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Although offline enrichment of phosphorylated peptides is widely used, enrichment for phosphopeptides using TiO₂ is often performed manually, which is labor-intensive and can lead to irreproducible results. To address the problems associated with offline enrichment and to improve the effectiveness of phosphopeptide detection, we developed an automated online enrichment system for phosphopeptide analysis. A standard protein mixture comprising BSA, fetuin, crystalline, α-casein and β-casein, and ovalbumin was assessed using our new system. Our multidimensional system has four main parts: a sample pump, a 20-mm TiO₂-based column, a weak anionexchange, and a strong cation-exchange (2:1 WAX:SCX) separation column with LC/MS. Phosphorylated peptides were successfully detected using the TiO₂-based online system with little interference from nonphosphorylated peptides. Our results confirmed that our online enrichment system is a simple and efficient method for detecting phosphorylated peptides.

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권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Characterization of human cytosolic thioredoxin mutants with increasing side chain volumes at residue-33 Lin Yuan, Young-Joon Cho, Hakjung Kim pp.3169-3170

Organocatalytic asymmetric conjugate addition of 3-alkyl-substituted oxindoles to vinyl ketones Hyun Joo Lee, Dae Young Kim pp.3171-3172

Anefficient synthesis of nitriles from aldoximes using diethyl phosphorocyanidate under mild conditions Kieseung Lee, Hyeon-Seong An, Chan-Yeon Hwang pp.3173-3174

Organocatalytic and enantioselective mannich reaction of dicyanoolefins with α-amido sulfones Jun Lu, Wei-Yi Chen pp.3175-3176

Synthesis of carbon nanofibers based on resol type phenol resin and Fe(III) catalysts Yura Hyun, Haesik Kim, Chang-Seop Lee pp.3177-3183

Cationic dye(methylene blue) removal from aqueous solution by montmorillonite Baybars Ali Fil, Cengiz Özmetin, Mustafa Korkmaz pp.3184-3190

Fabrication of amino acid based silver nanocomposite hydrogels from PVA- poly(acrylamide-co-acryloyl phenylalanine) and their antimicrobial studies Hyeong-Rae Cha, V. Ramesh Babu, K.S.V. Krishna Rao, Yong-Hyun Kim, Surong Mei, Woo Hong Joo, Yong-Ill Lee pp.3191-3195

Effect of template removal on synthesis of organic-inorganic hybrid mesoporous MCM-48 Ya Nan Zhao, San Xi Li, Chong Soo Han pp.3196-3202

Kinetics and mechanism of the pyridinolysis of diisopropyl chlorothiophosphate in acetonitrile Md. Ehtesham Ul Hoque, Hai Whang Lee pp.3203-3207

Development of SS-AG20-loaded polymeric microparticles by oil-in-water(o/w) emulsion solvent evaporation and spray drying methods for sustained drug delivery Eunjung Choi, Cheng Zhe Bai, Areum Hong, Jong-Sang Park pp.3208-3212

Ni/ZnO-based adsorbents supported on Al₂O₃, SiO₂, TiO₂, ZrO₂ : a comparison for desulfurization of model gasoline by reactive adsorption Xuan Meng, Huan Huang, Huixin Weng, Li Shi pp.3213-3217

One pot synthesis and characterization of alginate stabilized semiconductor nanoparticles Parani Sundarrajan, Prabakaran Eswaran, Alexander Marimuthu, Lakshmi Baddireddi Subhadra, Pandian Kannaiyan pp.3218-3224

Synthesis and physicochemical characterization of biodegradable PLGA-based magnetic nanoparticles containing amoxicil[l]in Somayeh Alimohammadi, Roya Salehi, Niloofar Amini, Soodabeh Davaran pp.3225-3232

Protein analysis using a combination of an online monolithic trypsin immobilized enzyme reactor and collisionally-activated dissociation/electron transfer dissociation dual tandem mass spectrometry Hyo Jin Hwang, Kun Cho, Jin Young Kim, Young Hwan Kim, Han Bin Oh pp.3233-3240

Ionic liquids as benign solvents for the extraction of aromatics Md. Anwar Hossain, Jeesun Lee, Dai Hyun Kim, Dinh Quan Nguyen, Minserk Cheong, Hoon Sik Kim pp.3241-3247

