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In 1993, after 10 years of absence, malaria re-emerged in the Republic of Korea in Gyeonggi and Gangwon Provinces near the Demilitarized Zone (DMZ). Anopheline mosquitoes are known to play a critical role in malaria transmission. Several studies have used gene expression data to examine the transcription responses of Anopheles gambiae after microbial and Plasmodium infections; however, as of now, there have been no reports of studies concerning the innate immune response and apoptosis in Anopheles sinensis. We have constructed two independent cDNA libraries from An. sinensis challenged with actinomycin-D (T, treated with apoptosis-inducing chemical) and without actinomycin-D (C, control). Analysis of the 5704 (C, 2848; T, 2856) expression sequence tags (ESTs) generated from these libraries identified 884 contigs (C, 421; T, 463) and 2180 singletons (C, 1038; T, 1142) with average lengths of 831 and 817 bp, respectively. Furthermore, comparison of our EST sequences against the eukaryotic orthologous group database showed that 4324 (75.82%) of 5704 sequences could be grouped into three major functional categories. These EST data provide the basis for further studies characterizing the molecular mechanisms of midgut apoptosis.

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기사명 저자명 페이지 원문 목차
Larval survival of two species of forensically important blowflies(Diptera: Calliphoridae) after submergence in water Devinder Singh ,Madhu Bala pp.39-45

Performance of polyvoltine silkworm bombyx mori L. on different mulberry plant varieties Md Habibullah Bahar ,Mahmud Al Parvez ,Saidur Rahman ,Rejaul Islam pp.46-52

Bioinformatic analysis and annotation of expressed sequence tags(ESTs) generated from Anopheles sinensis mosquitoes challenged with apoptosis-inducing chemical, actinomycin-D Yong Hun Jo ,Yong Seok Lee ,Se Won Kang ,Weon-Gyu Kho ,Hong Seog Park ,Sang Haeng Choi ,Yu Jung Kim ,Young Seok Hong ,Mi Young Noh ,Seung Han Oh ,Iksoo Kim ,Yeon Soo Han pp.53-59

Field efficacy and non-target effects of temephos granules against culex pipiens(Diptera: Culicidae) and microorganisms in septic tanks, Republic of Korea Joo-Oh Kang ,Dong Kwan Jeong ,Dong-Kyu Lee ,Hyesook Kang ,Martha Kiarie-Makara pp.60-65

New records of isonychia formosana, prosopistoma annamense and prosopistoma sinense(ephemeroptera) from Thailand Nisarat Tungpairojwong ,Boonsatien Boonsoong pp.66-69

First record of two cryptine genera calaminus and eurycryptus(Hymenoptera: Ichneumonidae) from Korea Seung-Ho Oh ,Ki-Beom Kim ,Seung-Lak An ,Jong-Wook Lee pp.70-73

참고문헌 (18건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403–410. 미소장
2 Dana AN, Hillenmeyer ME, Lobo NF, Kern MK, Romans PA, Collins FH (2006) Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection. BMC Genomics 7: 119. 미소장
3 Dimopoulos G, Christophides GK, Meister S, Schultz J, White KP, Barillas-Mury C et al. (2002) ion. ica 99: 8814–8819. 미소장
4 Ewing B, Green P (1998) Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Research 8: 186–194. 미소장
5 Ewing B, Hillier L, Wendl M, Green P (1998) Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Research 8: 175–185. 미소장
6 Gupta L, Kumar S, Han Y, Pimenta P, Barillas-Mury C (2005) Midgut epithelial responses of different mosquito-Plasmodium combinations: the actin cone zipper repair mechanism in Aedes aegypti. Proceedings of the National Academy of Sciences of the United States of America 102: 4010–4015. 미소장
7 Han Y, Barillas-Mury C (2002) Implications of Time Bomb model of ookinete invasion of midgut cells. Insect Biochemistry and Molecular Biology 32: 1311–1316. 미소장
8 Han Y, Thompson J, Kafatos F, Barillas-Mury C (2000) Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes. The EMBO Journal 19: 6030–6040. 미소장
9 The Role of Reactive Oxygen Species on Plasmodium Melanotic Encapsulation in Anopheles gambiae 네이버 미소장
10 Kumar S, Gupta L, Han Y, Barillas-Mury C (2004) Inducible peroxidases mediate nitration of anopheles midgut cells undergoing apoptosis in response to Plasmodium invasion. The Journal of Biological Chemistry 279: 53 475–53 482. 미소장
11 Lee W, Klein T, Kim H, Choi Y, Yoon S, Chang K et al. (2007) Anopheles kleini, Anopheles pullus, and Anopheles sinensis: potential vectors of Plasmodium vivax in the Republic of Korea. Journal of Medical Entomology 44: 1086–1090. 미소장
12 Luckhart S, Vodovotz Y, Cui L, Rosenberg R (1998) The mosquito Anopheles stephensi limits malariparasite development with inducible synthesis of nitric oxide. Proceedings National Academy of Sciences of the United States of America 95: 5700–5705. 미소장
13 Marinotti O, Nguyen Q, Calvo E, James A, Ribeiro J (2005) Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae. Insect Molecular Biology 14: 365–373. 미소장
14 Generations of sequencing technologies 네이버 미소장
15 TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets. 네이버 미소장
16 Ribeiro J (2003) A catalogue of Anopheles gambiae transcripts significantly more or less expressed following a blood meal. Insect Biochemistry and Molecular Biology 33: 865–882. 미소장
17 West African Literatures: ways of reading (review) 네이버 미소장
18 The COG database: an updated version includes eukaryotes. 네이버 미소장

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