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As sucrose has been implicated in healthrelated problems such as obesity and diabetes, awareness of a safe natural non-sugar sweetener has increased. Brazzein is the intensely sweet-tasting plant protein which consists of 54 amino acids. In this study, brazzein was expressed in Lactobacillus spp., probiotics, which are widely used in food industry. Three Lactobacillus spp. (Lb. paracasei KLB58, Lb. plantarum KLB213, and Lb. reuteri KCTC3594) were electrotransformed with pMSP3545::Braht which contains brazzein gene along with nisin-controlled expression (NICE) system. Brazzein expression in Lactobacillus spp. was confirmed by Western blot analysis. Overall, higher expression rate was obtained with nisin concentration of 25-40 ng/mL.

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기사명 저자명 페이지 원문 목차
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Wheat bran globulins : competitive inhibitors of mushroom tyrosinase Angel Martín Ortíz-Estrada, Jorge Nemesio Mercado-Ruiz, Jesús Manuel García-Robles, Alma Rosa Islas-Rubio, Ana María Mendoza-Wilson, René Renato Balandrán-Quintana pp.633-635

Antioxidation activity of oil extracts prepared from various seeds Jin-Won Lee, Young-Teck Kim, Jang-Woo Park, Chul Rhee pp.637-643

Low molecular weight β-glucan stimulates doxorubicin-induced suppression of immune functions in mice Nak-Yun Sung, Eui-Baek Byun, Du-Sup Song, Young-Choon Yoo, Jae-Kyung Kim, Jong-Heum Park, Beom-Seok Song, Sang-Hyun Park, Ju-Woon Lee, Young-Beob Yu … [et al.] pp.645-651

Optimization for the maximum bacteriocin production of Lactobacillus brevis DF01 using response surface methodology Yu Mi Lee, Jun Soo Kim, Wang June Kim pp.653-659

Antioxidant activity of various extracts from an edible mushroom Pleurotus eous Govindan Sudha, Sabapathy Vadivukkarasi, Rajan Babu Indhu Shree, Ponnusamy Lakshmanan pp.661-668

Effect of l-cysteine and lactose on color stability of porcine red blood cell during freeze-drying and powder storage Cunliu Zhou, Hui Wang, Yunlong Chen, Conggui Chen pp.669-674

Physicochemical and nutritional properties of spray-dried pitaya fruit powder as natural colorant Ng Lay Tze, Chong Pik Han, Yus Aniza Yusof, Chin Nyuk Ling, Rosnita A. Talib, Farah Saleena Taip, Mohammad Gulzarul Aziz pp.675-682

Anti-proliferative properties of ethyl acetate extract of Phellinus linteus grown on ginseng(Panax ginseng) on HT-29 human colon carcinoma cells through inducing apoptosis Dong Ki Park, Hye-Jin Park pp.683-690

Effects of yeast extract and different amino acids on the dynamics of some components in cabbage juice during fermentation with Bifidobacterium lactis BB-12 Claudia Lavinia Buruleanu, Carmen Leane Nicolescu, Daniela Avram, Iuliana Manea, Magda Gabriela Bratu pp.691-699

Improved functionality of soft soybean curd containing Monascus fermented soybean ethanol extract Sung Hyun Hong, Inhyung Lee, Seok Joong Kim, Jee-Young Imm pp.701-707

Clarification, preservation, and shelf life evaluation of cashew pple juice Uma Talasila, Rama Rao Vechalapu, Khasim Beebi Shaik pp.709-714

Comparative study of antioxidant effects of five Korean varieties red pepper(Capsicum annuum L) extracts from various parts including placenta, stalk, and pericarp Lei Chen, Ji-Eun Hwang, Kang Mo Gu, Jung-Hyun Kim, Boram Choi, Kyung-Sik Song, Youmie Park, Young-Hwa Kang pp.715-721