¹H, ^15^N and ^13^C backbone assignments and secondary structures of C-ter100 domain of vibrio extracellular metalloprotease derived from Vibrio vulnificus Ji-Hye Yun, Heeyoun Kim, Jung Eun Park, Hae-kap Cheong, Chaejoon Cheong, Jung Sup Lee, Weontae Lee pp.3248-3252

Kinetic study on nucleophilic substitution reactions of phenyl Y-substituted-phenyl carbonates with Z-substituted-phenoxides : effect of modification of nonleaving group from benzoyl to phenyloxycarbonyl on reactivity and reaction mechanism Se-Won Min, Min-Young Kim, Ik-Hwan Um pp.3253-3257

Effects of surface nitrification on thermal conductivity of modified aluminum oxide nanofibers-reinforced epoxy matrix nanocomposites Byung-Joo Kim, Kyong-Min Bae, Kay-Hyeok An, Soo-Jin Park pp.3258-3264

Comparative toxicity studies of ultra-pure Ag, Au, Co, and Cu nanoparticles generated by laser ablation in biocompatible aqueous solution Yea Seul Kim, Kuk Ki Kim, Seonmi Shin, Seung Min Park, Sang Soo Hah pp.3265-3268

High-contrast imaging of biomolecular interactions using liquid crystals supported on roller printed protein surfaces Min-Kyung Park, Chang-Hyun Jang pp.3269-3273

Kinetics and mechanism of anilinolysis of phenyl N-phenyl phosphoramidochloridate in acetonitrile Md. Ehtesham Ul Hoque, Hai Whang Lee pp.3274-3278

Synthesis and characterization of H₃PO₄doped poly(benzimidazole-co-benzoxazole) membranes for high temperature polymer electrolyte fuel cells Hye-Jin Lee, Dong Hoon Lee, Dirk Henkensmeier, Jong Hyun Jang, Eun Ae Cho, Hyoung-Juhn Kim, Hwayong Kim pp.3279-3284

Theoretical study of thiazole adsorption on the (6,0) zigzag single-walled boron nitride nanotube Ali Varasteh Moradi, Ali Ahmadi Peyghan, Saeede Hashemian, Mohammad T. Baei pp.3285-3292

Rate-product correlations for the solvolysis of 5-nitro-2-furoyl chloride Hojune Choi, Han Joong Koh, Dildar Ali, Kiyull Yang, In Sun Koo pp.3293-3297

Amultidimensional system for phosphopeptide analysis using TiO₂enrichment and ion-exchange chromatography with mass spectrometry Kun Cho, Jisun Yoo, Eunmin Kim, Jin Young Kim, Young Hwan Kim, Han Bin Oh, Jong Shin Yoo pp.3298-3302

Single-crystal structures of Li⁺-exchanged zeolite X(FAU, Si/Al = 1.09) from aqueous solution depends on ion-exchange temperatures at 293 and 333 K Hu Sik Kim, Seong Oon Ko, Woo Taik Lim pp.3303-3310

Extraction of phthalate esters in environmental water samples using layered-carbon magnetic hybrid material as adsorbent followed by their determination with HPLC Weina Wang, Qiuhua Wu, Xiaohuan Zang, Chun Wang, Zhi Wang pp.3311-3316

Synthesis, structure and thermal properties of bifurazano[3,4-b:3',4'-f]furoxano[3'',4''-d]oxacyclohetpatriene(BFFO) Yanshui Zhou, Kangzhen Xu, Bozhou Wang, Hang Zhang, Qianqian Qiu, Fengqi Zhao pp.3317-3320

mPW1PW91 study of complexation of alkali metal ions with mixed [2 + 2'] calix[4]aryl derivatives Jong-In Choe pp.3321-3330

Particle size effect : Ru-modified Pt nanoparticles toward methanol oxidation Sechul Kim, Ting Zhang, Jin-Nam Park, Choong Kyun Rhee, Hojin Ryu pp.3331-3337

ADFT study on CO₂interaction with a BN nano-cage Mohammad T. Baei, Ali Ahmadi Peyghan, Zargham Bagheri pp.3338-3342

