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Endometrium undergoing hormonal change plays important roles in preparation for implantation, fetal growth, and well-being. During pregnancy, cellular remodeling and hormonal changes in endometrium could change two-pore domain K^+ channel (K_2P) expression. This study was performed to identify whether K_2P channel expression is changed in endometrium of pregnant Korean cattle, and whether the expression level is modulated by progesterone treatment. We investigated changes in the mRNA and protein expressions of K_2P channel in pregnant endometrium using RT-PCR and Western blot analyses. The expression levels of all K_2P channel mRNAs tested in this study, except that of TREK-1, were changed in the pregnant endometrium. mRNA levels of TASK-3 and TRAAK were significantly down-regulated, whereas those of TREK-2 and TRESK were up-regulated in the pregnant endometrium. In parallel with the RT-PCR results, Western blot analysis revealed up-regulations of TREK-2 (7.9-fold) and TRESK (2-fold) proteins levels in the pregnant endometrium. In addition, TREK-2 and TRESK protein levels were up-regulated in bovine endometrial cells by progesterone treatment (10 μg/ml). From these results, we suggest that the up-regulation of TREK-2 and TRESK by progesterone may contribute to the regulation of physiological changes during pregnancy.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
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참고문헌 (24건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Expression of TASK and TREK, two-pore domain K+ channels, in human myometrium. 네이버 미소장
2 Bai X, Greenwood SL, Glazier JD, Baker PN, Sibley CP, Taggart MJ and Fyfe GK. 2005b. Localization of TASK and TREK, two-pore domain K+ channels, in human cytotrophoblast cells. J. Soc. Gynecol. Investig. 12:77-83. 미소장
3 Bai X, Lacey HA, Greenwood SL, Baker PN, Turner MA, Sibley CP and Fyfe GK. 2006. TASK channel expression in human placenta and cytotrophoblast cells. J. Soc. Gynecol. Investig. 13:30-39. 미소장
4 Besana A, Robinson RB and Feinmark SJ. 2005. Lipids and two-pore domain K+ channels in excitable cells. Prostaglandins Other Lipid Mediat. 77:103-110. 미소장
5 Borna S and Sahabi N. 2008. Progesterone for maintenance tocolytic therapy after threatened preterm labour: A randomised controlled trial. Aust. N. Z. J. Obstet. Gynaecol. 48: 58-63 미소장
6 Enyeart JJ, Xu L, Danthi S, and Enyeart JA. 2002. An ACTHand ATP-regulated background K+ channel in adrenocortical cells is TREK-1. J. Biol. Chem. 277:49186-49199. 미소장
7 Gellersen B, Fernandes, MS and Brosens JJ. 2009. Non-genomic progesterone actions in female reproduction. Hum. Reprod. Update 15:119-138 미소장
8 Expression and localization of two-pore domain K(+) channels in bovine germ cells. 네이버 미소장
9 Mechanism of inhibition of TREK-2 (K2P10.1) by the Gq-coupled M3 muscarinic receptor. 네이버 미소장
10 Kelley BG and Mermelstein PG. 2011. Progesterone blocks multiple routes of ion flux. Mol. Cell. Neurosci. 48:137-141. 미소장
11 Kim D. 2003. Fatty acid-sensitive two-pore domain K+ channels. Trends Pharmacol. Sci. 24:648-654. 미소장
12 Kim D. 2005. Physiology and pharmacology of two-pore domain potassium channels. Curr. Pharm. Des. 11:2717-2736. 미소장
13 Baicalein and wogonin are activators of rat TREK‐2 two‐pore domain K+ channel 네이버 미소장
14 Kunzelmann, K. 2005. Ion channels and cancer. J. Membr. Biol. 205:159-173. 미소장
15 Cytokines in Implantation 네이버 미소장
16 Sanders KM and Koh SD. 2006. Two-pore-domain potassium channels in smooth muscles:new components of myogenic regulation. J. Physiol. 570:37-43. 미소장
17 Sano Y, Inamura K, Miyake A, Mochizuki S, Kitada C, Yokoi H, Nozawa K, Okada H, Matsushime H and Furuichi K. 2003. A novel two-pore domain K+channel, TRESK, is localized in the spinal cord. J. Biol. Chem. 278:27406-27412. 미소장
18 Shen Z, Yang Q and You Q. 2009. Researches toward potassium channels on tumor progressions. Curr. Top. Med. Chem. 9: 322-329. 미소장
19 The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm 네이버 미소장
20 Two-Pore-Domain (Kcnk) Potassium Channels: Dynamic Roles in Neuronal Function 네이버 미소장
21 Tichenor JN, Hansen ET and Buxton IL. 2005. Expression of stretch-activated potassium channels in human myometrium. Proc. West. Pharmacol. Soc. 48:44-48. 미소장
22 Wareing M, Bai X, Seghier F, Turner CM, Greenwood SL, Baker PN, Taggart MJ and Fyfe GK. 2006. Expression and function of potassium channels in the human placental vasculature. Am. J. Physiol. Regul. Integr. Comp. Physiol. 291: 437-446. 미소장
23 A randomized, controlled trial of magnesium sulfate for the prevention of cerebral palsy. 네이버 미소장
24 Expression of Two-pore Domain K^+ Channels in Endometrial Cells of Korean Cattle 소장