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Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the cryopreservation procedure might also be error-prone. However, only limited amounts of material are available for analysis. To improve the monitoring of frozen/thawed spermatozoa, commonly used in vitro fertilization (IVF) followed by embryo transfer were replaced with animal-free techniques. Major factors for assessing spermatozoa quality (i.e., density, viability, motility, and morphology) were evaluated by fluorescence microscopy. For this, a live/dead cell staining protocol requiring only small amounts of material was created. Membrane integrity was then examined as major parameter closely correlated with successful IVF. These complex analyses allow us to monitor frozen/thawed spermatozoa from GM mice using a relatively simple staining procedure. This approach leads to a reduction of animal experiments and contributes to the 3R principles (replacement, reduction and refinement of animal experiments).

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기사명 저자명 페이지 원문 목차
Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs Zhang-Yong Ning, Xin-Tao Wu, Yan-Fen Cheng, Wen-Bao Qi, Yu-Fu An, Heng Wang, Gui-Hong Zhang, Shou-Jun Li pp.219-222

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogentreated male Japanese quails(Coturnix coturnix japonica) Shinji Hiyama, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida pp.223-227

Theeffect of melanocortin(Mc3 and Mc4) antagonists on serotonin-induced food and water intake of broiler cockerels Morteza Zendehdel, Farshid Hamidi, Vahab Babapour, Kasra Mokhtarpouriani, Ramin Mazaheri Nezhad Fard pp.229-234

Activation of Akt/protein kinase B mediates the protective effects of mechanical stretching against myocardial ischemia-reperfusion injury Chan-Hyung Kim, Jia Hao, Hee-Yul Ahn, Si Wook Kim pp.235-244

Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice Hsien Yueh Liu, Chih-Yao Chung, Wen-Chin Yang, Chih-Lung Liang, Chi-Young Wang, Chih-Yu Chang, Cicero Lee-Tian Chang pp.245-252

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao, Jörg Hartung pp.253-259

Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements Laureano S. Frizzo, María V. Zbrun, Lorena P. Soto, Ezequiel Bertozzi, Gabriel J. Sequeira, Luis E. Marti, Marcelo L. Signorini, Roberto Rodríguez Armesto, Marcelo R. Rosmini pp.261-270

Frequency of group A rotavirus with mixed G and P genotypes inbovines : predominance of G3 genotype and its emergence in combination with G8/G10 types Yashpal S. Malik, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K.M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Minakshi, Gaya Prasad, Baldev R. Gulati … [et al.] pp.271-278

Prevalence of peste des petits ruminants among sheep and goats in India Vinayagamurthy Balamurugan, Paramasivam Saravanan, Arnab Sen, Kaushal Kishor Rajak, Gnanavel Venkatesan, Paramanandham Krishnamoorthy, Veerakyathappa Bhanuprakash, Raj Kumar Singh pp.279-285

Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice Jeong Ju Lim, Dong Hyeok Kim, Jin Ju Lee, Dae Geun Kim, Wongi Min, Hu Jang Lee, Man Hee Rhee, Suk Kim pp.287-292

Theeffect of seasonal variation on anthrax epidemiology in the upper Zambezi floodplain of western Zambia Hetron Mweemba Munang'andu, Fredrick Banda, Victor Mukulule Siamudaala, Musso Munyeme, Christopher Jacob Kasanga, Byman Hamududu pp.293-298

Comparing the osteogenic potential of canine mesenchymal stem cells derived from adipose tissues, bone marrow, umbilical cord blood, and Wharton's jelly for treating bone defects Byung-Jae Kang, Hak-Hyun Ryu, Sung Su Park, Yoshihisa Koyama, Masanori Kikuchi, Heung-Myong Woo, Wan Hee Kim, Oh-Kyeong Kweon pp.299-310

Contrast-enhanced ultrasound analysis of renal perfusion in normal micropigs Kangjae Yi, Seoyeoun Ji, Junyoung Kim, Junghee Yoon, Mincheol Choi pp.311-314

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy Ann-Kathrin Diercks, Heinrich F. Bürgers, Anna Schwab, Johannes Schenke pp.315-322

Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR Pam Dachung Luka, Chrisostom Ayebazibwe, David Shamaki, Frank Norbert Mwiine, Joseph Erume pp.323-325