DFT/B3LYP study to investigate the possible ways for the synthesize of antioxidants with high efficiency based on vitamin E Meysam Najafi, Mohammad Najafi, Houshang Najafi pp.3343-3348

Impedance and thermodynamic analysis of bioanode, abiotic anode, and riboflavin-amended anode in microbial fuel cells Sokhee Jung, Young-Ho Ahn, Sang-Eun Oh, Junho Lee, Kyu Taek Cho, Youngjin Kim, Myeong Woon Kim, Joonmok Shim, Moonsung Kang pp.3349-3354

Photovoltaic efficiencies on dye-sensitized solar cells assembled with graphene-linked TiO₂anode films A-Young Kim, Jieun Kim, Min Young Kim, Seung Won Ha, Nguyen Thi Thuy Tien, Misook Kang pp.3355-3360

Synthesis, antioxidant activity and fluorescence properties of novel europium complexes with (E)-2- or 4-hydroxy-N'-[(2-hydroxynaphthalen-1-yl)methylene]benzohydrazide schiff base Lijun Liu, Mohammad Sayed Alam, Dong-Ung Lee pp.3361-3367

Enhanced gas sensing properties of Bi₂O₃-core/In₂O₃-shell nanorod gas sensors Sunghoon Park, Soyeon An, Hyunsung Ko, Changhyun Jin, Chongmu Lee pp.3368-3372

Reusable and efficient polystryrene[i.e. polystyrene]-supported acidic ionic liquid catalyst for mononitration of aromatic compounds Li Xia Li, Qi Long Ling, Zu Liang Liu, Xiao Dong Xing, Xiao Qin Zhu, Xiao Meng pp.3373-3377

Fabrication of a superhydrophobic surface with adjustable hydrophobicity and adhesivity based on a silica nanotube array Jaeeun Yu, Sang Jun Son pp.3378-3382

Preparation of SnS thin films by MOCVD method using single source precursor, Bis(3-mercapto-1-propanethiolato) Sn(II) Jongpil Park, Miyeon Song, Won Mok Jung, Won Young Lee, Jinho Lee, Hanggeun Kim, Il-Wun Shim pp.3383-3386

Separation of two amino acids by microemulsion bulk liquid membrane Alireza Salabat, Fereshteh Dehghani Sanij pp.3387-3390

Extracting frequency-frequency correlation function from two-dimensional infrared spectroscopy : peak shift measurement Kyung-Won Kwak pp.3391-3396

Growth mechanism and crystal ordering of spherulitic patterns in a Belousov-Zhabotinsky type reaction system Narendra Yadav, S.S. Majhi, P.K. Srivastava pp.3397-3406

Improved photolysis of water from Ti incorporated double perovskite Sr₂FeNbO₆ lattice P.H. Borse, C.R. Cho, S.M. Yu, J.H. Yoon, T.E. Hong, J.S. Bae, E.D. Jeong, H.G. Kim pp.3407-3412

Synthesis of an environmentally friendly phenol-free resin for printing ink Young Baeck Ha, Ming Yu Jin, Sung Sang Oh, Do Hyun Ryu pp.3413-3416

Synthesis and antiviral activity of 3-aminoindole nucleosides of 2-acetamido-2-deoxy-D-glucose Adel A.-H. Abdel-Rahman, Mona M. Abd El-Latif, Farag A. El-Essawy, Yousif A. Barakat pp.3417-3422

Dielectric study of allyl chloride with 2-pentanone and 2-hexanone in microwave frequency range Yuvraj Sudake, Siddharth Kamble, Aruna Maharolkar, Sunil Patil, Prakash Khirade, Suresh Mehrotra pp.3423-3426

Synthesis and structural determination of temocapril sulfoxide hydrochlorides Seok Bong Seong, Jong Taik Moon, Jungahn Kim, Dong Joon Choo, Jae Yeol Lee pp.3427-3429

N-heterocyclic carbene-tricyclohexylphosphine palladium(II) complex : synthesis, crystal structure and application in Suzuki reaction of aryl chlorides Chen Xu, Xin-Qi Hao, Zhen Li, Lu-Meng Duan, Zhi-Qiang Wang, Bao-Ming Ji, Mao-Ping Song pp.3430-3432

