Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. 네이버 미소장
9 Kende, H., Bradford, K.J., Brummell, D.A., Cho, H.T., Cosgrove, D.J., Fleming, A.J., Gehring, C., Lee, Y., McQueen-Mason, S., Rose, J.K.C., et al. (2004). Nomenclature for members of the expansin superfamily of genes and proteins. Plant Mol. Biol. 55, 311-314. 미소장
10 Crystal Structure and Activity of Bacillus subtilis YoaJ (EXLX1), a Bacterial Expansin That Promotes Root Colonization 네이버 미소장
11 Biotechnology Journal 3/2008 네이버 미소장
12 Biotechnology in Turkey: an overview. 네이버 미소장
13 Biotechnology Journal 3/2008 네이버 미소장
14 Lee, J., and Kim, S.H. (2009). High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences. Protein Expr. Purif. 63, 58-61. 미소장
15 Biotechnology Journal 3/2008 네이버 미소장
16 Disruption of Hydrogen Bonding Between Plant Cell Wall Polymers by Proteins that Induce Wall Extension 네이버 미소장
17 Expansin Mode of Action on Cell Walls: Analysis of Wall Hydrolysis, Stress Relaxation, and Binding 네이버 미소장
18 Biotechnological studies in the Far-Eastern Region of Russia. 네이버 미소장
19 Expression of the flagellin gene in Borrelia is controlled by an alternative factor 네이버 미소장
20 T-Coffee: A novel method for fast and accurate multiple sequence alignment. 네이버 미소장
21 Plant degradation: a nematode expansin acting on plants. 네이버 미소장
22 Retief, J.D. (2000). Phylogenetic analysis using PHYLIP. Methods Mol. Biol. 132, 243-258. 미소장
23 Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials. 네이버 미소장
24 The expansin superfamily 네이버 미소장
25 Shin, J.-H., Jeong, D.-H., Park, M.C., and An, G. (2005). Characterization and transcriptional expression of the α-expansin gene family in rice. Mol. Cells 20, 210-218. 미소장
26 Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis. 네이버 미소장
27 Tormo, J., Lamed, R., Chirino, A.J., Morag, E., Bayer, E.A., Shoham, Y., and Steitz, T.A. (1996). Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 15, 5739-5751. 미소장
28 SUPERFAMILY--sophisticated comparative genomics, data mining, visualization and phylogeny. 네이버 미소장
29 Microplate‐based filter paper assay to measure total cellulase activity 네이버 미소장
30 Crystal structure and activities of EXPB1 (Zea m 1), a beta-expansin and group-1 pollen allergen from maize. 네이버 미소장

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