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hFSH is a glycoprotein secreted from anterior pituitary and consists of α and β subunits. Because of its major biological functions including sperm formation in the male and for follicular growth, FSH is used to cure woman's sterility. In this study we tried to produce recombinant hFSH in vitro using a retrovirus expression vector. Two major components of the vector we constructed are: (ⅰ) a DNA fragment containing α and β genes fused by a DNA sequence coding carboxyl terminal peptide (CTP) of human chorionic gonadotropin, (ⅱ) a DNA fragment corresponding woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). Evaluation of expression profile of the recombinant FSH using reverse transcription PCR and enzyme-linked immunosorbent assay (ELISA). Among three cell lines tested, HeLa cells were the best for hFSH expression (5,395 mIU/ml), then followed by chicken embryonic fibroblast (CEF) cells and Chinese hamster ovary (CHO) cells in the order of hFSH production. In addition to the amount, the FSH produced from HeLa cells was highest in terms of biological activity which was determined by measuring cAMP.

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다중 역전사 중합효소 연쇄 반응(Multiplex RT-PCR)을 이용한 인간배아 줄기세포 및 유도만능 줄기세포의 효과적인 분화 양상 조사 김정모, 조윤정, 손온주, 홍기성, 정형민 pp.1-8

BMP4 처리에 의한 인간 배아줄기세포 유래 KDR 양성 중배엽성 세포군의 분화 양상 조사 김정모, 손온주, 조윤정, 이재호, 정형민 pp.9-15

Optimization of electrofusion condition for the production of Korean cattle somatic cell nuclear transfer embryos Sewoong Kim ,Daehwan Kim ,Yeon-Gil Jung ,Sangho Roh pp.17-22

Characterizations of cell lineage markers in the bone marrow cells of recloned GFP pigs for possible use of stem cell population Kwang-Wok Park, Sung Sik Choi, Dong Ho Lee, Hwang Lee, Seung Kyu Choi, Chang Sik Park, Sang Ho Lee pp.23-31

Hsp90 inhibitor induces cell cycle arrest and apoptosis of early embryos and primary cells in pigs Myeong-Ju Son ,Jin-Mo Park ,Sung-Hun Min ,Joo-Hee Hong ,Humdai Park ,Deog-Bon Koo pp.33-45

Estrogenic Xenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향 오신애, 박유진, 송원희, El-Sayed A. Mohamed, 방명걸 pp.47-54

고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향 하아나 ,윤진호 ,김유곤 ,조아라 ,이경림 ,공일근 pp.55-60

다양한 Percoll 세척 방법이 동결-융해된 한우 정자의 회수율 및 운동역학에 미치는 영향 윤성재 ,박유진 ,정진용 ,정규현 ,김민섭 ,유새미 ,김연희 ,권우성 ,El-Sayed A. Mohamed ,방명걸 pp.61-65

Methylation status of H19 gene in embryos produced by nuclear transfer of spermatogonial stem cells in pig Hyun Seung Lee, Sung Ho Lee, Mukesh Kumar Gupta, Sang Jun Uhm, Hoon Taek Lee pp.67-75

초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산 김은영, 박민지, 김재연, 박효영, 노은지, 노은형, 송동환, 오창언, 김영훈, 문성호, 이동선, 고문석, 류기중, 박세필 pp.77-83

Percoll 분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향 이상희 ,유한준 ,이용승 ,정희태 ,양부근 ,김대영 ,박춘근 pp.85-91

돼지 체세포복제 배반포에서 Pre-1 영역의 Non-CpG 메틸화 양상 고응규 ,임기순 ,이휘철 ,조상래 ,최선호 ,최창용 ,이풍연 ,조창연 ,조재현 ,유용희 pp.93-97

돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(TLE-1) mRNA의 발현 장예진 ,김동우 ,이용승 ,정희태 ,양부근 ,박춘근 pp.99-103

Nuclear modeling and developmental potential of bovine somatic nuclear transfer embryos cloned by two different activation methods Byeong-Gyun Jeon ,Gyu-Jin Rho pp.105-113

In Vitro에서 Retrovirus Vector System을 이용한 재조합 hFSH 유전자의 발현 민경헌, 권모선, 김태완, 구본철 pp.115-121

Establishment of porcine embryonic stem cells by aggregation of parthenogenetic embryos Jin Yu Zhang ,Yun Fei Diao ,Reza K. Oqani ,Je Sok Wo ,Dong Il Jin pp.123-129