Use of canine small intestinal submucosa allograft for treating perineal hernias in two dogs A-Jin Lee, Wook-Hun Chung, Dae-Hyun Kim, Kyung-Pil Lee, Hyun Jung Suh, Sun Hee Do, Ki-dong Eom, Hwi-Yool Kim pp.327-330

참고문헌 (33건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Comparison of different methods for assessment of sperm concentration and membrane integrity with bull semen. 네이버 미소장
2 Cryopreservation of domestic animal sperm cells. 네이버 미소장
3 Flow Cytometric Characterization of Viable Meiotic and Postmeiotic Cells by Hoechst 33342 in Mouse Spermatogenesis 네이버 미소장
4 Development of mouse embryos in vitro is affected by strain and culture medium. 네이버 미소장
5 Environmental influences on the production of pre-implantation embryos 네이버 미소장
6 Draper NR, Smith H. Applied Regression Analysis. 2nd ed.pp. 43-47, John Wiley & Sons, New York, 1981. 미소장
7 On the Interpretation of χ2from Contingency Tables, and the Calculation of P 네이버 미소장
8 Comparison of Post‐Thaw DNA Integrity of Boar Spermatozoa Assessed with the Neutral Comet Assay and Sperm‐Sus Halomax Test Kit 네이버 미소장
9 Assessment of semen quality, sperm cryopreservation and heterologous IVF in the critically endangered Iberian lynx (Lynx pardinus). 네이버 미소장
10 The Sampling Distribution of the Coefficient of Variation 네이버 미소장
11 Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression. 네이버 미소장
12 Hollander M, Wolfe DA. Nonparametric StatisticalMethods. 1st ed. pp. 191-192, John Wiley & Sons, NewYork, 1973. 미소장
13 The use of flow cytometry in the evaluation of cell viability of cryopreserved sperm of the marine shrimp ( Litopenaeus vannamei) 네이버 미소장
14 Nagy A, Gertsenstein M, Vintersten K, Behringer R.Manipulating the Mouse Embryo: a Laboratory Manual. 3rded. pp. 155-156, Cold Spring Harbor Laboratory Press, NewYork, 2003. 미소장
15 Nagy A, Gertsenstein M, Vintersten K, Behringer R.Manipulating the Mouse Embryo: a Laboratory Manual. 3rded. pp. 570-572, Cold Spring Harbor Laboratory Press, NewYork, 2003. 미소장
16 Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units. 네이버 미소장
17 Ostermeier GC, Wiles MV, Farley JS, Taft RA.Conserving, distributing and managing genetically modifiedmouse lines by sperm cryopreservation. PLoS One 2008, 3,e2792. 미소장
18 Evaluation of fresh and frozen-thawed fowl semen by flow cytometry 네이버 미소장
19 Determinants of sperm quality and fertility in domestic species. 네이버 미소장
20 Russell WMS, Burch RL. The Principles of HumaneExperimental Technique. pp. 69-153, Methuen, London,1959. 미소장
21 Assessment of sperm quality, DNA integrity and cryopreservation protocols in men diagnosed with testicular and systemic malignancies. 네이버 미소장
22 Collection, cryopreservation, storage, and revitalization of transgenic mouse embryos. 네이버 미소장
23 Cryopreservation of Mouse Spermatozoa 네이버 미소장
24 Development in vitro of preimplantation embryos from 55 mouse strains. 네이버 미소장
25 In vitro fertilization with cryopreserved inbred mouse sperm. 네이버 미소장
26 Motility of Cryopreserved Mouse Spermatozoa Affected by Temperature of Collection and Rate of Thawing 네이버 미소장
27 Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol. 네이버 미소장
28 Osmotic tolerance of mouse spermatozoa from various genetic backgrounds: Acrosome integrity, membrane integrity, and maintenance of motility 네이버 미소장
29 Fundamental cryobiology of reproductive cells and tissues 네이버 미소장
30 Acrosome reaction of mouse epididymal sperm on oocyte zona pellucida. 네이버 미소장
31 Fresh and frozen–thawed sperm quality, nuclear DNA integrity, invitro fertility, embryo development, and live-born offspring of N-ethyl- N-nitrosourea (ENU) mice 네이버 미소장
32 Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse. 네이버 미소장
33 Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components. 네이버 미소장

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