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초록보기

New multiple-stress related gene isolated from sweet potato

and designated it as MusI (multiple stress responsible gene

I). Sequence analysis revealed that its full length cDNA was

998 bp long and included a 717 bp open reading frame encoding

for 238 amino acids. Comparison of its cDNA and genomic

DNA sequence showed that 3 exons were divided by

2 introns in its ORF region. Its deduced amino acid sequence

contained a conserved rubber elongation factor (REF) domain

and showed high homology with many stress-related proteins.

Therefore, it was named MuSI (multiple stress responsible

gene I). Southern hybridization analysis indicated that the

MuSI gene may belong to a multi-gene family. Expression pattern

of the MuSI gene showed that it was differently expressed

among roots, stems, leaves, and flowers of a sweet potato,

and its expression level was especially high in flowers and

white fibrous roots. Its expression was also highly induced

by various stress signals including dehydration, high salt,

heavy metal, oxidation, and plant hormones. Stress tolerance

experiment using transgenic plants overexpressing the MuSI

gene showed that all independent transgenic tobacco lines have

enhanced tolerance to high temperature stress. Among them,

transgenic line 6 particularly showed tolerance to salt, heavy

metal, and osmotic stress as well. These results suggest that

the MuSI gene functions as a positive regulator of various

stress responses and may be useful in improving stress tolerance

of transgenic plants.

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권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
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참고문헌 (30건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
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