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결과 내 검색 동의어 포함

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초록보기

본 연구는 Peucedanum insolens Kitagawa 뿌리의 여러 유기용매 분획물에 대한 항산화 활성과 LPS 처리된 RAW264.7 세포에서 항염증 효과를 조사하기 위해 수행되었다. 이 연구를 위해 P. insolens Kitagawa 뿌리를 먼저 실온에서 1주일 동안 건조하여 잘게 썰고 70% 에탄올로 추출한 다음 hexane, chloroform, ethyl acetate 및 물로 연속적으로 분획하였다. DPPH (1,1-diphenyl-2-picrylhydrazyl) 라디칼 소거능 분석과 total polyphenol 및 total flavonoid 함량을 측정하여 유기용매 분획물의 항산화 활성을 평가했다. RAW264.7 세포에서 iNOS, COX-2, IL-1β 및 IL-6과 같은 염증 매개 유전자의 발현 억제 수준을 측정하여 분획의 항 염증 효능을 평가했다. 본 연구 결과는 P. insolens Kitagawa 뿌리의 ethyl acetate 분획물이 상대적으로 높은 함량의 total flavonoid (34.08±1.68 μg QE/mg)와 total polyphenol (154.1±3.2 μg의 GE/mg)을 함유하고 있음을 분명히 보여주었다. DPPH 분석 결과 P. insolens Kitagawa는 ethyl acetate 분획에서 강력한 자유 라디칼 소거 활성을 가지고 있음을 보여주었다. Ethyl acetate 분획 및 hexane 분획은 모두 RAW264.7 세포에서 24시간 동안 LPS (1 μg/ml) 처리에 의해 유도된 산화 질소 생성에 대해 강력한 억제력을 나타냈다. 본 연구결과는 또한 P. insolens Kitagawa의 hexane 분획 및 ethyl acetate 분획 모두 RAW264.7 세포에서 24시간 동안 LPS 처리에 의해 과발현된 iNOS, IL-1β 및 IL-6의 mRNA 수준을 강력하게 억제하는 것으로 나타났다. 이러한 결과는 P. insolens Kitagawa가 항 염증 활성에 강한 효능을 가진 화합물을 포함할 수 있음을 시사한다. 염증 조절에 대한 P. insolens Kitagawa 분획물의 더 자세한 효과를 이해하기 위하여 이 분획물이 cytokine 신호 전달 경로 및 염증 신호 경로에 대한 조절작용과 같은 추가적인 작용기전 연구가 필요하다.

This study was performed to investigate the antioxidant activities of subfractions of Peucedanum insolens Kitagawa root in various organic solvents and their anti-inflammatory effects on LPS-treated RAW264.7 cells. First, P. insolens Kitagawa roots were dried at room temperature for one week, chopped, and extracted with 70% ethanol. The resulting extracts were successively sub-fractionated with hexane, chloroform, ethyl acetate, and water. The antioxidant potential of the fractions was evaluated using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay and by measuring total polyphenol and flavonoid contents. The anti-inflammatory potency of the fractions was evaluated by measuring the inhibition levels of the expressions of inflammatory-mediated genes and proteins (e.g., iNOS, COX-2, IL-1β, and IL-6) in RAW264.7 cells. The results clearly showed that the ethyl acetate fraction of the P. insolens Kitagawa root contained relatively high total flavonoid (34.08±1.68 μg of quercetin equivalents per mg) and total polyphenol (154.1±3.2 μg of gallic acid equivalents per mg) contents. The DPPH assay results showed that the P. insolens Kitagawa root possessed strong free radical scavenging activity in the ethyl acetate fraction. Both the ethyl acetate and hexane fractions showed strong inhibitory potencies to nitric oxide production induced by lipopolysaccharide (1 μg/ ml) treatment for 24 hr in RAW264.7 cells. The results also showed that both the hexane and ethyl acetate fractions of the P. insolens Kitagawa root strongly inhibited mRNA levels of iNOS, IL-1β, and IL-6, which were overexpressed by LPS treatment for 24 hr in the RAW264.7 cells. These results suggest that P. insolens Kitagawa root may contain compounds that possess strong potency for anti-inflammatory activity. Further studies are needed to discover more detailed modes of action of P. insolens Kitagawa root fractions against inflammation modulation, such as the regulation of cytokine signaling and inflammatory signaling pathways.

