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한국학술지인용색인(NRF)
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재조합 균주인 E. coli JM109/DL-3를 사용하여 carboxymethylcellulase를 생산하기 위한 배지의 최적 염 농도를 orthogonal array method (OAM)과 response surface method (RSM) 등과 같은 통계학적인 방법으로 확립하고 그 결과를 비교하였다. OAM에 기초를 한 Qualitek-4 Software를 사용하여 실험을 계획하고, 그 결과를 분석한 결과는 K_2HPO_4가 균체의 생장 및 carboxymethylcellulase의 생산에 미치는 영향이 가장 크다는 사실을 확인하였다. 균체의 생육에 최적인 K_2HPO_4, NaCl, MgSO_4ㆍ7H_2O 및 (NH_4)_2SO_4의 농도는 10.0, 1.0, 0.2 및 0.6 g/l이었으나, carboxymethylcellulase의 생산에 최적인 각 염들의 농도는 각각 5.0, 1.0, 0.4 및 0.6 g/l이었다. RSM에 기초를 한 Design-Expert Software를 사용하여 실험을 계획하고, 그 결과를 분석한 결과는 K_2HPO_4가 균체의 생장 및 carboxymethylcellulase의 생산에 가장 중요한 인자라는 사실을 확인하였다. 균체의 생장에 최적인 K_2HPO_4, NaCl, MgSO_4ㆍ7H_2O 및 (NH_4)_2SO_4의 농도는 7.44, 1.08, 0.22 및 0.88 g/l이었으나, carboxymethylcellulase의 생산에 최적인 각 염들의 농도는 각각 5.84, 0.69, 0.28 및 0.54 g/l이었다. 기본적으로 OAM에 기초한 software를 사용하여 얻은 결과는 RSM에 기초한 software를 사용하여 얻은 결과와 유사하였다. 최적 조건에서 재조합 균주 E. coli JM109/DL-3이 생산하는 carboxymethylcellulase의 생산은 B. amyloliquifacience DL-에 비하여 1.92배 증가하였다.
The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K_2HPO_4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K_2HPO_4, NaCl, MgSO_4∙7H_2O, and (NH_4)_2SO_4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K_2HPO_4 (“probe>F” less than 0.0001), whereas K_2HPO_4 and MgSO_4ㆍ7H_2O were significant for the production of CMCase. The optimal concentrations of K_2HPO_4, NaCl, MgSO_4ㆍ7H_2O, and (NH_4)_2SO_4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.
The optimal concentrations of salts in medium for cell growth and the production of carboxymethylcellulase (CMCase) by a recombinant E. coli JM109/DL-3 were established using two statistical methods: orthogonal array method (OAM) and response surface method (RSM). The analysis of variance (ANOVA) of data based on OAM indicated that K_2HPO_4 gave maximum sum of square (S) and percentage contribution (P) for cell growth as well as production of CMCase. The optimal concentrations of K_2HPO_4, NaCl, MgSO_4∙7H_2O, and (NH_4)_2SO_4 in medium for cell growth extracted by Qualitek-4 (W32b) Software were 10.0, 1.0, 0.2, and 0.6 g/l, respectively, whereas those for the production of CMCase by E. coli JM109/DL-3 were 5.0, 1.0, 0.4, and 0.6 g/l. The analysis of variance (ANOVA) resulting from RSM indicated that a highly significant salt for cell growth was K_2HPO_4 (“probe>F” less than 0.0001), whereas K_2HPO_4 and MgSO_4ㆍ7H_2O were significant for the production of CMCase. The optimal concentrations of K_2HPO_4, NaCl, MgSO_4ㆍ7H_2O, and (NH_4)_2SO_4 for cell growth extracted by Design Expert Software were 7.44, 1.08, 0.22, and 0.88 g/l, respectively, whereas those for production of CMCase were 5.84, 0.69, 0.28, and 0.54 g/l. The optimal concentrations of salts and their influences on cell growth and production of CMCase extracted by OAM were almost the same as those by RSM. Production of CMCase by a recombinant E. coli JM109/DL-3 under optimized concentration of salts was 1.93 times higher than that by Bacillus amyloliquifaciens DL-3.
