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Proteomic identification of arginine-methylated proteins in colon cancer cells and comparison of messenger RNA expression between colorectal cancer and adjacent normal tissues / Yongchul Lim ; Da Young Gang ; Woo Yong Lee ; Seong Hyeon Yun ; Yong Beom Cho ; Jung Wook Huh ; Yoon Ah Park ; Hee Cheol Kim 1

[요약] 1

INTRODUCTION 1

METHODS 2

RESULTS 4

DISCUSSION 7

SUPPLEMENTARY MATERIALS 8

REFERENCES 8

초록보기

Purpose: Identification of type I protein arginine methyltransferase (PRMT) substrates and their functional significance during tumorigenesis is becoming more important. The present study aimed to identify target substrates for type I PRMT using 2-dimensional (2D) gel electrophoresis (GE) and 2D Western blotting (WB).Methods: Using immunoblot analysis, we compared the expression of type I PRMTs and endogenous levels of arginine methylation between the primary colorectal cancer (CRC) and adjacent noncancerous tissues paired from the same patient. To identify arginine-methylated proteins in HCT116 cells, we carried out 2D-GE and 2D-WB with a type I PRMT product-specific antibody (anti-dimethyl-arginine antibody, asymmetric [ASYM24]). Arginine-methylated protein spots were identified by mass spectrometry, and messenger RNA (mRNA) levels corresponding to the identified proteins were analyzed using National Center for Biotechnology Information (NCBI) microarray datasets between the primary CRC and noncancerous tissues.Results: Type I PRMTs and methylarginine-containing proteins were highly maintained in CRC tissues compared to noncancerous tissues. We matched 142 spots using spot analysis software between a Coomassie blue (CBB)-stained 2D gel and 2D-WB, and we successfully identified 7 proteins that reacted with the ASYM24 antibody: CACYBP, GLOD4, MAPRE1, CCT7, TKT, CK8, and HSPA8. Among these proteins, the levels of 4 mRNAs including MAPRE1, CCT7, TKT, and HSPA8 in CRC tissues showed a statistically significant increase compared to noncancerous tissues from patients using the NCBI microarray datasets.Conclusion: Our results indicate that the method shown here is useful in identifying arginine-methylated proteins, and significance of arginine modification in the proteins identified here should be further identified during CRC development.

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(Editorial) Future direction of enhanced recovery after surgery (ERAS) program in colorectal surgery In Ja Park p. 1-2

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Extended lymphadenectomy in locally advanced rectal cancers : a systematic review Balaji Mahendran, Supriya Balasubramanya, Simone Sebastiani, Sebastian Smolarek p. 3-12

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Long-term evolution of continence and quality of life after sphincteroplasty for obstetric fecal incontinence Vicente Pla-Martí, Jose Martín-Arévalo, Rosa Martí-Fernández, David Moro-Valdezate, Stephanie García-Botello, Alejandro Espí-Macías, Miguel Mínguez-Pérez, Maria Dolores Ruiz-Carmona, Jose Vicente Roig-Vila p. 13-19

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(The) "reverse air leak test" : a new technique for the assessment of low colorectal anastomosis Francesco Crafa, Augusto Striano, Francesco Esposito, Amalia Rosaria Rita Rossetti, Mario Baiamonte, Valeria Gianfreda, Antonio Longo p. 20-27

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Oncological and functional outcomes of transanal total mesorectal excision in a teaching hospital in the Netherlands Joost A.G. van der Heijden, Kelly G.H. van de Pas, Frank J.C. van den Broek, Francois M.H. van Dielen, Gerrit D. Slooter, Adriana J.G. Maaskant-Braat p. 28-35

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(The) impact of variations in care and complications within a colorectal enhanced recovery after surgery program on length of stay James Wei Tatt Toh, Jack Cecire, Kerry Hitos, Karen Shedden, Fiona Gavegan, Nimalan Pathmanathan, Toufic El Khoury, Angelina Di Re, Annelise Cocco, Alex Limmer, Tom Liang, Kar Yin Fok, James Rogers, Edgardo Solis, Grahame Ctercteko p. 36-46

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Clinical validation of implementing enhanced recovery after surgery protocol in elderly colorectal cancer patients Wooree Koh, Chul Seung Lee, Jung Hoon Bae, Abdullah Al-Sawat, In Kyu Lee, Hyeong Yong Jin p. 47-52

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Predictors of postoperative urinary retention after semiclosed hemorrhoidectomy Hong Yoon Jeong, Seok Gyu Song, Jong Kyun Lee p. 53-59

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Proteomic identification of arginine-methylated proteins in colon cancer cells and comparison of messenger RNA expression between colorectal cancer and adjacent normal tissues Yongchul Lim, Da Young Gang, Woo Yong Lee, Seong Hyeon Yun, Yong Beom Cho, Jung Wook Huh, Yoon Ah Park, Hee Cheol Kim p. 60-68

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Foley balloons' tamponade is an effective method for controlling massive pelvic bleeding during colorectal surgery Tzu-Chi Hsu p. 69-71

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Molecular characterization of dysplasia : initiated colorectal cancer with assessing matched tumor and dysplasia samples Sungwon Jung, Jong Lyul Lee, Tae Won Kim, Jongmin Lee, Yong Sik Yoon, Kil Yeon Lee, Ki-hwan Song, Chang Sik Yu, Yong Beom Cho p. 72-81

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Outcomes of surgical management of buschke-lowenstein tumor in a Philippine tertiary hospital Sofia Isabel T. Manlubatan, Mark Augustine S. Onglao, Mayou Martin T. Tampo, Marc Paul J. Lopez p. 82-87

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Single-port robot-assisted abdominoperineal resection : a case review of the first four experiences Moon Suk Choi, Seong Hyeon Yun, Jung Kyong Shin, Yoon Ah Park, Jung Wook Huh, Yong Beom Cho, Hee Cheol Kim, Woo Yong Lee p. 88-92

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(Letter to the Editor) Comments on: factors predicting the presence of concomitant enterocele and rectocele in female patients with external rectal prolapse Ingrid Melo-Amaral, Adrian Teran-Cardoza, Cristopher Varela p. 93-94

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참고문헌 (47건) : 자료제공( 네이버학술정보 )

참고문헌 목록에 대한 테이블로 번호, 참고문헌, 국회도서관 소장유무로 구성되어 있습니다.
번호 참고문헌 국회도서관 소장유무
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24 Fernandez J, Gharahdaghi F, Mische SM. Routine identification of proteins from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels or polyvinyl difluoride membranes using matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Electrophoresis 1998;19: 1036-45. 미소장
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32 Brackley KI, Grantham J. Activities of the chaperonin containing TCP-1 (CCT): implications for cell cycle progression and cytoskeletal organisation. Cell Stress Chaperones 2009;14:23-31. 미소장
33 Lim Y, Hong E, Kwon D, Lee E. Proteomic identification and comparative analysis of asymmetrically arginine-methylated proteins in immortalized, young and senescent cells. Electrophoresis 2010;31:3823-33. 미소장
34 Matsuzawa SI, Reed JC. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol Cell 2001;7:915-26. 미소장
35 Taipale M, Tucker G, Peng J, Krykbaeva I, Lin ZY, Larsen B, et al. A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways. Cell 2014;158:434-48. 미소장
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