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New multiple-stress related gene isolated from sweet potato
and designated it as MusI (multiple stress responsible gene
I). Sequence analysis revealed that its full length cDNA was
998 bp long and included a 717 bp open reading frame encoding
for 238 amino acids. Comparison of its cDNA and genomic
DNA sequence showed that 3 exons were divided by
2 introns in its ORF region. Its deduced amino acid sequence
contained a conserved rubber elongation factor (REF) domain
and showed high homology with many stress-related proteins.
Therefore, it was named MuSI (multiple stress responsible
gene I). Southern hybridization analysis indicated that the
MuSI gene may belong to a multi-gene family. Expression pattern
of the MuSI gene showed that it was differently expressed
among roots, stems, leaves, and flowers of a sweet potato,
and its expression level was especially high in flowers and
white fibrous roots. Its expression was also highly induced
by various stress signals including dehydration, high salt,
heavy metal, oxidation, and plant hormones. Stress tolerance
experiment using transgenic plants overexpressing the MuSI
gene showed that all independent transgenic tobacco lines have
enhanced tolerance to high temperature stress. Among them,
transgenic line 6 particularly showed tolerance to salt, heavy
metal, and osmotic stress as well. These results suggest that
the MuSI gene functions as a positive regulator of various
stress responses and may be useful in improving stress tolerance
of transgenic plants.| 기사명 | 저자명 | 페이지 | 원문 | 목차 |
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| QTL detection and MAS selection efficiency for lipid content in brown rice(Oryza sativa L.) | Yang Qin ,Suk-Man Kim ,Xinhua Zhao ,Hyun-Suk Lee ,Baoyan Jia ,Kyung-Min Kim ,Moo-Young Eun ,Jae-Keun Sohn | pp.506-512 |
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| Molecular characterization and comparative analysis of two waxy alleles in barley | Jian Ma ,Qian-Tao Jiang ,Yu-Ming Wei ,Laroche Andre ,Zhen-Xiang Lu ,Guo-Yue Chen ,Ya-Xi Liu ,You-Liang Zheng | pp.513-520 |
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| (A)possible role of STRA8 as a transcriptional factor | Young-Joon Choi ,Jea-Wan Yoon ,Chul-Woong Pyo ,Jung-Ah Kim ,Seong-Ho Bae ,Sung-Soo Park | pp.521-526 |
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| Detection of transgenes in three genetically modified rice lines by fluorescence in situ hybridization | Hye Mi Park ,Eun Jin Jeon ,Nomar Espinosa Waminal ,Kong Sik Shin ,Soon Jong Kweon ,Beom-Seok Park ,Seok Cheol Suh ,Hyun Hee Kim | pp.527-531 |
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| Microsatellite analysis of the silkworm strains(Bombyx mori) :high variability and potential markers for strain identification | Kee Young Kim ,Pil Don Kang ,Kwang Gil Lee ,Hyung Keun Oh ,Min Jee Kim ,Ki-Hwan Kim ,Sang Won Park ,Sang Jae Lee ,Byung Rae Jin ,Iksoo Kim | pp.532-543 |
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| Cloning and characterization of the new multiple stress responsible gene Ⅰ(MuSⅠ) from sweet potato | Sang-Gyu Seo ,Ji-Seoung Kim ,Yu-Sun Yang ,Byung-Ki Jun ,Seung-Won Kang ,Gung-Pyo Lee ,Wook Kim ,Jong-Bo Kim ,Hyeong-Un Lee ,Sun-Hyung Kim | pp.544-552 |
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| Expressed sequence tag analysis generated from a normalized full-length cDNA library of the root-knot nematode (Meloidogyne incognita) | Man-Jung Kang ,Yong-Hwan Kim ,Bum-Soo Hahn | pp.553-562 |
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| Determining the fate of Shh-expressing cells in the diencephalon using a BAC transgenic reporter | Bumwhee Lee ,Minho Park ,Kwanghee Baek ,Jaeseung Yoon ,Yongsu Jeong | pp.563-569 |
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| Intron loss mediated structural dynamics and functional differentiation of the polygalacturonase gene family in land plants | Kyong-Cheul Park ,Soon-Jae Kwon ,Nam-Soo Kim | pp.570-577 |
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| Molecular cloning of GnRH1 gene and GTH cDNAs of the protogynous longtooth grouper, Epinephelus bruneus | Kyeong-Man Hyeon ,Hyung-Bok Jeong ,Bong-Soo Lim ,Sung-Pyo Hur ,Young-Don Lee ,Ji-Gweon Park ,Se-Jae Kim | pp.583-591 |
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| Apoptotic effect of naphthoquinone derivatives on HCT116 colon cancer cells | Young-Sam Im ,Yongseog Chung ,Dae Yeon Won ,Soo Han Kwon ,Hye-Ryun Kim ,Dong Geun Lee ,Seung-Ryul Kim ,Kyung Do Park ,Hak-Kyo Lee ,Joong-Kook Choi | pp.592-598 |
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| Stimulation of the endosomal TLR pathway enhances autophagy-induced cell death in radiotherapy of breast cancer | Su-Jin Kang ,Ji-Hye Tak ,Jung-Hyun Cho ,Hyo-Ji Lee ,Yu-Jin Jung | pp.599-606 |
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