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An oligonucleotide microarray system was developed for the simultaneous detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, porcine enteric calicivirus, porcine group A and C rotavirus. RNAs of the reference viruses and porcine diarrhea samples were extracted and amplified using onestep multiplex RTPCR in the presence of cyanine 5dCTP and hybridized on the microarray chip that spotted the virusspecific oligonucleotides. This system were approximately 10 to 100fold higher in sensitivity than conventional RTPCR, and the assay time was less than 3 hours. The relative sensitivity and specificity were 92% and 72.2%, respectively, based on 102 porcine diarrhea samples using RTPCR as gold standard. These results suggested that the oligonucleotide microarray system in this study be probably more reliable and reproducible means for detecting porcine enteric viruses and that it could be of substantial use in routine diagnostic laboratories.

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기사명 저자명 페이지 원문 목차
Development of oligonucleotide microarray system for differential diagnosis of enteric viruses in diarrheic fecal samples in pigs 박남용, 김용환, 조호성 pp.489-496

Immune response and efficacy of pigeon pox virus vaccine and fowl pox virus vaccine in chickens Niraj Kanti Shil, Md Siddique Rahman, MT Hossain, MT Islam, MM Rahman, KBM Saiful Islam, Se-Yeoun Cha, Hyung-Kwan Jang, Hee-Jong Song pp.497-503

Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip 박남용, 나성호, 조호성 pp.505-510

Monocrotophos poisoning in wild mallards(Anas platyrhynchos) 심항섭, 우종태, 송은아, 김혜성, 김인섭, 정해선, 박준조 pp.545-550

Application of the melanocortin 1 receptor(MC1R) gene for discrimination of Hanwoo from Holstein beef using real-time polymerase chain reaction(PCR) 나도경, 박은정, 이성모, 이정구 pp.557-562

Histopathologic and electron microscopic findings of canine malignant melanoma from the lower limb and digit 우계형, 김하영, 김진영, 박정원, 백은정, 최정업 pp.533-538

Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo 신종봉, 김용환, 박남용, 조호성 pp.527-532

Bovine leukosis in a slaughtered cow 이상명, 최해연, 한성태, 윤호규, 곽학구, 정찬우, 박선준, 장래훈 pp.551-555

Canine hemangiopericytoma in a Golden Retriever : A case report 김하영, 우계형, 최정업, 황범태, 박정원 pp.539-544

Seroprevalence of brucellosis in small ruminants in selected area of Bangladash[i.e. Bangladesh] Mohammad Jasim Uddin, Md Siddiqur Rahman, Sayeda Hasina Akter, Mohammad Arif Hossain, Md Taohidul Islam, Md Ariful Islam, Jin-Ho Park, Hee-Jong Song pp.511-525

참고문헌 (12건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Altman DG, (1999) Practical statistics for medical research, Chapman & Hall/CRC Press, Boca Raton/London/New York/Washington, DC 미소장
2 Call DR, (2001) Detecting and genotyping Esche?richia coli O157:H7using multiplexed PCR and nucleic acid microarrays, 미소장
3 Chizhikov V, (2001) Microarray analysis of microbial virulence factors, 미소장
4 Chizhikov V, (2002) Detection and genotyping of human group A rotaviruses by oligo?nucleotide microarray hybridization, 미소장
5 Elschner M, (2002) Nested reverse transcriptase?polymerase chain reaction for the detec?tion of group A rotaviruses, 미소장
6 Kwok S, (1989) Avoiding false positives with PCR, 미소장
7 Murphy FA, (1999) Laboratory diagnosis of viral diseases, Academic press, Calif 미소장
8 Neverov AA, (2006) Genotyping of measles virus in clinical specimens on the basis of oligonucleotide microarray hybridization patterns, 미소장
9 Rozen S, (2000) Primer3 on the WWW for general users and for biologist programmers, 미소장
10 Bohl EH, (1980) and 23?nm virus?like particles asso? ciated with diarrhea in young pigs, 미소장
11 Straw BE, (2006) Differential diagnosis of swine diseases, Iowa State University Press, Ames, IA 미소장
12 Wang RF, (2002) Design and evaluation of oligonucleotide?microarray method for the detection of human intestinal bac?teria in fecal samples, 미소장

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