권호기사
참고문헌 (28건) : 자료제공( 네이버학술정보 )
| 번호 | 참고문헌 | 국회도서관 소장유무 |
|---|---|---|
| 1 | Medium optimization by orthogonal array designs for urease production by Aspergillus niger PTCC5011  |
미소장 |
| 2 | Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875 |
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| 3 | Biotechnology Journal 3/2008 |
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| 4 | Cui, J. D. 2010. Optimization of medium for phenylalanine ammonia lyase production in E. coli using response surface method. Kor. J. Chem. Eng. 27, 174-178. | 미소장 |
| 5 | Biotechnology Journal 10/2010 |
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| 6 | Gao, W., C. H. Chung, J. Li, and J. W. Lee. 2011. Application of statistical experimental design for optimization of physiological factors and their influences on production of pullulan by Aureobasidium pullulans HP-2001 using an orthogonal array method. Korean J. Chem. Eng. Doi:10.1007/ s11814-011-0107-4. | 미소장 |
| 7 | Characteristics of cellulase preparations affecting the simultaneous saccharification and fermentation of cellulose to ethanol |
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| 8 | Gu, X. B., Z. M. Zheng, H. Q. Yu, J. Wang, F. L. Liang, and R. L. Liu. 2005. Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method. Process Biocehm. 40, 3196-3201. | 미소장 |
| 9 | Optimization of process parameters for key enzymes accumulation of 1,3-propanediol production from Klebsiella pneumoniae |
미소장 |
| 10 | NaCl as a physiological modulator of proline metabolism and antioxidant potential in Phyllanthus amarus |
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| 11 | Biotechnological Approach of Microbial Lipase: A Review |
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| 12 | Biotechnology Journal 3/2008 |
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| 13 | Khuri, A. I. and J. A. Cornell. 1987. Response surfaces: Design and analysis. Marcel Dekker, New York, USA. | 미소장 |
| 14 | Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53 |
미소장 |
| 15 | Industrial scale of optimization for the production of carboxymethylcellulase from rice bran by a marine bacterium, Bacillus subtilis subsp. subtilis A-53 |
미소장 |
| 16 | Lee, N. K., Y. B. Jo, I. H. Jin, C. W. Son, and J. W. Lee. 2009. The effect of potassium phosphate as a pH stabilizer on the production of gellan by Sphingmonas paucibilis NK-2000. J. Life Sci. 19, 1033-1038. | 미소장 |
| 17 | Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull |
미소장 |
| 18 | Response Surface Optimization of the Critical Media Components for the Production of Surfactin |
미소장 |
| 19 | Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design |
미소장 |
| 20 | Biotechnology and bioeconomy in China. |
미소장 |
| 21 | Compactin production in solid-state fermentation using orthogonal array method by P. brevicompactum |
미소장 |
| 22 | Indole-3-Acetic Acid (IAA) Production in Symbiotic and Non-Symbiotic Nitrogen-Fixing Bacteria and its Optimization by Taguchi Design |
미소장 |
| 23 | Sukumaran, R. K., R. R. Singhania, G. M. Mathew, and A. Pandey. 2009. Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production. Renew. Energy 34, 421-424. | 미소장 |
| 24 | Overproduction of recombinant Trichoderma reesei cellulases by Aspergillus oryzae and their enzymatic properties |
미소장 |
| 25 | Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes |
미소장 |
| 26 | Biotechnology in Turkey: an overview. |
미소장 |
| 27 | Optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Paecilomyces tenuipes C240 |
미소장 |
| 28 | Production and Distribution of Endoglucanase, Cellobiohydrolase, and �-Glucosidase Components of the Cellulolytic System of Volvariella volvacea, the Edible Straw Mushroom |
미소장 |
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