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기사명 저자명 페이지 원문 목차
Antioxidant activities of Peucedanum insolens Kitagawa root extracts and their anti-inflammatory effects on LPS-treated RAW264.7 cells = 왕산방풍의 뿌리로부터 제조한 유기용매 분획물에서의 항산화 활성 및 RAW264.7 세포주에서의 항염증 효능 Jin-Ik Kim, Yong-Won Choi, Geun-June Choi, Ji-An Kang, In-Young Lee, Nandintsetseg Narantuya, Myong-Seok Oh, Sik-Jae Cho, Ja-Young Moon p. 17-27

Comparative analysis of detection methods for food-borne pathogens in fresh-cut agricultural materials = 신선 농산물내 식중독균 검출 방법의 비교 분석 Hye-Jeong Jang, Hye-Jeong Kim, Ji-in Park, Sun-Nyoung Yu, Bo-Bae Park, Gang-Ja Ha, Soon-Cheol Ahn, Dong-Seob Kim p. 10-16

Fucoidan stimulates glucose uptake via the PI3K/AMPK pathway and increases insulin sensitivity in 3T3-L1 adipocytes = 후코이단의 3T3-L1 지방세포에서 PI3K/AMPK 경로를 통한 포도당 흡수 촉진 및 인슐린 민감성 증진 효과 Ji Hee Lee, Jae Eun Park, Ji Sook Ha p. 1-9

OmpR is essential for growth and expression of virulence-related genes in the fish pathogen Edwardsiella piscicida = 어류 병원체 Edwardsiella piscicida의 OmpR은 생육과 병원성과 관련된 유전자의 발현에 필수적 Durga Ray, Yeon Ha Kim, Yunjeong Choe, Ho Young Kang p. 28-36

국내에서 분리된 Pseudomonas syringae pv. actinidiae biovar 3 균주들의 subgroup 분포 = Distribution of subgroups in Pseudomonas syringae pv. actinidiae biovar 3 strains isolated from Korea 이영선, 김경희, 정재성 p. 52-58

Thrichoderma atroviride 배양액의 tyrosinase 억제제에 관한 연구 = Inhibition of tyrosinase by metabolites originating from Thrichoderma atroviride 강동우, 김규민, 김예성, 서유진, 송다영, 오다윤, 최시온, 황주현, 김삼웅, 방규호, 갈상완 p. 47-51

생마늘 및 흑마늘 열수추출 분말이 고콜레스테롤 혈증 흰쥐의 체내 지질조성에 미치는 영향 = Effects of fresh and black garlic hot water extract powder on the lipid composition of hypercholesterolemia rats 강민정, 신정연, 이수정, 신정혜 p. 37-46

바이오필름을 생성하는 병원성 구강 세균을 제어하는 새로운 접근법 = New approaches to the control of pathogenic oral bacteria 조수정 p. 100-108

Pseudomonas sp. EML8 균주를 이용한 폐식용류로부터 medium-chain-length poly(3-hydroxyalkanoates) 생합성 = Production of medium-chain-length poly(3-hydroxyalkanoates) by Pseudomonas sp. EML8 from waste frying oil 김태경, 김종식, 정정욱 p. 90-99

개불 라이소자임 유래 항균성 모델 펩타이드(Uu-ilys-CF)의 재조합 단백질 생산 및 항균 활성 = Recombinant production and antimicrobial activity of an antimicrobial model peptide (Uu-ilys-CF) derived from spoon worm lysozyme, Uu-ilys 오혜영, 고혜진, 박남규 p. 83-89