Inhibitory effect of sowthistle(Ixeris dentata) on development and expression of behavioral locomotor sensitization to nicotine in rats Bombi Lee, Jinhee Park, Sunoh Kwon, Mijung Yeom, Bongjun Sur, Insop Shim, Hyejung Lee, Suk Hoo Yoon, Dong-Myong Jeong, Dae-Hyun Hahm pp.723-729

Cloning and expression of β-glucosidases from Bifidobacterium lactis AD011 Jin Yong Kim, Yan Wang, Su Ji Park, Geun Eog Ji, Myeong Soo Park pp.731-738

Growth stimulation/inhibition effect of medicinal plants on human intestinal microbiota Atchara Nuphet Phoem, Supayang Piyawan Voravuthikunchai pp.739-745

Morphological, structural, and functional properties of maranta(Maranta arundinacea L) starch Madhava Naidu Madineni, Sheema Faiza, Ragu Sai Surekha, Ramasamy Ravi, Manisha Guha pp.747-752

Seasonal influence on phenolic-mediated antihyperglycemic properties of Canadian sugar and red maple leaves using in vitro assay models Emmanouil Apostolidis, Liya Li, Bouhee Kang, Chong M. Lee, Navindra P. Seeram pp.753-760

Antioxidant, tyrosinase inhibitory, and acetylcholinesterase inhibitory activities of green tea(Camellia sinensis L.) seed and its pericarp Young-Hong Jo, Hyun-Gyun Yuk, Jong-Hwa Lee, Jong-Cheol Kim, Rumi Kim, Seung-Cheol Lee pp.761-768

Effect of hydrocolloids on the pasting and rheological characteristics of resistant starch(type 4) Kwang Yeon Lee, Hua Yao, In Young Bae, Suyong Lee, Hyeon Gyu Lee pp.769-774

Inhibitory effects of antagonistic compounds produced from Lactobacillus brevis MLK27 on adhesion of Listeria monocytogenes KCTC3569 to HT-29 cells Sung-Mee Lim, Dong-Soon Im pp.775-784

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Optimization of extraction of bioactive alkaloids and antioxidants from rhizome coptidis(Coptis Chinensis Franch) by using response surface methodology Hui Teng, Yong Hee Choi pp.799-806

Perilla oil rich in α-linolenic acid suppresses hepatic SREBPs and NF-κB expression in hypercholesterolemia-induced apolipoprotein E knockout mice Jieun Lee, Yeong Ok Song pp.807-813

Effect of heat treatment condition on the antioxidant and several physiological activities of non-astringent persimmon fruit juice Dong-Won Lee, Seung-Cheol Lee pp.815-822

Duplex polymerase chain reaction method for detection of unapproved genetically modified tomato(Solanum lycopersicon L.) with cucumber mosaic virus(CMV) satellite RNA gene Jae-Hwan Kim, Jae-Eun Lee, Hae-Yeong Kim pp.823-827

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Whole cell biotransformation of major ginsenosides using Leuconostocs and Lactobacilli Su Ji Park, So Youn Youn, Geun Eog Ji, Myeong Soo Park pp.839-844

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Inter-laboratory study to define the temperature interval for a thermoluminescence heating unit used to identify irradiated food Jae-Jun Ahn, Kashif Akram, Ji-Yeong Baek, Yeon-Joo Kim, Moon-Young Kim, Il-Yun Jeong, Joong-Ho Kwo pp.853-857

Modeling the combined effect of temperature and relative humidity on Escherichia coli O157:H7 on lettuce Jun Wang, Jeanne-Marie Membré, Sang-Do Ha, Gyung-Jin Bahk, Myung-Sub Chung, Hyang-Sook Chun, In-Gyun Hwang, Deog-Hwan Oh pp.859-865

Enhancing citric acid production of Yarrowia lipolytica by mutagenesis and using natural media containing carrot juice and celery byproducts Seda Karasu Yalcin pp.867-874

