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Identification and differential expression of microRNAs in Madin-Darby canine kidney cells with high and low tumorigenicities / Jiamin Wang ; Lixia Liu ; Di Yang ; Li Zhang ; Ayimuguli Abudureyimu ; Zilin Qiao ; Zhongren Ma 1

Abstract 1

Abbreviations 1

Background 1

Methods 2

Results 3

Discussion 6

Conclusions 8

Supplementary Information 8

Author contributions 8

References 9

초록보기

Background

Madin–Darby canine kidney (MDCK) cells are widely used for vaccine production, however, the safety of MDCK cells needs to be considered seriously because of high tumorigenicity. Micro RNAs (miRNAs) that are involved in the tumorigenicity of MDCK cells have been never been reported.

Objective

To reveal the role of miRNA in the tumorigenic phenotype of MDCK cell line.

Methods

The miRNA expression profiles of two monoclonal MDCK cells (M09CL and M35CL) with low tumorigenicity and one MDCK cell line (M73P) with high tumorigenicity were characterized and investigated by using small RNA-seq technology.

Results

A total of 5 known miRNAs and 5 novel miRNAs were highly expressed in M73P. In addition, 4 known miRNAs and 4 novel miRNAs were highly expressed in M09CL and M35CL. The target genes of the differentially expressed miRNAs were significantly enriched in several biological processes, and the majority of these genes were involved in pathways in cancer and the MAPK signaling pathway. Through interaction analysis, 4 up-regulated miRNAs (cfa-miR-452, cfa-miR-8826, cfa-miR-224, and cfa-miR-2387) and their crucial target genes related to the tumor regulation network were identified. Results indicated these 4 miRNAs might play crucial roles in the tumorigenesis of MDCK cells.

Conclusion

Our findings, which were based on the functional prediction of miRNAs and target genes, suggested that miRNAs might influence the tumorigenicity of MDCK cells by regulating target genes. Moreover, the results provided important data for understanding the miRNA-mediated regulatory networks that control the tumorigenicities of MDCK cells.

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참고문헌 (51건) : 자료제공( 네이버학술정보 )