제주 전통 발효식품 쉰다리에서 분리한 세균의 군집 조사 및 어류질병세균과 인체유해세균에 대한 항균활성효과 = Phylogenetic diversity and antibacterial activity of bacteria from Shindari of Jeju traditional fermented food 유영수, 허문수 p. 73-82

COI 기반 제한효소 절편 길이 다형성(RFLP)을 이용한 새우젓 분석 = Identification of salted opossum shrimp using COI-based restriction fragment length polymorphism 박주현, 문수영, 강지혜, 정명화, 김상조, 최희정 p. 66-72

매실 부탄올 분획물에 의한 혈관 신생 억제 효과 = Angiogenesis-inhibiting effects of Prunus mume butanol fractions on human umbilical vein endothelial cells 민혜지, 김정호, 허지안, 원영선, 서권일 p. 59-65

참고문헌 (46건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Agrawal, S., Kulkarni, G. T. and Sharma, V. N. 2011. A comparative study on the antioxidant activity of methanolic extracts of Terminalia paniculata and Madhuca longifolia. Free Radic. Antiox. 1, 62-68. 미소장
2 Bendary, E., Francis, R. R., Ali, H. M. G., Sarwat, M. I. and El Hady, S. 2013. Antioxidant and structure-activity relationships (SARs) of some phenolic and anilines compounds. Ann. Agric. Sci. 58, 173-181. 미소장
3 Bertolini, A., Ottani, A. and Sandrini, M. 2002. Selective COX-2 inhibitors and dual acting anti-inflammatory drugs:critical remarks. Curr. Med. Chem. 9, 1033-1043. 미소장
4 Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT - Food Sci. Technol. 28, 25-30. 미소장
5 Brüne, B., Dehne, N., Grossmann, N., Jung, M., Namgaladze, D., Schmid, T., Von Knethen, A. and Weigert, A. 2013. Redox control of inflammation in macrophages. Antioxid. Redox Signal 19, 595-637. 미소장
6 Chi, H. J. and Han, D. S. 1976. New or Noteworthy Medicinal Plants from Korea (II). Kor. J. Pharmacog. 7, 69-71. 미소장
7 Chi, H. J. and Kim, H. S. 1981. Coumarins from the Root of Peucedanum insolens Kitagawa. Kor. J. Pharmacog. 12, 79-81. 미소장
8 Chi, H. J. and Kim, H. S. 1982. Pharmacognostical Studies on the Root of Peucedanum insolens Kitagawa (II). Kor. J. Pharmacog. 13, 48-48 미소장
9 Choi, Y. H., Kim, G. Y. and Lee, H. H. 2014. Anti-inflammatory effects of cordycepin in lipopolysaccharide-stimulated RAW264.7 macrophages through toll-like receptor 4-mediated suppression of mitogen-activated protein kinases and NF-κB signaling pathways. Drug Des. Devel. Ther. 8, 1941-1953. 미소장
10 Côté, J., Caillet, S., Doyon, G., Sylvain, J. F. and Lacroix, M. 2010. Bioactive compounds in cranberries and their biological properties. Crit. Rev. Food Sci. Nutr. 50, 666-679. 미소장
11 Davis, D. W. 1947. Determination of flavonones in citrus juice. Anal. Chem. 19, 46-48. 미소장
12 Du, Z., Liu, H., Zhang, Z. and Li, P. 2013 Antioxidant and anti-inflammatory activities of Radix Isatidis polysaccharide in murine alveolar macrophages. Int. J. Biol. Macromol. 58, 329-335. 미소장
13 Goraca, A., Huk-Kolega, H., Kleniewska, P., Piechota-Polanczyk, A. and Skibska, B. 2013. Effects of lipoic acid on spleen oxidative stress after LPS administration. Pharmacol. Rep. 65, 179-186. 미소장
14 Han, J. M., Lee, E. K., Gong, S. Y., Sohng, J. K., Kang, Y. J. and Jung, H. J. 2019. Sparassis crispa exerts antiinflammatory activity via suppression of TLR-mediated NFκB and MAPK signaling pathways in LPS-induced RAW264.7 macrophage cells. J. Ethnopharmacol. 231, 1018. 미소장