참고문헌 (36건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Baenziger JU, Green ED (1988): Pituitary glycoprotein hormone oligosaccharides: Structure, synthesis and function of the asparagine-linked oligosaccharides on lutropin, follitropin and thyrotropin. Biochim Biophys Acta 947:287-306. 미소장
2 Bagatti GC, Datola CA, Gostoli G, Mascia M, Polletta P, Roncalli G, Simoni P (2001): Characterization and comparison of recombinant human follicle-stimulating hormones. J Clin Res 4:91-104. 미소장
3 Bergh C, Howles CM, Borg K, Hamberger L, Josefsson B, Nilsson L, Wikland M (1997): Recombinant human follicle stimulating hormone (r-hFSH; Gonal- F) versus highly purified urinary FSH (Metrodin HP): results of a randomized comparative study in women undergoing assisted reproductive techniques. Hum Reprod 12:2133-2139. 미소장
4 High throughput protein production for functional proteomics 네이버 미소장
5 Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK (1993): Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and effcient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90:8033-8037. 미소장
6 Improvement of glycosylation in insect cells with mammalian glycosyltransferases 네이버 미소장
7 Chung JY, Ahn HK, Lim SW, Sung YH, Koh YW, Park SK, Lee GM (2003): Development of recombinant Chinese hamster ovary cell lines producing human thrombopoietin or its analog. J Microbiol Biotechnol 13: 759-766. 미소장
8 Design of a Long-Acting Follitropin Agonist by Fusing the C-Terminal Sequence of the Chorionic Gonadotropin β Subunit to the Follitropin β Subunit 네이버 미소장
9 In Vitro Fertilisation 네이버 미소장
10 The program for in vitro fertilization at Norfolk. 네이버 미소장
11 Kay MA, Glorioso JC, Naldini L (2001): Viral vectors for gene therapy: the art of turning infectious agents into vehicles of therapeutics. Nature Med 7: 33-40. 미소장
12 Human Erythropoietin Induces Lung Failure and Erythrocytosis in Transgenic Mice 소장
13 Kim NS, Chang KH, Chung BS, Kim SH, Kim JH, Lee GM (2003): Characterization of humanized antibody produced by apoptosis-resistant CHO cells under sodium butyrate-induced condition. J Microbiol Biotechnol 13:926-936. 미소장
14 Kim T (2002): Retrovirus-mediated gene transfer. In Transgenic Animal Technology. Edited by Pinkert CA. 2nd ed. Academic Press, San Diego, CA, pp 173- 193. 미소장
15 The therapeutic potential of erythropoietin receptor transgenes. 네이버 미소장
16 Development and characterization of a long‐acting recombinant hFSH agonist 네이버 미소장
17 Enhanced stimulation of follicle maturation and ovulatory potential by long acting follicle-stimulating hormone agonists with extended carboxyl-terminal peptides. 네이버 미소장
18 Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles 네이버 미소장
19 Liu ML, Winther BL, Kay MA (1996): Pseudotransduction of hepatocytes by using concentrated pseudotyped vesicular stomatitis virus G glycoprotein (VSV- G)-Moloney murine leukemia virus-derived retrovirus vectors: comparison of VSV-G and amphotropic vectors for hepatic gene transfer. J Virol 70:2497- 2502. 미소장
20 Loumaye E, Campbell R, Salat-Baroux J (1995): Human follicle-stimulating hormone produced by recombinant DNA technology: a review for clinicians. Hum Reprod Update 1:188-199. 미소장
21 Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. 네이버 미소장
22 Moyle WR, Myers RV, Wang Y, Han Y, Lin W, Kelley GL, Ehrlich PH, Rao SN, Bernard MP (1998): Functional homodimeric glycoprotein hormones: implications for hormone action and evolution. Chem Biol 5:241-254. 미소장
23 Nishikawa M, Huang L (2001): Nonviral vectors in the new millennium: delivery barriers in gene transfer. Hum Gene Ther 12:861-870. 미소장
24 Out HJ, Mannaerts BMJL, Driessen SGAJ, Bennink HJTC (1996): Recombinant follicle stimulating hormone (rFSH; Puregon) in assisted reproduction: more oocytes, more pregnancies. Results from five comparative studies. Hum Reprod Update 2:162-71. 미소장
25 Out HJ., Bennink HJ, de Laat WN (1999): What are the clinical benefits of recombinant gonadotrphins? the development of recombinant FSH (Puregon): a scientific business. Hum Reprod 14:2189-2190. 미소장
26 Padmanbhan V, Chappel SC, Beitins IZ (1987): An improved in vitro bioassay for follicle-stimulating hormone (FSH): suitable for measurement of FSH in unextracted human serum. Endocrinol 121:1089- 1098. 미소장
27 Palomares LA, Estrada MS, Ramirez OT (2004): Production of recombinant protein: Challenges and solutions. Methods Mol Biol 267:15-52. 미소장
28 Pavlou AK, Reichert JM (2004): Recombinant protein therapeutics-success rates, market trends and values to 2010. Nature Biotechnol 22:1513-1519. 미소장
29 Recombinant Follicle-Stimulating Hormone: New Biotechnology for Infertility 네이버 미소장
30 Reichert Jr, LE, Dattatreyamurty B (1989): The follicle-stimulating hormone (FSH) receptor in testis: interaction with FSH, mechanism of signal transduction, and properties of the purified receptor. Biol Reprod 40:13-26. 미소장
31 Failure of combined follicle-stimulating hormone-testosterone administration to initiate and/or maintain spermatogenesis in men with hypogonadotropic hypogonadism [published erratum appears in J Clin Endocrinol Metab 1994 Apr;78(4):846] 네이버 미소장
32 Speroff L, Glass RH, Kase NG (1989): Clinical Gynecologic Endocrinology and Infertility, pp 583-609. 3rd Ed. Williams & Wikins, Baltimore, USA. 미소장
33 Expression of Biologically Active Fusion Genes Encoding the Common Subunit and the Follicle-stimulating Hormone Subunit 네이버 미소장
34 Walsh G (2006): Biopharmaceutical benchmarks. Net Biotechonol 24:769-776. 미소장
35 Production of recombinant protein therapeutics in cultivated mammalian cells. 네이버 미소장
36 Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. 네이버 미소장

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