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초록보기

GA733-2, an epithelial cell adhesion molecule highly expressed at the human colorectal carcinoma cell surface, is a candidate protein for the development of a colorectal cancer preventive vaccine. In this study, a signal peptide and c-terminal transmembrane domain truncated GA733-2 was fused to a His-tag, and the recombinant protein (rGA733-2) was expressed in Escherichia coli Rosetta (DE3) pLysS. rGA733-2 was expressed in the form of inclusion bodies, and the protein was purified using either a serial dialyzed urea refolding method or metal ion exchange column after 8 M urea solubilization. The efficiency of rGA733-2 purification was increased three times using the urea refolding method compared to the column purification. A polyclonal antibody against the rGA733-2 was generated in rabbit using the refolded rGA733-2 as an antigen. The polyclonal antibodies (pAb rGA733-2) sensitively detected rGA733-2, which was heterologously expressed in bacteria and plants, and also detected native GA733-2 proteins in mouse and human cancer cells. pAb rGA733-2 showed high specificity against tobacco-expressed rGA733-2 in an immunoprecipitation assay. rGA733-2 and pAb rGA733-2 could serve as useful tools toward the development of a plant-derived colorectal cancer vaccine.

권호기사

권호기사 목록 테이블로 기사명, 저자명, 페이지, 원문, 기사목차 순으로 되어있습니다.
기사명 저자명 페이지 원문 목차
Ultrastructural observations on spermiogenesis in the peanut worm, Phascolosoma esculenta(Sipuncula: Phascolosomatidea) Lingli Long, Zhang Sheng, Junquan Zhu pp.221-229
Description of a seminatural habitat of the endangered Suweon treefrog Hyla suweonensis Amaël Borzée, 장이권 pp.216-220
Selection of air pollution tolerant plants through the 20-years-long transplanting experiment in the Yeocheon industrial area, southern Korea 김경순, 피정훈, 안지홍, 임치홍, 이창석 pp.208-215
Anti-apoptotic effects of silk fibroin hydrolysate in RIN5F cell on high glucose condition 박준홍, 조경인, Hajin Nam, 최농훈, 서준교 pp.201-207
Fermented bitter gourd extract differentially regulates lipopolysaccharide-induced cytokine gene expression through nuclear factor-κB and interferon regulatory factor-1 장석원, 임수근, 이동섭, 석경호, 이원하 pp.194-200
Purification of human carcinoma antigen GA733-2 expressed in Escherichia coli and production of its polyclonal antibody in rabbit 박세희, 김아영, 마상훈, 김현민, 강형식, 맹진수, 고기성, 정인식, 정영희 pp.188-193
Comparisons of behavior and synaptic plasticity among three C57BL/6 substrains 강민경, 류현희, 이용석 pp.181-187
Optimization of Matrigel-based culture for expansion of neural stem cells 이승원, 이혜정, 황한성, 고기성, 한동욱, 고기남 pp.175-180
Embryoid bodies formation from chicken primordial germ cells Hui Xiong, Yabin Pu, Qingyun Hu, Zhiqiang Shan, Pengfei Hu, Weijun Guan, Yuehui Ma pp.168-174
Fractal analysis of cell boundary ultrastructure imaged by atomic force microscopy 김유진, 김희대, 김현희, 신상모, Chi Jung Kang, 이건호 pp.161-167

참고문헌 (13건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
1 EpCAM: A new therapeutic target for an old cancer antigen. 네이버 미소장
2 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding 네이버 미소장
3 Replication of tobacco mosaic virus 네이버 미소장
4 Plant-derived EpCAM antigen induces protective anti-cancer response. 네이버 미소장
5 Leaf proteome analysis of transgenic plants expressing antiviral antibodies. 네이버 미소장
6 Fox JL. 2006. Turning plants into protein factories. Nat Biotechnol. 24:1191–1193. 미소장
7 Gleba Y, Klimyuk V, Marillonnet S. 2005. Magnifection—a new platform for expressing recombinant vaccines in plants. Vaccine. 23:2042–2048. 미소장
8 Expression of the GA733 gene family and its relationship to prognosis in pulmonary adenocarcinoma 네이버 미소장
9 EpCAM expression in retinoblastoma: a novel molecular target for therapy. 네이버 미소장
10 Momburg F, Moldenhauer G, Hammerling GJ, Moller P. 1987. Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Res. 47:2883–2891. 미소장
11 Rybicki EP. 2008. Plant-made vaccines for humans and animals. Plant Biotechnol J. 8:620–637. 미소장
12 Plant Proteases: From Phenotypes to Molecular Mechanisms 네이버 미소장
13 Immunization with a plant-produced colorectal cancer antigen. 네이버 미소장

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