15 Han, S. Y., Yi, Y. S., Jeong, S. G., Hong, Y. H., Choi, K. J., Hossain, M. A., Hwang, H., Rho, H. S., Lee, J., Kim, J. H. and Cho, J. Y. 2018. Ethanol extract of Lilium bulbs plays an antiinflammatory role by targeting the IKK-mediated NF-κB pathway in macrophages. Am. J. Chin. Med. 46, 12811296. 미소장
16 Heo, S. J., Jang, J., Ye, B. R., Kim, M. S., Yoon, W. J., Oh, C., Kang, D. H., Lee, J. H., Kang, M. C. and Jeon, Y. J. 2014. Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW264.7 cells. Food Chem. Toxicol. 67, 169-175. 미소장
17 Hewett, J. A. and Roth, R. A. 1993. Hepatic and extrahepatic pahtobiology of bacterial lipopolysaccharides. Pharmacol. Rev. 45, 382-411. 미소장
18 Hwang, Y. J., Lee, E. J., Kim, H. R. and Hwang, K. A. 2013. In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var lilacina. BMC Complement. Altern. Med. 13, 310. 미소장
19 Khan, S., Choi, R. J., Shehzad, O., Kim, H. P., Islam, M. N., Choi, J. S. and Kim, Y. S. 2013. Molecular mechanism of capillarisin-mediated inhibition of MyD88/TIRAP inflammatory signaling in in vitro and in vivo experimental models. J. Ethnopharmacol. 145, 626-637. 미소장
20 Khan, S., Shin, E. M., Choi, R. J., Jung, Y. H., Kim, J. W., Tosun, A. and Kim, Y. S. 2011. Suppression of LPS-induced inflammatory and NF-κB responses by anomalin in RAW 264.7 macrophages. J. Cell Biochem. 112, 2179-2188. 미소장
21 Kim, Y. H., Lee, S. H., Lee, J. Y., Choi, S. W., Park, J. W. and Kwon, T. K. 2004. Triptolide inhibits murine-inducible nitric oxide synthase expression by down-regulating lipopolysaccharide-induced activity of nuclear factor-κB and c-Jun NH 2-terminal kinase. Eur. J. Pharmacol. 494, 1-9. 미소장
22 Kroncke, K. D., Fehsel, K. and Kolb-Bachofen, V. 1998. Inducible nitric oxide synthase in human diseases. Clin. Exp. Immunol. 113, 147-156. 미소장
23 Losada-Barreiro, S. and Bravo-Díaz, C. 2017. Free radicals and polyphenols: The redox chemistry of neurodegenerative diseases. Eur. J. Med. Chem. 133, 379-402. 미소장
24 Madamanchi, N. R., Vendrov, A. and Runge, M. S. 2005. Oxidative stress and vascular disease. Arter. Thromb. Vasc. Biol. 25, 29-38. 미소장
25 Maione, F., Russo, R., Khan, H. and Mascolo, N. 2016. Medicinal plants with anti-inflammatory activities. Nat. Prod. Res. 30, 13431352. 미소장
26 Mercurio, F. and Manning, A. M. 1999. Multiple signals converging on NF-κB. Curr. Opin. Cell Biol. 11, 226-232. 미소장
27 Mills, E. L. and O'Neill, L. A. 2016. Reprogramming mitochondrial metabolism in macrophages as an anti-inflammatory signal. Eur. J. Immunol. 46, 13-21. 미소장
28 Mordan, L. J., Burnett, T. S., Zhang, L. X., Tom, J. and Cooney, R. V. 1993. Inhibitors of endogenous nitrogen oxide formation block the promotion of neoplastic transformation in C3H 10 T1/2 fibroblasts. Carcinogenesis 14, 1555-1559. 미소장
29 Nam, J. Y. and Ryu, K. S. 1975. Pharmacognostical Studies on Korean ‘Bang Poong’. Kor. J. Pharmacog. 6, 151-159 미소장
30 Niu, X., Xing, W., Li, W., Fan, T., Hu, H. and Li, Y. 2012. Isofraxidin exhibited anti-inflammatory effects in vivo and inhibited TNF-α production in LPS-induced mouse peritoneal macrophages in vitro via the MAPK pathway. Int. Immunopharmacol. 14, 164-171. 미소장
