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Stimulatory effect of Cinnamomum cassia and cinnamic acid on angiogenesis through up-regulation of VEGF and Flk-1/KDR expression / Do-Young Choi ; Yong-Hyeon Baek ; Jeong-Eun Huh ; Jeong-Min Ko ; HyunSu Woo ; Jae-Dong Lee ; Dong-Suk Park 1

ABSTRACT 1

1. Introduction 1

2. Materials and methods 2

2.1. Reagents 2

2.2. Preparation of C. cassia and standardization 2

2.3. Mouse Matrigel angiogenic assay 2

2.4. Cell culture 4

2.5. Proliferation assay 4

2.6. Chemotaxis migration assay 4

2.7. Tubule-like structure assay 4

2.8. Measurement of VEGF 4

2.9. Reverse transcription polymerase chain reaction (RT-PCR) 5

2.10. Quantitative real-time PCR 5

2.11. Statistical analysis 6

3. Results 6

3.1. CCE and CA induces angiogenesis in the in vivo Matrigel assay 6

3.2. CCE and CA increases endothelial cells proliferation 7

3.3. CCE and CA increases endothelial cell migration 7

3.4. CCE and CA induces endothelial cell differentiation 7

3.5. Differential expression of VEGF isoforms, Flk-1 / KDR and Flt-1 mRNA in endothelial cells 8

3.6. CCE and CA increases VEGF, Flk-1 / KDR RNA but not Flt-1 expression in endothelial cells 8

4. Discussion 8

References 8

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