Effect of combined extract of Hansogdan(Phlomis umbrosa) and Dalgaebi(Commelina communis) as a milk additive for enhancing the growth of physical height in vivo Jae Kyung Lee, Dong-guk Lee, Bo-Yeon Kwak, Jae Soo Kim, Kwontaek Yi pp.875-879

Targeted phenolic analysis in Hericium erinaceum and its antioxidant activities Hua Li, Sol Park, BoKyoung Moon, Young-Bok Yoo, Young-Wook Lee, Chan Lee pp.881-888

Relative sweetness and sensory characteristics of bulk and intense sweeteners Mi-Jin Gwak, Seo-Jin Chung, Young Jae Kim, Chun Sun Lim pp.889-894

Expression of the sweet-tasting protein brazzein in Lactobacillus spp. Yoo-Won Lee, Kwan-Young Kim, Sang-Hyun Han, Chang-Ho Kang, Jae-Seong So pp.895-898

Effect of high pressure processing on microbiological and physical qualities of carrot and spinach Lae-Seung Jung, Seung Hwan Lee, Sungkyun Kim, Sung-Ki Lee, Juhee Ahn pp.899-904

Antibacterial properties of honey produced by Melipona beecheiiand Apis mellifera against foodborn microorganisms David Chan-Rodríguez, Jesús Ramón-Sierra, Jorge Lope-Ayora, Enrique Sauri-Duch, Luis Cuevas-Glory, Elizabeth Ortiz-Vázquez pp.905-909

Differentiation of lactic acid bacteria based on RFLP analysis of the tuf gene Seok-Hwee Park, Jong-Hyun Jung, Dong-Ho Seo, Hye-Lim Lee, Gye-Won Kim, Sun-Young Park, Woo-Chang Shin, Sungyoul Hong, Cheon-Seok Park pp.911-915

In vivo imaging of Escherichia coli and Lactococcus lactis in murine intestines using a reporter luciferase gene Mi-Sook Lee, Gi-Seong Moon pp.917-920

참고문헌 (18건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 National Cancer Institute Fact Sheet. Artificial sweeteners and cancer: Questions and answers. Available from: http://www.cancer. gov/cancertopics/factsheet/Risk/artificial-sweeteners#ref1. Accessed Jan. 7, 2010. 미소장
2 Aspartame induces lymphomas and leukaemias in rats 네이버 미소장
3 Recent developments in the characterization and biotechnological production of sweet-tasting proteins. 네이버 미소장
4 Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. 네이버 미소장
5 Efficient Production of Recombinant Brazzein, a Small, Heat-Stable, Sweet-Tasting Protein of Plant Origin 네이버 미소장
6 Berlec A, Jevnikar Z, Majhenic AC, Rogelj I, Strukelj B. Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: A path toward sweet lactic acid bacteria. Appl. Microbiol. Biot. 73: 158-165 (2006) 미소장
7 Plant Biotech 네이버 미소장
8 Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. 네이버 미소장
9 Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis. 네이버 미소장
10 Large increase in brazzein expression achieved by changing the plasmid /strain combination of the NICE system in Lactococcus lactis. 네이버 미소장
11 Food Production and Food Safety 네이버 미소장
12 Complete Genome Sequence of Lactobacillus plantarum WCFS1 네이버 미소장
13 An improved method for the transformation of Lactobacillus strains using electroporation 네이버 미소장
14 Biotechnology in Turkey: an overview. 네이버 미소장
15 Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, New York, NY, USA (1989) 미소장
16 Adaptation of the nisin-controlled expression system in Lactobacillus plantarum: a tool to study in vivo biological effects. 네이버 미소장
17 Chang CE, Pavlova SI, Tao L, Kim EK, Kim SC, Yun HS, So JS. Molecular identification of vaginal Lactobacillus spp. isolated from Korean women. J. Microbiol. Biotechn. 12: 312-317 (2000) 미소장
18 Improved Vectors for Nisin-Controlled Expression in Gram-Positive Bacteria 네이버 미소장

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