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번호 참고문헌 국회도서관 소장유무
1 Abdoli A, Soleimanjahi H, Jamali A, Mehrbod P, Gholami S, Kianmehr Z, Feizi N, Saleh M, Bahrami F, Mokhtari-Azad T (2016) Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production. Biotechnol Lett 38:941–948 미소장
2 Chang M, Lai M, Hong S, Pan B, Huang H, Yang S, Wu C, Sun H, Chuang J, Wang C et al (2018) FGF9/FGFR2 increase cell proliferation by activating ERK1/2, Rb/E2F1, and cell cycle pathways in mouse Leydig tumor cells. Cancer Sci 109:3503–3518 미소장
3 Chen Z, Zhuang W, Wang Z, Xiao W, Don W, Li X, Chen X (2019) MicroRNA-450b-3p inhibits cell growth by targeting phosphoglycerate kinase 1 in hepatocellular carcinoma. J Cell Biochem 120:18805–18815 미소장
4 Dai T, Lin X, Zhao X, Chen X, Wang C, Li X, Shen H, Wang X (2012) MicroRNA-224 targets RKIP to control cell invasion and expression of metastasis genes in human breast cancer cells. Biochem Biophys Res Commun 425:127–133 미소장
5 Ding C, Chen C, Zhang Z, Xiao H, Xie F, Lei L, Chen Y, Mao B, Jiang M, Li J et al (2014) miR-224 promotion of cell migration and invasion by targeting Homeobox D 10 gene in human hepatocellular carcinoma. J Gastroenterol Hepatol 29:835–842 미소장
6 Donis RO, Chen LM, Davis CT, Foust A, Hossain MJ, Johnson A, Klimov A, Loughlin R, Xu X, Tsai T (2014) Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing. Vaccine 32:6583–6590 미소장
7 Enright AJ, John B, Gaul U, Tuschl T, Sander C, Marks DS (2003) MicroRNA targets in Drosophila. Genome Biol 5:1–23 미소장
8 Fiore AE, Uyeki TM, Broder K, Finelli L, Euler GL, Singleton JA, Iskander JK, Wortley PM, Shay DK, Bresee JS (2010) Prevention and control of influenza with vaccines: recommendations of the advisory committee on immunization practices (ACIP), 2010. MMWR Recomm Rep 59:1–61 미소장
9 Fujii H, Takahashi H, Ohyama T, Hattori K, Suzuki S (2002) Evaluation of the school health surveillance system for influenza, Tokyo, 1999–2000. Jpn J Infect Dis 55:97–99 미소장
10 Garten RJ, Davis CT, Russell CA, Shu B, Lindstrom S, Balish A, Sessions WM, Xu X, Skepner E, Deyde V (2009) Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science 325:197–201 미소장
11 Gaush CR, Hard WL, Smith TF (1966) Characterization of an established line of canine kidney cells (MDCK). Proc Soc Exp Biol Med 122:931–935 미소장
12 Gebert L, MacRae I (2019) Regulation of microRNA function in animals. Nar Rev Mol Cell Bi 20:21–37 미소장
13 Genzel Y, Dietzsch C, Rapp E, Schwarzer J, Reichl U (2010) MDCK and Vero cells for influenza virus vaccine production: a oneto-one comparison up to lab-scale bioreactor cultivation. Appl Microbiol Biotechnol 88:461–475 미소장
14 Green IJ (1962) Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells. Science 138:42–43 미소장
15 Gregersen JP, Schmitt HJ, Trusheim H, Bröker M (2011) Safety of MDCK cell culture-based influenza vaccines. Fut Microbiol 6:143–152 미소장
16 Grohskopf L, Olsen S, Sokolow L, Bresee J, Cox N, Broder K, Karron R, Walter E (2014) Prevention and control of seasonal influenza with vaccines: recommendations of the advisory committee on immunization practices (ACIP)-United States, 2014–15 influenza season. MMWR Morb Mortal Wkly Rep 63:691–697 미소장
17 He Z, Xia Y, Pan C, Ma T, Liu B, Wang J, Chen L, Chen Y (2015) Up-regulation of MiR-452 inhibits metastasis of non-small cell lung cancer by regulating BMI1. Cell Physiol Biochem 37:387–398 미소장
18 Hu K, Liang M (2017) Upregulated microRNA-224 promotes ovarian cancer cell proliferation by targeting KLLN. In Vitro Cell Dev Biol Anim 53:149–156 미소장
19 Hu M, Wang F, Song M, Huang Q, Ge B (2017) miR-224 controls human colorectal cancer cell line HCT116 proliferation by targeting Smad4. Int J Med Sci 14:937–942 미소장
20 Ip C, Ng P, Jeong K, Shao S, Ju Z, Leonard P, Hua X, Vellano C, Woessner R, Sahni N et al. (2018) Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies. Nat Commun 9 미소장
21 Jun L, Gad G, Miska EA, Ezequiel AS, Justin L, David P, Alejandro S, Ebert BL, Mak RH, Ferrando AA (2005) MicroRNA expression profiles classify human cancers. Nature 435:834–838 미소장
22 Katz JM, Wang M, Webster RG (1990) Direct sequencing of the HA gene of influenza (H3N2) virus in original clinical samples reveals sequence identity with mammalian cell-grown virus. J Virol 64:1808–1811 미소장
23 Kim EH, Kwon HI, Park SJ, Kim YI, Si YJ, Lee IW, Kim SM, Kim SI, Ahn DH, Choi YK (2018) Generation of a high-growth influenza vaccine strain in MDCK cells for vaccine preparedness. J Microbiol Biotechnol 28:997–1006 미소장
24 Krazinski B, Kowalczyk A, Sliwinska-Jewsiewicka A, Grzegrzolka J, Godlewski J, Kwiatkowski P, Dziegiel P, Kmiec Z, Kiewisz J (2018) IKBKB expression in clear cell renal cell carcinoma is associated with tumor grade and patient outcomes. Oncol Rep 41:1189–1197 미소장
25 Li TT, Wang ZG, Wang SY, He MR, Ye YP, Qi L, Cui YM, Wu P, Jiao HL, Zhang C et al (2013) microRNA-224 promotes cell proliferation and tumor growth in human colorectal cancer by repressing PHLPP1 and PHLPP2. Clin Cancer Res 19:4662–4672 미소장
