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Interferon induced transmembrane protein-1 (Ifitm-1) has been reported to have an important role in primordial germ cell formation, and it has expressed in female reproductive organ. In the present study, Ifitm-1 gene expression was identified in testes and all part of epididymis using western immunoblot and immunohistochemistry. Interestingly,Ifitm-1 expression was observed on the head of spermatozoa. To investigate the role of Ifitm-1 gene expression in behavior of spermatozoa after acrosome reaction, fresh sperm was incubated with calcium ionophore to induce acrosome reaction, whereas the expression of Ifitm-1 was not altered after the acrosome reaction. Then to identify the effect of Ifitm-1 in sperm motility and other seminal parameters, different concentration of Ifitm-1 antibody was incubated with spermatozoa, and seminal parameters were assessed using computer-assisted semen analysis (CASA).

Interestingly, motility, progressive, and VAP were increased in the sperm with Ifitm-1 antibody treated compared to rabbit serum, however other parameters such as straightness were not changed. In order to identify the functional significance of Ifitm-1 in fertilization, capacitated spermatozoa were pre-incubated with anti- Ifitm-1 antibody and subsequently examined the ability to adhere to mouse oocytes. However, any defection or alteration in sperm-egg fusion was not found, Ifitm-1 antibody treated or non-treated spermatozoa showed a normal penetration. Although the precise role of Ifitm-1 in sperm motility and following fertilization need to be elucidated, this study suggests that the activation of Ifitm-1 on the sperm may enhance the motility of spermatozoa in mice.

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참고문헌 (19건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 Abou-Haila A, Tulsiani DR (2000): Mammalian sperm acrosome: formation, contents, and function. Arch Biochem Biophys 379:173-182. 미소장
2 Expression of IFITM1 in chronic myeloid leukemia patients. 네이버 미소장
3 Seminiferous tubule degeneration and infertility in mice with sustained activation of WNT/CTNNB1 signaling in sertoli cells. 네이버 미소장
4 The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. 네이버 미소장
5 Töpfer-Petersen E, Petrounkina AM, Ekhlasi-Hundrieser M (2000): Oocyte-sperm interactions. Ani reprod sci 60-61:653-662. 미소장
6 IFN-alpha induces homotypic adhesion and Leu-13 expression in human B lymphoid cells. 네이버 미소장
7 Monoclonal antibody to the interferon-inducible protein Leu-13 triggers aggregation and inhibits proliferation of leukemic B cells. 네이버 미소장
8 Fumoto S, Shimokuni T, Tanimoto K, Hiyama K, Otani K, Ohtaki M, Hihara J, Yoshida K, Hiyama E, Noguchi T, Nishiyama M (2008): Selection of a novel drug-response predictor in esophageal cancer: a novel screening method using microarray and identification of IFITM1 as a potent marker gene of CDDP response. Int J Oncol 32:413-23. 미소장
9 Molecular mechanisms of male germ cell differentiation 네이버 미소장
10 Dissecting Wnt/beta-catenin signaling during gastrulation using RNA interference in mouse embryos. 네이버 미소장
11 In vivo and in vitro differentiation of male germ cells in the mouse. 네이버 미소장
12 Characterisation of mouse interferon-induced transmembrane protein-1 gene expression in the mouse uterus during the oestrous cycle and pregnancy. 네이버 미소장
13 (b) Park HJ (2011): Characterization and gene expression study of mouse interferon-induced transmembrane protein-1. PhD Thesis. Konkuk University. 미소장
14 A monoclonal antibody that induces T cell aggregation reacts with vascular endothelial cells and placental trophoblasts. 네이버 미소장
15 Saitou M, Payer B, Lange UC, Erhardt S, Barton SC, Surani MA (2003): Specification of germ cell fate in mice. Philos Trans R Soc Lond B Biol Sci 358:1363- 1370. 미소장
16 Tanaka SS, Matsui Y (2002): Developmentally regulated expression of mil-1 and mil-2, mouse interferon- induced transmembrane protein like genes, during formation and differentiation of primodial germ cells. Gene Expr Patterns 2:297-303 미소장
17 IFITM/Mil/Fragilis Family Proteins IFITM1 and IFITM3 Play Distinct Roles in Mouse Primordial Germ Cell Homing and Repulsion 네이버 미소장
18 IFITM1 plays an essential role in the antiproliferative action of interferon-γ 네이버 미소장
19 Binding of IFITM1 enhances the inhibiting effect of caveolin-1 on ERK activation 네이버 미소장