31 Oberoi, H. S. and Sandhu, S. K. 2015. Therapeutic and nutraceutical potential of bioactive compounds extracted from fruit residues AU—Babbar, Neha. Crit. Rev. Food Sci. Nutr. 55, 319-337. 미소장
32 Ohshima, H. and Bartsch, H. 1994. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat. Res. 305, 253-264. 미소장
33 Pan, S. Y., Zhou, S. F., Gao, S. H., Yu, Z. L., Zhang, S. F., Tang, M. K., Sun, J. N., Ma, D. L., Han, Y. F., Fong, W. F. and Ko, K. M. 2013. New perspectives on how to discover drugs from herbal medicines: CAM's outstanding contribution to modern therapeutics. Evid. Based Complement. Alternat. Med. 2013, 627375-627401. 미소장
34 Perkins, N. D. 2000. The REL/NF-κB family: Friend and foe. Trends Biochem. Sci. 25, 434-440. 미소장
35 Rahman, K. Studies on free radicals, antioxidants, and cofactors. 2007. Clin. Interv. Aging 2, 219-236. 미소장
36 Rajapakse, N., Kim, M. M., Mendis, E. and Kim, S. K. 2008. Inhibition of inducible nitric oxide synthase and cyclooxy genase-2 in lipopolysaccharide-stimulated RAW264. 7 cells by carboxybutyrylated glucosamine takes place via downregulation of mitogen-activated protein kinase-mediated nuclear factor-κB signaling. Immunology 123, 348-357. 미소장
37 Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic. 16, 144-158. 미소장
38 Soobrattee, M. A., Neergheen, V. S., Luximon-Ramma, A., Aruoma, O. I. and Bahorun, T. 2005. Phenolics as potential antioxidant therapeutic agents: Mechanism and actions. Mutat. Res. 579, 200-213. 미소장
39 Takamata, A. 1992. Effect of vagotomy on cardiovascular adjustment to hyperthermia in rats. Jpn. J. Physiol. 42, 641-652. 미소장
40 Tapiero, H., Tew, K.D., Ba, N. and Mathe, G. 2002. Polyphenols:do they play a role in the prevention of human pathologies? Biomed. Pharmacother. 56, 200-207. 미소장
41 Tungmunnithum, D., Thongboonyou, A., Pholboon, A. and Yangsabai, A. 2018. Flavonoids and other phenolic com pounds from medicinal plants for pharmaceutical and med ical aspects: An overview. Medicines 5, 93. 미소장
42 Wadsworth, T. L. and Koop, D. R. 1999. Effects of wine polyphenolics quercetin and resveratrol on pro-inflammatory cytokine expression in RAW 264.7 macrophages. Biochem. Pharmacol. 57, 941-949. 미소장
43 Wang, X. and Lau, H. 2006. Prostaglandin E2 potentiates the immunologically stimulated histamine release from human peripheral blood-derived mast cells through EP1/EP3receptors. Allergy 61, 503-506. 미소장
44 Yang, C. S., Landau, J. M., Huang, M. T. and Newmark, H. L. 2001. Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu. Rev. Nutr. 21, 381-406. 미소장
45 Yeom, M., Park, J., Lim, C., Sur, B., Lee, B., Han, J. J., Choi, H. D., Lee, H. and Hahm, D. H. 2015. Glucosylceramide attenuates the inflammatory mediator expression in lipopolysaccharide-stimulated RAW264. 7 cells. Nutr. Res. 35, 241-250. 미소장
46 Yoshino, S., Sasatomi, E. and Ohsawa, M. 2000. Bacterial lipopolysaccharide acts as an adjuvant to induce autoimmune arthritis in mice. Immunobiology 99, 607-614. 미소장

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