26 Li Y, Wang F, Lv W, Xie X, Cheng X (2016) Over-expressed miR-224 promotes the progression of cervical cancer via targeting RASSF8. PLoS ONE 11:e0162378 미소장
27 Li W, Li G, Fan Z, Liu T (2017) Tumor-suppressive microRNA-452 inhibits migration and invasion of breast cancer cells by directly targeting RAB11A. Oncol Lett 14:2559–2565 미소장
28 Li T, Jian X, He H, Lai Q, Li X, Deng D, Liu T, Zhu J, Jiao H, Ye Y et al. (2018) MiR-452 promotes an aggressive colorectal cancer phenotype by regulating a Wnt/β-catenin positive feedback loop. J Exp Clin Cancer Res 37 미소장
29 Liu J, Shi X, Schwartz R, Kemble G (2009) Use of MDCK cells for production of live attenuated influenza vaccine. Vaccine 27:6460–6463 미소장
30 Liu J, Mani S, Schwartz R, Richman L, Tabor DE (2010) Cloning and assessment of tumorigenicity and oncogenicity of a Madin-Darby canine kidney (MDCK) cell line for influenza vaccine production. Vaccine 28:1285–1293 미소장
31 Lon MQ, Daley MF, Shannon S, Crane LA, Beaty BL, Jennifer B, Christine B, Miriam L, Allison K (2009) Impact of the 2004–2005 influenza vaccine shortage on pediatric practice: a national survey. Pediatrics 123:186 미소장
32 Love MI, Huber W, Anders S (2014) Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol 15:550 미소장
33 Ma GL, Qiao ZL, He D, Wang J, Kong YY, Xin XY, Wen FQ, Bao SJ, Ma ZR, Wang FS et al (2020) Establishment of a low-tumorigenic MDCK cell line and study of differential molecular networks. Biologicals 68:112–121 미소장
34 Manini I, Trombetta CM, Lazzeri G, Pozzi T, Rossi S, Montomoli E (2017) Egg-independent influenza vaccines and vaccine candidates. Vaccines 5 미소장
35 Miao X, Gao H, Liu S, Chen M, Xu W, Ling X, Deng X, Rao C (2018) Down-regulation of microRNA-224 -inhibites growth and epithelial-to-mesenchymal transition phenotype -via modulating SUFU expression in bladder cancer cells. Int J Biol Macromol 106:234–240 미소장
36 Onions D, Egan W, Jarrett R, Novicki D, Gregersen J (2010) Validation of the safety of MDCK cells as a substrate for the production of a cell-derived influenza vaccine. Biologicals 38:544–551 미소장
37 Papaccio F, Della Corte C, Viscardi G, Di Liello R, Esposito G, Sparano F, Ciardiello F, Morgillo F (2018) HGF/MET and the immune system: relevance for cancer immunotherapy. Int J Mol Sci 19:3595 미소장
38 Percheson PB, Trépanier P, Dugré R, Mabrouk T (1999) A Phase I, randomized controlled clinical trial to study the reactogenicity and immunogenicity of a new split influenza vaccine derived from a non-tumorigenic cell line. Dev Biol Stand 98:127–132 미소장
39 Qiao Z, Yang D, Liu L, Liu Z, Wang J, He D, Wu H, Wang J, Ma Z (2019) Genome-wide identification and characterization of long non-coding RNAs in MDCK cell lines with high and low tumorigenicities. Genomics 미소장
40 Reimand J, Arak T, Adler P, Kolberg L, Reisberg S, Peterson H, Vilo J (2016) g:Profiler-a web server for functional interpretation of gene lists (2016 update). Nucleic Acids Res 44:W83-89 미소장
41 Schild GC, Oxford JS, de Jong JC, Webster RG (1983) Evidence for host-cell selection of influenza virus antigenic variants. Nature 303:706–709 미소장
42 Scott HS, Goodall GJ (2016) A network-biology perspective of microRNA function and dysfunction in cancer. Nat Rev Genet 17:719–732 미소장
43 Shukla P, Vogl C, Wallner B, Rigler D, Müller M, Macho-Maschler S (2015) High-throughput mRNA and miRNA profiling of epithelial-mesenchymal transition in MDCK cells. BMC Genomics 16 미소장
44 Sook-San W, Richard JW (2013) Traditional and new influenza vaccines. Clin Microbiol Rev 26:476–492 미소장
45 Stiles CD, Desmond W, Chuman LM, Sato G, Saier MH (1976) Relationship of cell growth behavior in vitro to tumorigenicity in athymic nude mice. Cancer Res 36:3300–3305 미소장
46 Teferedegne B, Macauley J, Foseh G, Dragunsky E, Chumakov K, Murata H, Peden K Jr (2014) MicroRNAs as potential biomarkers for VERO cell tumorigenicity. Vaccine 32:4799–4805 미소장
47 Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR (2008) Targeted deletion reveals essential and overlapping functions of the miR-17∼92 family of miRNA clusters. Cell 132:875–886 미소장
48 Wang L, Liu W, Zhang Y, Huang X (2017) The miR-224 promotes non-small cell lung cancer cell proliferation by directly targeting RASSF8. Eur Rev Med Pharmacol Sci 21:3223–3231 미소장
49 Wang X, Wang X, Zhu Z, Li W, Yu G, Jia Z, Wang X (2019) Prostate carcinoma cell-derived exosomal MicroRNA-26a modulates the metastasis and tumor growth of prostate carcinoma. Biomed Pharmacother 117:109109 미소장
50 Xu P, Zhang G, Hou S, Sha L (2018) MAPK8 mediates resistance to temozolomide and apoptosis of glioblastoma cells through MAPK signaling pathway. Biomed Pharmacother 106:1419–1427 미소장
51 Yang QL, Kong JJ, Wang DW, Zhao SG, Gun SB (2013) Swine leukocyte antigen-DQA gene variation and its association with piglet diarrhea in large white, landrace and duroc. Asian Australas J Anim Sci 26:1065–1071 미소장

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