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Wild rice, Oryza grandiglumis shows hyper-resistance response to pathogen infection. In order to identify genes necessary for defense response in plants, we have carried out a subtractive hybridization coupled with a cDNA macroarray. An acidic PATHOGENESIS-RELATED1(PR1) gene of the wild rice is highly identical to the acidic PR1 genes of different plant species. The OgPR1a cDNA has an apparent single open reading frame with a predicted molecular mass 40,621 Da and an isoelectic point of 5.14. Both in silico analysis and a transient expression assay in onion epidermal cells revealed that the OgPR1a protein could be localized in intercellular space in plants. The OgPR1a mRNA was strongly transcribed by the exogenous treatment with ethylene and jasmonic acid as well as protein phosphataseinhibitors. Additionally, ectopic expression of the OgPR1a conferred disease resistance on Arabidopsis to the bacterial and fungal infections.

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참고문헌 (39건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Agrawal, G. K., Jwa, N. S. and Rakwal, R. 2000. A novel rice (Oryza sativa L.) acidic PR1 gene highly responsive to cut, phytohormones, and protein phosphatase inhibitors. Biochem. Biophys. Res. Commun. 274:157-165. 미소장
2 Increased Tolerance to Two Oomycete Pathogens in Transgenic Tobacco Expressing Pathogenesis-Related Protein 1a 네이버 미소장
3 Transcriptional responses to cantharidin, a protein phosphatase inhibitor, in Arabidopsis thaliana reveal the involvement of multiple signal transduction pathways. 네이버 미소장
4 Brederode, F. T., Linthorst, H. J. M. and Bol. J. F. 1991. Differential induction of acquired resistance and PR gene expression in tobacco by virus infection, ethephon treatment, UV light and wounding. Plant Mol. Biol. 17:1117-1125. 미소장
5 Buchel, A. S. and Linthorst, H. J. M. 1999. PR1: a group of plant proteins induced upon pathogen infection. In: Pathogenesisrelated proteins in plants, eds. By S. K. Datta and S. Muthukrishnan, pp. 21-47. CRC Press, United States. 미소장
6 Synthesis and localization of pathogenesis-related proteins in tobacco. 네이버 미소장
7 Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. 네이버 미소장
8 Protein dephosphorylation mediates salicylic acid‐induced expression of PR‐1 genes in tobacco 네이버 미소장
9 Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group. 네이버 미소장
10 David, S. J. and Vierstra, R. D. 1996. Soluble derivatives of green fluorescent protein (GFP) for use in Arabidopsis thaliana. Weeds World 3:43-48. 미소장
11 De Vos, M., Van Oosten, V. R., Van Poecke, R. M., Van Pelt, J. A., Pozo, M. J., Mueller, M. J., Buchala, A. J., Metraux, J. P., Van Loon, L. C., Dicke, M. and Pieterse, C. M. 2005. Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol. Plant-Microbe Interact. 18:923-937. 미소장
12 Denancé, N., Sánchez-Vallet, A., Goffner, D. and Molina, A. 2013. Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front. Plant Sci. 4:155. 미소장
13 The Role of Ethylene and Wound Signaling in Resistance of Tomato to Botrytis cinerea 네이버 미소장
14 Han, S. W. and Jung, H. W. 2013. Molecular sensors for plant immunity; pattern recognition receptors and race-specific resistance proteins. J. Plant Biol. 56:357-366. 미소장
15 Ectopic expression of ubiquitin-conjugating enzyme gene from wild rice, OgUBC1, confers resistance against UV-B radiation and Botrytis infection in Arabidopsis thaliana 네이버 미소장
16 The plant immune system 네이버 미소장
17 Jung, H. W. and Hwang, B. K. 2000. Isolation, partial sequencing, and expression of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria. Mol. Plant-Microbe Interact. 13:136-142. 미소장
18 Distinct roles of the pepper hypersensitive induced reaction protein gene CaHIR1 in disease and osmotic stress, as determined by comparative transcriptome and proteome analyses 네이버 미소장
19 Priming in Systemic Plant Immunity 네이버 미소장
20 Katagiri, F. and Tsuda, K. 2010. Understanding the plant immune system. Mol. Plant-Microbe Interact. 23:1531-1536. 미소장
21 Simultaneous RNA-seq analysis of a mixed transcriptome of rice and blast fungus interaction. 네이버 미소장
22 Analysis of differentially expressed transcripts of fungal elicitor- and wound-treated wild rice (Oryza grandiglumis). 네이버 미소장
23 Pepper gene encoding a basic pathogenesis-related 1 protein is pathogen and ethylene inducible 네이버 미소장
24 Distinct Roles for Jasmonate Synthesis and Action in the Systemic Wound Response of Tomato 네이버 미소장
25 Constitutive Expression of Pathogenesis-Related Proteins PR-1, GRP, and PR-S in Tobacco Has No Effect on Virus Infection 네이버 미소장
26 Characteristic expression of twelve rice PR1 family genes in response to pathogen infection, wounding, and defense-related signal compounds (121/180). 네이버 미소장
27 Plant Physiology: Three Years into a New Era 네이버 미소장
28 Detection of pathogenesis-related proteins (PR or b ) and of other proteins in the intercellular fluid of hypersensitive plants infected with tobacco mosaic virus 네이버 미소장
29 Payne, G., Middlesteadt, W., Desai, N., Williams, S., Dincher, S., Carnes, J. and Ryals, J. 1989. Isolation and sequence of a genomic clone encoding the basic form of pathogenesis-related protein 1 from Nicotiana tabacum. Plant Mol. Biol. 12:595-596. 미소장
30 Reymond, P. and Farmer, E. E. 1998. Jasmonate and salicylate as global signals for defense gene expression. Curr. Opin. Plant Biol. 1:404-411. 미소장
31 Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses. 네이버 미소장
32 Sessa, G., Yang, X. Q., Raz, V., Eyal, Y. and Fluhr, R. 1995. Darkinduction and subcellular localization of the pathogenesisrelated PRB-1b protein. Plant Mol. Biol. 28:537-547. 미소장
33 Shin, S. H., Pak, J. H., Kim, M. J., Kim, H. J., Lee, J. H., Kim, D. H., Choi, H. K., Kang, K. H., Jeong, J. U., Kang, C. S., Jung H. W. and Chung, Y. S. 2012. Cloning and characterization of pathogenesis-related gene 10a (OgPR10a) derived from wild rice (Oryza grandiglumis). Kor. J. Breed. Sci. 44:1-10. 미소장
34 Spoel, S. H. and Dong, X. 2012. How do plants achieve immunity? Defense without specialized immune cells. Nat. Rev. Immun. 12:89-100. 미소장
35 Takeuchi, Y., Dotson, M. and Keen, N. T. 1992. Plant transformation:a simple particle bombardment device based on flowing helium. Plant Mol. Biol. 18:835-839. 미소장
36 Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’ 네이버 미소장
37 Plant Pathology and Plant Pathogens 네이버 미소장
38 Significance of inducible defense-related proteins in infected plants. 네이버 미소장
39 Zhang, J., Du, X., Wang, Q., Chen, X., Lv, D., Xu, K., Qu, S. and Zhang, Z. 2010. Expression of pathogenesis related genes in response to salicylic acid, methyl jasmonate and 1aminocyclopropane-1carboxylic acid in Malus hupehensis (Pamp.)Rehd. BMC Res. Notes 3:208. 미소장