Red fluorescent DCM derivatives with the bulky-substituents on pyran and julolidine moieties for organic light-emitting diodes(OLEDs) Kum Hee Lee, Young Kwan Kim, Seung Soo Yoon pp.3433-3436

Pyridinolysis of phenyl N-phenyl phosphoramidochloridate in acetonitrile Md. Ehtesham Ul Hoque, Hai Whang Lee pp.3437-3440

Pyridinolysis of dipropyl chlorophosphate in acetonitrile Md. Ehtesham Ul Hoque, Hai Whang Lee pp.3441-3444

New titanium-based catalysts for the synthesis of poly(ethylene terephthalate) Young-Keun Yang, Seung-Woong Yoon, Yong-Taek Hwang, Bo-Geun Song pp.3445-3447

Methanolysis of 7,7,8,8-tetracyanoquinodimethane : a consequence of an initial charge transfer complexation Usama M. Rabie pp.3448-3450

Synthesis of dendrimer containing carbazole unit as a core chromophore Seung Choul Han, Sung-Ho Jin, Jae Wook Lee pp.3451-3454

Two new alkaloids from the rhizomes of Sinomenium acutum Seung Young Lee, Il Kyun Lee, Jong Eel Park, Ju Yeoun Oh, Kang Ro Lee pp.3455-3457

Immobilization of enzyme on hydrophobic room temperature solid-phase ionic liquids and its use for biocatalytic transesterification in organic solvent : remarkably enhanced activity of lipase Jae Kwan Lee pp.3458-3460

Chemical constituents of the Ficus elastica leaves and their antioxidant activities Phan Van Kiem, Chau Van Minh, Nguyen Xuan Nhiem, Bui Huu Tai, Tran Hong Quang, Hoang Le Tuan Anh, Nguyen Xuan Cuong, Truong Nam Hai, Seung Hyun Kim, Jin Kyoung Kim … [et al.] pp.3461-3464

3,9-dithia-6-azaundecane-appended iridium(III) complex for the selective detection of Hg^2+^ in aqueous acetonitrile Jee Hye Ann, Yinan Li, Myung Ho Hyun pp.3465-3468

New Si-based material with pyridopyrazine substituents So Min Park, Il Kang, Hui Jun Yun, Ran Kim, Sang Young Nam, Jaeyoung Hwang, Yun-Hi Kim pp.3469-3472

CdSe nanowire/fullerene nanocomposite photoelectrode : charge separation and photoelectrochemical behavior Ayoung Kim, Jihee Lee, Eun Joo Lee, Jin Ho Bang pp.3473-3476

Photodecarboxylative cyclizations of ω-phthalimido-para-phenoxy carboxylates Ae Rhan Kim, Hyun-Seung Cho, Youn-Sik Lee, Dong Jin Yoo pp.3477-3480

Comparative studies on enantiomer resolution of α-amino acids and their esters using (18-crown-6)-tetracarboxylic acid as a chiral crown ether selector by NMR spectroscopy and high-performance liquid chromatography Eunjung Bang, Jing Yu Jin, Joon Hee Hong, Jong Seong Kang, Weontae Lee, Wonjae Lee pp.3481-3484

Optimization of solvent for the determination of polybrominated diphenyl ethers in high-impact polystyrene by GC/ECD Dalho Kim, Seungjin Park, Yongwook Choi, Sung Kwang Lee pp.3485-3488

Facile synthesis of BaWO₄sub-micron sized octahedron via a microemulsion method Kun Kim, Young-Duk Huh pp.3489-3492

Anomalous acid-base equilibria in biologically relevant water nanopools Sun-Young Park, Byung Kuk Yoo, Ji-Young Pyo, Myung Soo Kim, Du-Jeon Jang pp.3493-3496

Enantiomeric separation of 1-(benzofuran-2-yl)alkylamines on chiral stationary phases based on chiral crown ethers Soohyun Park, Sang Jun Kim, Myung Ho Hyun pp.3497-3500

Convergent synthesis of PAMAM dendrimers containing tetra(ethyleneoxide) at core using click chemistry Seung Choul Han, Ji Hyeon Kim, Jae Wook Lee pp.3501-3504

Inhibition of DUSP13B activity by NSC 663284 In-Seon Lee, Anna Ju, Sayeon Choe pp.3505-3507

참고문헌 (39건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
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